Biomedical subjects

C A Ray

Publications and source records attributed to C A Ray.

64 records · Page 4Linked to original sources

PubMed1989

Relation of plasma volume change to intensity of weight lifting.

To determine the relation of plasma volume change to intensity of weight lifting, blood samples were obtained from 15 males [mean (+/- SD) age = 22.3 +/- 3.2 yr; height = 176.3 +/- 6.9 cm; weight = 73.0 +/- 11.7 kg] before and over a 60-min period following weight lifting at four different intensities [40, 50, 60, and 70% of one-repetition maximum (1-RM)]. Weight lifting consisted of completing three circuits of four exercises (bench press, bent-over row, arm curl, and parallel squat), with 10 repetitions of each exercise performed over a 30-s period followed by 30 s of rest. Mean (+/- SE) oxygen uptakes (VO2) during weight lifting at the four intensities were 1.31 +/- 0.04, 1.50 +/- 0.07, 1.72 +/- 0.07, and 1.86 +/- 0.08 1.min-1, or 33-47% of treadmill-determined VO2max, respectively. Mean (+/- SE) blood lactates were 6.5 +/- 0.5, 8.7 +/- 0.5, 9.4 +/- 0.6, and 12.0 +/- 0.6 mmol.1(-1), respectively. Mean (+/- SE) heart rates (HR) were 124 +/- 4, 134 +/- 4, 148 +/- 5, and 161 +/- 4 bpm, or 63-82% of treadmill-determined HRmax, respectively. Plasma volume decreased linearly in relation to intensity of weight lifting with mean responses of -7.7 +/- 0.8, -10.7 +/- 0.8, -12.1 +/- 0.9, and -13.9 +/- 0.8% at 40, 50, 60, and 70% of 1-RM, respectively. It was concluded that plasma volume decreases linearly in relation to intensity (%1-RM) of weight lifting and that the relation is similar to that reported for dynamic, low-resistance exercise such as cycling and running.

Adult↗

PubMed1988

Effect of glucose polymer diet supplement on responses to prolonged successive swimming, cycling and running.

Fifteen male endurance athletes were studied to determine the effect of a glucose polymer (GP) diet supplement on physiological and perceptual responses to successive swimming, cycling and running exercise. Thirty min of swimming, cycling and running at 70% VO2max, followed by a run to exhaustion at 90% VO2max was performed after one week of training under two dietary conditions: 1) GP (230 g of GP consumed daily) and 2) placebo (P, saccharin-sweetened supplement consumed daily). During GP, daily carbohydrate (CHO) intake was higher (p less than 0.05) by 173 g or 14% of energy intake than during P, but total energy intake was not significantly different. During 90 min of exercise, CHO utilization and blood glucose were significantly higher under GP than P by an average of 20.2% and 14.5%, respectively, but heart rate, ventilation, oxygen uptake, ratings of perceived exertion, and plasma lactate were not different. Run time to exhaustion at 90% VO2max was significantly longer by 1.2 min (23%) under GP. The results suggest that a GP diet supplement may be of value during endurance exercise by increasing the availability of CHO.

Bicycling↗

PubMed1988

Dramatic limbic and cortical effects mediated by high affinity PCP receptors.

Using Sokoloff's 2-deoxyglucose (2-DG) autoradiographic technique, the psychotomimetic drug, phencyclidine (PCP, "angel dust") dramatically increased metabolism in diencephalic and telencephalic brain regions known to be rich in high affinity PCP receptors. These effects were greatest in the limbic circuit described in 1937 by the neurologist James Papez (mammillary bodies, anterior thalamus, cingulate gyrus, entorhinal cortex, hippocampus, fornix) and the terminal zones of dopaminergic projections. Caudal to the prefrontal cortex, the cerebral cortex developed an anterior-posterior metabolic gradient somewhat similar to that reported in schizophrenia. Brainstem areas were generally unaffected. These data 1) provide functional data to support Papez' assertion that his central limbic circuit may be important in emotional expression, 2) reaffirm the potential importance of dopaminergic function in psychotic-like behaviors, 3) provide an animal model for schizophrenia, and 4) establish that PCP uses high affinity PCP receptor binding sites to express its psychobiological effects.

Animals↗

PubMed1988

A poxvirus-derived vector that directs high levels of expression of cloned genes in mammalian cells.

High levels of expression of cloned genes have been obtained in mammalian cells by using poxvirus-derived insertion/expression vectors. These vectors employ the cis-acting element (CAE I) that directs the transcription of one of the most strongly expressed genes of cowpox virus. This gene (the 160K gene) encodes the 160-kDa protein that is the major component of the A-type cytoplasmic inclusions. Its counterpart in vaccinia virus (VV) is the 94K gene contained in the HindIII A fragment of the viral DNA. Two insertion vectors have been constructed; each is designed to allow cloned genes to be placed immediately downstream of a modified version of CAE I within a poxvirus genome. One vector, p1200, enables the CAE I-cloned-gene constructs to be inserted into the thymidine kinase gene of VV. This vector was used to create a VV recombinant that directed expression of the chloramphenicol acetyltransferase (CAT) gene. The other vector, p2101, enables the CAE I-cloned-gene constructs to be inserted into the VV 94K gene. The prototype of this vector was used to create a VV recombinant that directed expression of a hybrid CAT-lacZ gene. Infection of cultured human cells with these recombinants led to high levels of synthesis of either the CAT gene product or the CAT-lacZ gene product. Each of these proteins was produced in quantities that were easily detected by Coomassie blue staining of total cell proteins resolved by polyacrylamide gel electrophoresis. We estimate that these vectors are capable of directing the synthesis of milligram amounts of gene product per 10(9) mammalian cells.

Animals↗

PubMed1988

High-level synthesis of biologically active reovirus protein sigma 1 in a mammalian expression vector system.

The reovirus cell-attachment protein, which modulates tissue tropism and the nature of the antiviral immune response, is protein sigma 1. This protein is present in reovirus particles in the form of 12 tetramers anchored in the projections or spikes. It is formed in infected cells in very small amounts only. In order to produce larger amounts of it that are necessary for studying its properties in detail, we have cloned it into a mammalian expression vector system that consists of the very strong cowpox virus A-type inclusion body protein gene promoter inserted into the vaccinia virus genome. In cells infected with the resultant recombinant vaccina virus, protein sigma 1 is formed in large amounts (2-3 mg/10(9) cells). We have isolated the native protein and shown that it exists as tetramers that possess cell-binding activity, hemagglutinating activity, and functional epitopes recognized by both polyvalent antisera and monoclonal antibodies.

Blotting, Western↗

PubMed1986

Hemorrhage in lesions caused by cowpox virus is induced by a viral protein that is related to plasma protein inhibitors of serine proteases.

Several recombinant cowpox viruses were constructed and used to identify a viral gene that controls the production of hemorrhage in lesions caused by the Brighton Red strain of cowpox virus (CPV-BR). This gene is located in the KpnD fragment of CPV-BR DNA, between 31 and 32 kilobases from the end of the genome. This position corresponds well with that predicted from analyses of the DNA structures of spontaneously generated deletion mutants. The gene responsible for hemorrhage encodes a 38-kDa protein that is one of the most abundant early gene products. The 11-base-pair sequence GAAAATATATT present 84 base pairs upstream of its coding region is also present upstream of three other early genes of vaccinia virus; therefore, this sequence may be involved in the regulation of transcription. There is extensive similarity between the predicted amino acid sequence of the 38-kDa protein and the amino acid sequences of several plasma proteins that are inhibitors of various serine proteases involved in blood coagulation pathways. This suggests that the viral protein may possess a similar biological activity, which may enable it to effect hemorrhage by inhibiting one or more of the serine proteases involved in the host's normal processes of blood coagulation and wound containment.

Amino Acid Sequence↗

PubMed1979