Biomedical subjects

C A Cole

Publications and source records attributed to C A Cole.

8 recordsLinked to original sources

PubMed1990

Application of a thermally-reversible polymer-antibody conjugate in a novel membrane-based immunoassay.

We have developed a novel method to immobilize antibodies onto a cellulose acetate membrane using a conjugate of an N-isopropylacrylamide polymer covalently bound to the antibody. When compared with the unconjugated antibody, over 30-fold increase in retention of the antibody on the membrane was observed when it was conjugated to poly (N-isopropylacrylamide). Studies of the polymer-membrane interaction suggest a combination of hydrophobic and ionic forces, especially the former, is responsible for the high retention. We applied this novel immobilization technology in the development of a membrane-based immunoassay.

Antibodies↗

PubMed1989

Sunscreen testing using the mouse ear model.

During the developmental stages of sunscreen formulation it is desirable to have a simple, accurate and inexpensive biological model to test product effectiveness. Another desirable attribute is a quantitative, unbiased response endpoint for evaluation. We have developed such a test system based upon the ear swelling response of hairless albino mice. With this system, irradiation times are greatly reduced; furthermore, the response parameter is metric and can be determined noninvasively with an inexpensive micrometer. Protection factors determined with the mouse ear model show high correlation with the sun protection factors as determined on human subjects (r = 0.92) and were linearly related over a wide range of values. This new method affords a simple, accurate and inexpensive system for evaluation of efficacy and safety of new products.

Animals↗

PubMed1986

Duplex carotid ultrasound.

Technical advances in the field of ultrasound have resulted in the development of a high resolution real-time B-mode imaging system that permits direct Doppler analysis of hemodynamic abnormalities. This system combines both anatomic information and physiological information which permits detailed analysis of the entire spectrum of disease occurring at the carotid bifurcation. Future developments will permit improved management of patients at risk for stroke from carotid vascular disease.

Arteriosclerosis↗

PubMed1986