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C Andreoni

Publications and source records attributed to C Andreoni.

27 records · Page 2Linked to original sources
PubMed1990

Phenotypic analysis of a large number of normal human bone marrow sample by flow cytometry.

Bone marrow aspirates from 48 healthy donors (34 adults, 14 children) were analyzed by flow cytometry (FACS Analyzer) after purification of low-density bone marrow cells (Ld BMC) on a density gradient (d = 1,077) and labelling with 23 anti-hematopoietic cell monoclonal antibodies. Based on physical properties, these Ld BMC could be divided into four different populations called E, My, Mo and L, which comprised 14% +/- 9%, 31% +/- 16%, 10% +/- 5% and 45% +/- 14% of these cells, respectively. The phenotypic analysis of these different populations enabled the identification in E, of erythrocytes (Glycophorin A+, Rhesus D+, but negative for early erythroid differentiation markers such as the transferrin receptor (Tf. R) and the FA6-152 antigen); in My of cells of the myeloid lineage (VIM2+, HLA DR-); in Mo of cells of the monocytic lineage (VIM2+, CD14+) plus some myeloblasts (VIM2+, CD14-, HLADR+) and finally in L of a heterogeneous population including: 1. T lymphocytes labelled to the same extent by CD2, CD3, CD5 and CD6 (28% +/- 10%), B lymphocytes assessed by CD19 and CD20 (12% +/- 8%), Pre-B cells (CD10+ = 8% +/- 7%), less than 5% of "natural killer" cells (CD16+ or Leu7+) and finally, less than 6% of myelomonocytes (CD14+ and/or VIM2+). 2. The erythroid lineage (rhesus D+ = 42% +/- 20%, Tf.R+ and FA6-152+ = 32% +/- 12%). 3. Undifferentiated cells or progenitor cells (CD34+ = 7% +/- 5%). 4. Cells unlabelled by any antibodies (approximately 6%). We observed no difference between bone marrow samples from adults or children, with respect to physical properties, and with all but four immunological markers. A significantly higher proportion of B cells (CD19+ and CD10+) (P less than 0.001) and undifferentiated cells (CD34+ and HLADR+) (P less than 0.02) was observed in children. These data, obtained from a large number of bone marrow samples, could be used to quantify the imbalance of some bone marrow disorders.

Adolescent↗
PubMed1990

Long-term culture of human bone marrow. I. characterization of adherent cells in flow cytometry.

Using long-term culture techniques, we were able to characterize the different adherent cell elements of the in vitro bone marrow microenvironment by flow cytometry. After 2 weeks of culture, four adherent cell subsets, L, MM, F, and M, were distinguished according to their intrinsic cellular characteristics of volume and spontaneous fluorescence. Using four immunological markers, we identified each population as a hematopoietic lymphocytic group (L), CD45+, VIM2-, CD14-; a hematopoietic myelomonocytic group (MM), CD45+, VIM2+, CD14-; a hematopoietic macrophage group (M), brightly autofluorescent, CD45+, VIM2-, CD14+; and a group of fibroblastoid cells (F) with a very high volume, CD45-, VIM2-, CD14-, and collagen III+. Isolation of the different hematopoietic and nonhematopoietic components of long-term bone marrow culture is thus possible using flow cytometry analysis according to the cell characteristic of volume and spontaneous fluorescence alone.

Antigens, CD↗
PubMed1989

Quantitative analysis of cultured thymic reticulo-epithelial cells labelled by different antibodies: a flow cytometric study.

Quantitative measurements of cultured human and murine thymic, and human thymoma reticuloepithelial cells (REC), immunolabeled by different antibodies (Ab) (TE3, TE4, anti-HTLV p19(p19), lu5, K11 and Aks) and by thymic hormones (thymulin and thymosin alpha 1 (Ta1)) within these cells, were performed using a flow cytometric technique. The anti-keratin polyclonal Ab labeled nearly the whole human or murine population. The p19 monoclonal Ab (MoAb), specific for the subcortical/medullary thymic regions, labelled 37-77% of the human REC. The TE3 MoAb, specific for the cortical region, labelled 54-83% of the REC. These percentages suggest that the cultured thymic REC (TREC) had markers of both regions together and therefore that these markers are not absolutely specific to determine their subcortical/medullary or cortical thymic origin. For the three populations there were more cells containing Ta1 than thymulin. The overlap of the percentage of labelled cells suggests that the same cell could synthesize the two hormones and that these hormones could be localized within the TE3 positive cells.

Animals↗
PubMed1989

Spontaneous expression of a low affinity Fc receptor for IgA (Fc alpha R) on human B cell lines.

Expression of receptors for IgA (Fc alpha Rs) was investigated on a panel of 35 human B cell lines by labelling with human secretory IgA (0.5 mg/ml) and flow cytometry analysis after staining with fluoresceinated goat anti-human secretory component and/or anti-alpha chain F(ab')2 fragments. Receptors for IgA could be demonstrated on one out of nine Burkitt's lymphoma cell lines, three out of five myeloma cell lines and five out of 21 lymphoblastoid cell lines. The percentage of Fc alpha R-positive cells within the same B cell line varied upon repeated examination. Human dimeric IgA1 lambda myeloma protein revealed the same number of IgA receptor positive cells as did secretory IgA, whereas monomeric IgA did not bind to Fc alpha R. Detection of Fc alpha R was not inhibited when the tests were carried out in the presence of human dimeric IgG, IgM, asialo-orosomucoid, and secretory component but it was abrogated by pre-treatment of the cells with trypsin. The binding characteristics of Fc alpha Rs were studied on the myeloma cell line Esteve, using 125I-labelled human dimeric IgA and secretory IgA. The binding was dose-dependent with rapid kinetics and specific inhibition by unlabelled secretory IgA. Scatchard plot analysis resulted in an equilibrium constant K ranging from 3.2 to 4.7 x 10(6) M/l. No correlation was observed between Fc alpha R expression and differentiation stage, monoclonality, polyclonality of the cell lines, or Ig class produced by the B cells.

Antibody Affinity↗
PubMed1988

[Analysis of bone marrow by flow cytometry: morphologic and immunologic aspects].

Bone marrow aspirates from 28 healthy donors (18 adults, 10 children) were analysed by flow cytometry (FACS analyser) after purification of low density bone marrow cells (Ld BMC) on a density gradient (d = 1,077) and labeling with 23 anti-hematopoietic cells monoclonal antibodies. Based on physical properties the Ld BMC could be divided into four different populations called E, My, Mo et L including 13 +/- 8%, 33 +/- 15%, 12 +/- 5% and 42 +/- 14% of these cells respectively. The phenotypic analysis of these different populations allowed us to identify in E, erythrocytes (glycophorin A+, Rhesus D+ but negative for early erythroid differentiation markers like the transferrin receptor and/or the FA6-152 antigen); in My, the myeloid lineage (VIM2+, HLADR-); in Mo, the monocytic lineage (CD14+) and some myeloblasts (CD14-, VIM2+, HLADR+) and finally in L, an heterogeneous population including: 1) leucocyte cells, in which 27.3 +/- 9.0% are T cells labelled to the same extent by CD2, CD3, CD5 and CD6, 13.2 +/- 5.9% are B cells assessed by CD19 and CD20, 8.3 +/- 5.7% are Pré-B (CD10+), less than 5% are "natural killer" cells (CD16+ or Leu7+) and finally less than 6% are myelomonocytes (CD14+ or VIM12); 2) the erythroid lineage (Rhesus D+ = 43.7 +/- 12.9%, transferrin receptor and FA6-152+ 36.7 +/- 9.6%); 3) undifferentiated cells or progenitor cells (CD34+ = 6.5 +/- 3.5%); 4) cells unlabelled with any antibodies (approximatively 6%). We have not observed difference between adults and children bone marrow in regard to physical properties properties and all but also immunological markers. Indeed, a significant (p less than 0.02) higher proportion of B cells (CD19 and CD10) was observed in children. These data get from a large number of bone marrow could be used to quantify the imbalance of some bone marrow disorders.

Adolescent↗