Cephalothoracopagus (janiceps) conjoined twins: computerized three-dimensional reconstruction.
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Biomedical subjects
Publications and source records attributed to C A Murdoch.
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Fascicles of human sural (peripheral sensory) nerve were used as external fiducial points (FP) for accurate registration of serial light microscopic sections in three-dimensional reconstruction of human umbilical cord. This paper describes a method for embedding the FPs within the paraffin wax block simultaneously with the specimen to be sectioned. Using a new design of embedding box, the FPs are embedded close to the specimen and are transferred to the slides as part of the tissue sections. Three to four FPs were used to align and scale the serial tissue sections for digitization and computerized reconstruction, using a commercially available software program on IBM-compatible 80386 hardware. A three-dimensional solid surface graphic model of a segment of human umbilical cord was generated.
Cranial neural crest of Xenopus laevis at different stages of development from neurulation to metamorphosis was studied for the expression of the endogenous galactoside-binding lectin of Xenopus using immunocytochemistry. The presence and localization of members of the N-CAM and cadherin cell adhesion families were also investigated. Lectin and the other known cell adhesion molecules are expressed throughout development and their localization patterns change coordinately depending on the development stage. All the molecules colocalize. The results suggest that all of these molecules, including the lectin, may be involved in cranial development, possibly in cellular adhesion.