Glucuronidase activation: enzyme action at an interface.
The potentiating action of chloroform on bacterial beta-glucuronidase has been shown to increase as the interface area between the two liquid phases increases. Prior extraction of the enzyme with chloroform causes a loss rather than an increase in activity. It is tentatively suggested that the correlation between activity and interface area may reflect a phenomenon of enzyme action at a liquid/liquid interface.
Chloroform↗