Biomedical subjects

C A de Almeida

Publications and source records attributed to C A de Almeida.

7 recordsLinked to original sources

PubMed2001

Robotic automated clean-up for detection of fumonisins B1 and B2 in corn and corn-based feed by high-performance liquid chromatography.

A high-performance liquid chromatography (HPLC) system with fluorescence detection and an automated on-line solid-phase extraction procedure for fumonisins B1 and B2 in corn and corn-based products is described. Different amounts of strong anion-exchange, C18 and end-capped C18 (C(18 ec)) silicas were tested for sample clean-up. Various HPLC parameters were analyzed. The best methodology was found to be extraction with acetonitrile-water and clean up on C(18 ec) disposable extraction cartridges. The system has the advantage of running in an unattended mode of operation and allows processing of 40 samples without system refuel, performing clean-up, o-phthaldialdehyde derivatization, injection and fumonisin detection by fluorescence detection linked to a computer integrator for automated data processing. Recoveries were performed with corn and corn-based feed samples (n=3) spiked with 0.1, 0.5, 1.0, 5.0 and 10 microg/g. Average recoveries for corn and corn-based feed were, respectively, 92.6 and 88.3% with relative standard deviations (RSDs) of 5.04 and 6.22%, for fumonisin B1 and 91.2 and 89.0% with RSDs of 5.84 and 7.88% for fumonisin B2. Detection limits (S/N=3) for corn and corn-based feed were approximately 0.03 microg/g for fumonisin B1 and 0.05 microg/g for fumonisin B2

Animal Feed↗

PubMed2000

[Dog bite accidents in childhood].

The incidence of dog bites injuries among children aged 0 to 15 years seen at CSE D. Pedro I health center in Ribeirão Preto, Brazil, in the period of 1993 to 1997, was studied. The results show that there is a need to develop and implement educational and preventive programs about the risks and severity of accidents occurred while in contact with dogs.

Adolescent↗

PubMed1999

Comparison of four anthropometric methods of nutritional assessment and evaluation of the agreement between two reference populations.

A total of 841 children, aged 10 days to 5 years, seen at a primary health care centre in Ribeirão Preto, Brazil, were studied in order to compare the methods of Gomez, Waterlow, Shakir, and Kanawati and McLaren and to determine the correlation between the Brazilian and the National Center for Health Statistics (NCHS) reference populations, which are frequently used in Brazil. Anthropometric measurements (weight, height, arm circumference, and head circumference) were made and personal data were obtained in order to evaluate nutritional status according to the above methods. The two reference populations were compared by the method of Gomez. The NCHS and the Brazilian reference populations were concordant. Comparison of the methods of Gomez and Shakir showed a very mild agreement in the evaluation of nutritional status. Comparison of Waterlow 'wasting' with Kanawati & McLaren and with Gomez showed a mild agreement.

Anthropometry↗

PubMed1996

Comparative study of experimentally induced and post-mortem pink teeth.

Forty-eight pink teeth from eight male cadavers, all dying from unnatural causes were studied for the pink tooth phenomenon. Perfusion with whole and lysed blood, at different temperatures was carried out, followed by longitudinal sectioning for histological observation. Another 30 unblemished human teeth were used to reproduce the phenomenon in vitro to help clarify its pathogenesis.

Blood Stains↗

PubMed1994

[Chronic high levels of CK-MB].

We presented two cases of falsely elevated CK-MB due to the presence of a type 2 macro CK, showed by isoenzymes electrophoresis. The association of this CK-MB falsely elevated with some digestive diseases are frequent. Quantification of CK isoenzymes by electrophoresis could be indicated in those cases in which the clinical course or ECG changes do not correlate with the CK-MB values.

Aged↗

PubMed1989

[Left ventricular diverticulum at the subaortic level].

We report a case of a left ventricular diverticulum located immediately under the aortic valve ring. The patient presented both aortic insufficiency and stenosis, and was submitted to valve replacement surgery and closure of the diverticulum. The peculiarity of this case relies on its non atherosclerotic etiology, its unusual location and also because of the calcification of the diverticulum's wall.

Aortic Valve Insufficiency↗

PubMed1989

[Echocardiographic diagnosis of a hydatid cyst localized at the intraventricular septum].

A patient with case of an hydatid cyst located in the interventricular septum is presented. The two-dimensional echocardiography clearly visualized the cystic configuration of the disease, demonstrating its great usefulness for detecting this type of cardiac pathology. The patient underwent surgery with extracorporeal circulation. In this article we also discuss the surgical indications of this pathology.

Adult↗