Biomedical subjects
Publications and source records attributed to Bronwyn J Battersby.
Novel, porous, functionalised silica particles have been developed with controlled morphology, which promote covalent attachment of fluorescent dyes which can act as an optical barcode.
The creation of enormous libraries of chemicals and their subsequent screening for bioactivity has been accelerated through recent developments in encoding solid supports. The ability to accurately identify the structure of a biomolecule that has exhibited activity is invaluable and is closer to realisation in the advent of smart nanoscience. In this review the evolution of encoding solid supports as platforms for combinatorial synthesis is traced. Current approaches to encoding solid supports are reviewed and their potential for use as supports for the high-throughput screening of split and mix libraries explored. Finally, a brief consideration of the status of the application of encoded libraries is provided including creative chemical and colloidal encoding.
The enormous amount of information generated through sequencing of the human genome has increased demands for more economical and flexible alternatives in genomics, proteomics and drug discovery. Many companies and institutions have recognised the potential of increasing the size and complexity of chemical libraries by producing large chemical libraries on colloidal support beads. Since colloid-based compounds in a suspension are randomly located, an encoding system such as optical barcoding is required to permit rapid elucidation of the compound structures. We describe in this article innovative methods for optical barcoding of colloids for use as support beads in both combinatorial and non-combinatorial libraries. We focus in particular on the difficult problem of barcoding extremely large libraries, which if solved, will transform the manner in which genomics, proteomics and drug discovery research is currently performed.
High-throughput screening (HTS) using high-density microplates is the primary method for the discovery of novel lead candidate molecules. However, new strategies that eschew 2D microplate technology, including technologies that enable mass screening of targets against large combinatorial libraries, have the potential to greatly increase throughput and decrease unit cost. This review presents an overview of state-of-the-art microplate-based HTS technology and includes a discussion of emerging miniaturized systems for HTS. We focus on new methods of encoding combinatorial libraries that promise throughputs of as many as 100,000 compounds per second.