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Biomedical subjects

C Barefoot

Publications and source records attributed to C Barefoot.

8 recordsLinked to original sources
PubMed1976

Tissue distribution of bovine 125I-superoxide dismutase in mice.

Intravenously injected 125I-bovine superoxide dismutase in mice is essentially cleared from organs in 24 h. The 125I activity per g of tissue at 1 h varies among organs with the brain and kidneys showing the lowest and highest levels, respectively. Within tissues, degradation occurs so that, at 1 h after administration, 5-74% of the 125I activity remains associated with the enzyme, depending on the tissue. A small amount of the labelled enzyme is found in washed cells of thymus, lung, spleen, liver and brain.

Animals↗
PubMedDate not supplied

Red blood cell-bound C3d in normal subjects and in random hospital patients.

Red blood cells (RBC) drawn into EDTA from 103 normal adults, 26 newborn infants, and 203 randomly selected hospital patients, and into citrate-phosphate-dextrose (CPD) from 87 Red Cross donors, were evaluated for RBC-bound C3d by agglutination with anti-C3d and by a radiolabeled anti-antiglobulin technique. Positive agglutination reactions were observed only with RBC from 26 hospital patients; however, by the radiolabeled antiglobulin method, C3d was demonstrable on all RBC, but its amount varied widely among different subjects. A normal range for RBC-bound C3d was established. RBC-bound C3d levels were not related to the age (18-88 years) or sex of the subjects and remained unchanged over 9 months. Red blood cells taken into CPD had more RBC-bound C3d than cells taken into EDTA; cord RBC had less than adult RBC. In 39 percent of the hospital patients (excluding those with autoimmune hemolytic anemia) RBC-bound C3d was above the normal range and a good correlation was found between values obtained by the radiolabeled anti-antiglobulin method and by agglutination titration scores. There was a threshold level of RBC-bound C3d below which agglutination reactions were negative. Evaluation of RBC-bound C3d in health and disease is important for determining optimal characteristics of anticomplement antiglobulin reagents.

Adolescent↗
PubMedDate not supplied

Red blood cell-bound C3d in selected hospital patients.

A radioactive anti-antiglobulin technique was used to measure C3d bound to the red blood cells of 227 hospitalized patients in 129 patients, with a wide variety of diseases, normal levels of RBC-bound C3d were found. Seventy-two patients had moderately elevated RBC-bound C3d; they generally did not have autoimmune hemolytic anemia but had diseases in which complement is thought to be activated. Patients with markedly elevated RBC-bound C3d (26 patients) usually had autoimmune hemolytic anemia with a positive antiglobulin test. Some patients with only moderately elevated levels of RBC-bound C3d had autoimmune hemolytic anemia and a negative antiglobulin test in individual patients the level of RBC-bound C3d correlated with both the severity of disease and the response to treatment. RBC-bound C3b was detected in two patients with a very high level of RBC-bound C3d. This study provides background data for assessing the significance of complement activation and fixation to RBC in health and disease.

Anemia, Hemolytic, Autoimmune↗