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Biomedical subjects

C A Fossati

Publications and source records attributed to C A Fossati.

47 records · Page 3Linked to original sources
PubMed1992

Differentiation between active and inactive human brucellosis by measuring antiprotein humoral immune responses.

A preparation of Brucella abortus cytoplasmic proteins was depleted of lipopolysaccharide (LPS) by immunoadsorption with a monoclonal antibody (MAb), BC68, specific for the O antigen of B. abortus smooth LPS. Two enzyme-linked immunosorbent assay (ELISA) systems were developed and used in this study. The first system includes an LPS-free cytoplasmic protein preparation; the second one was based on antigen capture on MAb BC68. By using these systems, we have demonstrated that 94% (33 of 35) of the brucellosis patients studied showed immunoglobulin G antiprotein response and also that all of the patients showed a strong anti-LPS reactivity. Thirty-six serologically positive individuals with no active infection at the time of examination (SPI) were also included. No immunoglobulin G antibodies against proteins were detected in 34 of them (92%), whereas 31 SPI (86%) showed various degrees of anti-LPS reactivity. The use of the LPS-free protein extract in ELISAs made it possible to establish differential reactivity patterns between active and inactive brucellosis.

Antibodies, Bacterial↗
PubMed1991

Human brucellosis: immunoblotting analysis of three Brucella abortus antigenic fractions allows the detection of components of diagnostic importance.

Results indicating that analysis of the immune humoral response of brucellosis patients by immunoblotting provides useful information for the characterization of antigenic fractions of possible diagnostic importance in human brucellosis are presented. Sera of 90 patients were obtained: 23 suffering from chronic brucellosis, 20 from acute brucellosis and 47 belonging to the group of serologically positive individuals (SPI) without clinical evidence of active infection at the time of examination, and 35 healthy volunteers. They were tested against three antigenic fractions: cytoplasmic (CYT), outer membrane (OM) and inner membrane (IM). These fractions, which include virtually all the bacterial protein components, were prepared from Brucella abortus 1119/3 strain by detergent solubilization, enzymatic digestion and ultracentrifugation. Results obtained with these fractions showed the existence of antigens that permit the detection of brucellosis patients and their differentiation from SPI patients, with very high sensitivity.

Antigens, Bacterial↗
PubMed1990

Isolation of a Trypanosoma cruzi antigen by affinity chromatography with a monoclonal antibody. Preliminary evaluation of its possible applications in serological tests.

By affinity chromatography with a monoclonal antibody (163B6), obtained in our laboratory, we have isolated a T. cruzi antigen which could be useful for differential diagnosis of Chagas' disease from leishmaniasis. This antigen, a 52-kD protein, reacted with all sera from Chagas' disease patients tested but not with sera from patients with leishmania, in ELISA. The 52-kD antigen is widely distributed in the Trypanosoma genus since the 163B6 monoclonal antibody reacts with T. rangeli and 8 strains and a clone of T. cruzi epimastigotes.

Animals↗
PubMed1982

Antibodies to poliovirus detected by immunoradiometric assay with a monoclonal antibody.

An immunoradiometric assay (IRMA) for the assay of antibodies to poliovirus antigens is described. Dilutions of the test sera or whole (finger prick) blood samples were incubated with the Poliovirus antigen bound to a solid phase and the specific antibody was detected by the addition of a mouse anti-human IgG monoclonal antibody (McAb), which was itself revealed by iodinated sheep IgG anti-mouse F(ab). We have shown that this technique is suitable for the estimation of IgG anti-poliovirus antibodies induced in children following polio vaccine. The present study shows that SPRIA provides a simple and inexpensive method for serological studies with poliovirus particularly for use in large-scale surveys.

Antibodies, Monoclonal↗
PubMedDate not supplied

Serological investigation of latex allergy in Argentina.

Atopy to latex has been reported in different high-risk groups of subjects in whom it is mainly caused by proteins from natural latex that are responsible for eliciting a variety of clinical symptoms, some of them systemic. Thus, identification of subjects sensitized to latex proteins is of great health importance, because it would become possible to advise them to avoid contact with latex-containing products. Protein extracts from ammoniated latex were prepared by incubation with PBS buffer either with or without detergents, followed by ultracentrifugation. Three immunoenzymic methods were developed (EAST, ELISA, and immunoblotting) to detect the presence of specific IgE antibodies against latex proteins in sera of patients from different groups at risk. The protein content of the latex extracts ranged from 5.3 to 8.8 mg/mL. The prevalence of specific IgE against latex proteins was 9/28 (32.1%) in children with multiple surgeries, 17/98 (17.3%) in health care workers, and 23/123 (18.6%) in outpatients assisted at the Allergy Department. None of the sera belonging to the healthy control group showed the presence of specific IgE. Therefore, this protein extract from latex could be used to detect specific IgE antibodies in serum by immunoenzymic methods. Serologic results for latex-specific IgE found are in accordance with those reported in the literature of other countries.

Adolescent↗
PubMedDate not supplied

Detection of allergens in Aedes albifasciatus mosquito (Diptera: Culicidae) extracts by immunological methods.

Aedes albifasciatus (Diptera: Culicidae) is the most common mosquito species in Argentina and it has been demonstrated to be the vector for some pathogens. The objective of this study was to describe the allergen composition of this mosquito species endemic to Argentina using SDS-PAGE and immunoblotting methods. Sera from mosquito-bite allergic subjects were employed. The protein extracts, obtained from thoraxes containing salivary glands, showed a protein pattern with components of apparent molecular weights ranging from 14 to over 94 kDa. Some of the components could bind IgE in the 16, 20, 30, 36 and 50-67 kDa-zones, whereas the 14 kDa fraction detectable by SDS-PAGE did not behave as an allergen with any positive serum. This protein extract was used to develop in vitro assays to detect the presence of serum-specific IgE against proteins from A. albifasciatus (RAST and ELISA). Thirty-five sera from patients showing local reactions after mosquito bites were tested. The 21 positive sera were from subjects with clinical histories of atopic signs. Through immunoblotting, these sera revealed IgE reactivity against several fractions, mainly in the 16, 20, 30, 36 and 50-67 kDa zones. Comparing the serum IgE reactivity pattern against A. albifasciatus and Aedes aegypti, we observed that the main difference was found in the 14 kDa region where a strong reactivity was seen. The immunoblotting inhibition results indicate that there might be species-unique and species-shared antigens between A. albifasciatus and A. aegypti.

Adolescent↗