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Biomedical subjects

C A Bennett

Publications and source records attributed to C A Bennett.

30 records · Page 2Linked to original sources
PubMed1992

Clonal characteristics of acute lymphoblastic cells derived from BCR/ABL p190 transgenic mice.

The clonal and immunophenotypic characteristics of blood leukemic cells from BCR/ABL p190 transgenic mice were investigated. All cell populations evaluated in vivo and in vitro had B-lymphocyte progenitor immunophenotypes. Immunoglobulin (JH) rearrangement patterns provided evidence for clonal diversification at different sites in vivo. Multiple clones were established in vitro from two of these mice (nos. 730 and 753). These cells expressed BCR/ABL p190 protein tyrosine kinase (PTK) and were highly malignant on transfer to secondary recipients. Cells independently cloned in vitro shared identical immunophenotypes and clonal IgH rearrangements, but these were distinct from those of the dominant clones in the mouse from which they were derived. Nevertheless, in vitro clones from mouse no. 753 had an abnormal karyotype (chromosome 14 trisomy) in common with the dominant clone in blood, providing evidence for a hierarchy or clonal selection in vivo and in vitro. Two sets of in vitro clones proliferated independently of exogenous growth factors and stroma and released autocrine interleukin 7 growth factor activity. These data provide evidence for rapid divergent clonal evolution and selection of B-cell progenitors initiated by BCR/ABL p190, followed by other, secondary genetic events mirroring similar changes in the equivalent, highly malignant human leukemia Philadelphia (Ph)-positive/B-precursor acute lymphoblastic leukemia (ALL).

Animals↗
PubMed1991

Increased production of growth factors and epidermal growth factor receptor by gastric carcinoma.

Four growth factors and one growth factor receptor have been studied in carcinomas from 22 gastrectomy specimens and compared to non-malignant tissue from the same specimen. cDNA probes for transforming growth factors alpha and beta, platelet-derived growth factor A and B, insulin-like growth factor II and epidermal growth factor receptor were used to assay messenger RNA transcripts for the growth factors by dot hybridization. Increased levels of all the transcripts were found in carcinomas compared to benign tissue (P less than 0.05). No correlation was found between any of the growth factors studied and tumour stage or patient survival. Increased growth factor production by gastric cancers may be important in the pathogenesis of these tumours and further work is required to establish their role.

Adenocarcinoma↗
PubMed1985

Comparison of high and low sodium bicarbonate and acetate dialysis in stable chronic hemodialysis patients.

Eight stable center dialysis patients completed four, 10-week study periods in which either acetate or bicarbonate dialysis was used, each with high or low sodium concentration. During high sodium dialysis, blood pressure was better controlled, weight loss more easily tolerated and dialysis was most satisfactory from the patient's point-of-view with regard to dialysis-associated symptoms. Low sodium dialysis, whether with acetate or bicarbonate, was less satisfactory. In contrast to the beneficial effect of an increased sodium concentration, the use of bicarbonate instead of acetate appeared to make little difference either to the patient's symptoms, to the control of blood pressure or to changes in blood gases or biochemistry. Careful choice of dialysate sodium concentration appears to be important in lessening dialysis side-effects. Substitution of bicarbonate for acetate in chronic stable dialysis patients has comparatively little benefit and the choice can legitimately be made on the basis of cost and technical considerations.

Acetates↗
PubMed1981

Increased levels of threonyl-tRNA synthetase in a borrelidin-resistant Chinese hamster ovary cell line.

The growth of Chinese hamster ovary cells in medium containing reduced concentrations of threonine is inhibited by borrelidin, a macrolide antibiotic. Borrelidin-resistant clones have been isolated after ethyl methanesulfonate mutagenesis. One clone, 1C-1, has a 3-fold increased level of threonyl-tRNA synthetase [L-threonine:tRNAThr ligase (AMP-forming), EC 6.1.1.3] as determined by both activity measurements and antiserum titrations. The levels of four other aminoacyl-tRNA synthetases and of tRNAThr are the same in strain 1C-1 and in the wild-type parent. The phenotype of increased threonyl-tRNA synthetase activity is recessive to wild type in cell hybrids.

Amino Acyl-tRNA Synthetases↗
PubMed1978

Conversion of rat pre-proalbumin to proalbumin in vitro by ascites membranes. Demonstration by NH2-TERMINAL SEQUENCE ANALYSIS.

Rat liver poly(A)-containing RNA was translated in an ascites cell-free system. Labeled protein precipitable by antibody directed against rat serum albumin was identified as pre-proalbumin based on its size and partial NH2-terminal sequence. However, when an ascites membrane fraction was added to the translation reaction, the albumin antibody-precipitable material was smaller than pre-proalbumin. Partial NH2-terminal sequence analysis of this protein revealed that it was proalbumin. Conversion of pre-proalbumin to proalbumin by the ascites membrane fraction was complete and precise--i.e. no serum albumin was observed. Reconstitution in vitro of the processing of pre-proalbumin to its stable intracellular form, proalbumin, provides a method for studying the initial proteloytic event involved in secretion of rat serum albumin.

Amino Acid Sequence↗