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Biomedical subjects

C A Rosenbusch

Publications and source records attributed to C A Rosenbusch.

10 recordsLinked to original sources
PubMed1976

[Diagnosis of myocardial infarct by means of a quick determination of the MB fraction of serum creatinephosphokinase].

A rapid method of determining creatine phosphokinase (CPK) isoenzymes using dithiothreitol, a reducing agent which activates the main fractions of the CPK molecule (MM and MB), was assessed in 68 subjects (34 with myocardial infarction, 18 with skeletal muscle lesions, 16 normal). In acute myocardial infarction, the mean peak of CPK-MB in serum increases to 13.3% of the total CPK (range 8.8% to 21%) from the 18th to the 24th hour after onset of symptoms, whereas in skeletal muscle lesions CPK-MB reaches only 1% of total CPK (range 0% to 2.9%), and in normal subjects CPK-MB is absent. This highly significant difference (p less than 0.001) makes it possible to distinguish accurately between myocardial infarction and skeletal muscle lesions (trauma, hematoma, myositis, hypothyroidism). By its sensitivity (100%) and specificity (97%) this rapid and inexpensive method finds its optimal sphere of application in emergency wards and coronary care units.

Creatine Kinase↗
PubMed1975

[Proceedings: Alkaline phosphatases in patients in chronic hemodialysis].

The alkaline phosphatases (AP) have been studied in 28 patients on chronic hemodialysis. Total AP averaged 49 IU/1 while 4 out of the 28 patients exhibited pathologic values (above 60 IU/1). Determination of iso-AP by acrylamide gel electrophoresis revealed the existence of an intestinal band in 50% of cases, thought this is not observable in a control population. The only relationship seen between the presence of the intestinal iso-AP and different parameters concerned the blood groups O and B. Significant increase of total AP is in all cases due to a bone iso-AP. During a hemodialysis session a mean increase of 30% in total AP has been noted. An increase in total AP is observed in only 2 cases out of 17.

Alkaline Phosphatase↗
PubMed1975

[Alkaline phosphatase isoenzymes. Selection of the seperation methods and their diagnostic value].

Polyacrylamide gel electrophoresis (PAGE) of alkaline phosphatase (AP) was performed in 116 patients with elevated AP and in 36 controls. Results were compared with clinical data and with the heat inactivation techniques for AP isoenzyme separation. This latter method is simple but approximate; it is without value in the case of coexistent bone and liver pathology. PAGE gave a clear separation of liver, bile, bone, and intestinal AP isoenzymes. Disagreement with clinical data was rare (6 out of 86) and occurred in cases of associated bone and liver pathology (with 1 exception only), where one of these two abnormalities was not detected. Heat inactivation is useful as a screening test. If the cause of the elevated AP remains unknown after a few simple examinations, PAGE should then be utilized. Although more time-consuming, it is certainly more effective in the separation of AP isoenzymes.

Adult↗