Biomedical subjects

C B Stephensen

Publications and source records attributed to C B Stephensen.

40 records · Page 3Linked to original sources

PubMed1987

Comparison of noninvasive breath hydrogen test for gastric acid secretion to standard intubation test in adults.

We have developed a noninvasive test for gastric acid secretion based on the reaction of ingested magnesium metal with gastric acid to produce hydrogen gas, which is excreted in exhaled air and belches. This test was compared to the standard intubation test by performing both, on different days, in 36 adult Peruvian outpatients referred for gastric analysis; the correlation coefficient for this comparison was 0.71. The new test was repeated in nine subjects, and the resulting test-retest correlation coefficient was 0.83. The new test thus compares favorably with the standard intubation test. Because it is also noninvasive and should therefore be more acceptable to subjects, this new test may be useful for clinical evaluation and research studies on subjects in whom intubation would be difficult or unacceptable.

Adult↗

PubMed1987

Comparison of noninvasive breath hydrogen test for gastric acid secretion to standard intubation test in infants and young children.

We have developed a noninvasive test of gastric acid secretion for use in infants and young children. It is based on the reaction of ingested magnesium metal with gastric acid to produce hydrogen gas, which is excreted in exhaled air and belches. This test was compared to the standard intubation test by performing both, on different days, in 20 children (5-37 months of age) without known gastric disease. The results of the two tests compared well: correlation coefficients were 0.78, 0.75, or 0.72 when exhaled air was collected for a total of 56, 81, or 31 min, respectively. Because the test is noninvasive, it should be more acceptable to children and their parents than the intubation test and thus could be used to conduct studies which are currently impractical.

Acute Disease↗

PubMed1985

Liberation of hydrogen from gastric acid following administration of oral magnesium.

We are in the process of developing a noninvasive test for gastric acid secretion based on the reaction of orally administered magnesium metal with gastric acid: Mg + 2HCl in equilibrium with MgCl2 + H2. We hypothesized that the hydrogen gas thus evolved could be detected in exhaled air and belches and that the amount of hydrogen released could be related to the amount of acid in the stomach. To validate this hypothesis, we gave magnesium to two groups of young adult volunteers following either betazole stimulation or cimetidine inhibition of acid secretion. In group I we gave subcutaneous betazole and gave magnesium in doses from 10 to 200 mg. In group II we gave oral betazole and used a constant dose of 150 mg of magnesium. In both groups we consistently detected significant increases in breath and belch hydrogen following magnesium in the betazole-stimulated volunteers. This response was blocked by cimetidine. The magnitude of the response was related to the magnesium dose, with 150 mg appearing to induce a maximum response. Administration of oral magnesium up to 200 mg was not associated with any untoward effects. We conclude that magnesium led to the release of hydrogen gas in vivo and that the quantity of hydrogen gas recovered was related to the amount of gastric acid. With further development, this principle might be used to develop a simple noninvasive test for gastric acid secretion.

Adult↗

PubMedDate not supplied

Interferon alters intracellular transport of vesicular stomatitis virus glycoprotein.

Double-label immunofluorescence staining studies in virus-infected subclone 11 of LB cells indicated that almost all of the vesicular stomatitis virus (VSV) glycoprotein (G) was plasma membrane-associated during the logarithmic phase of virus replication. In contrast, treatment with interferon (IFN) resulted in inhibition of VSV-G transport, so that almost all of the G remained associated with the Golgi complex (GC) at comparable times after infection. In both IFN-treated and control cells, G was resistant to treatment with the enzyme endo-beta-N-acetylglucosamine H (endo H) indicating that the bulk of the G had reached the trans compartment of the GC.

Animals↗