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C Basile

Publications and source records attributed to C Basile.

At least 55 records · Page 3Linked to original sources
PubMed1984

Total parathyroidectomy and delayed parathyroid autotransplantation using a simplified cryopreservation technique: human and animal studies.

Following earlier studies using a simplified cryopreservation method in rats, we report the successful use of this technique in a hemodialysis patient who had previously undergone total parathyroidectomy. In additional studies, parathyroid glands from rats were conserved by this rapid freezing procedure after total parathyroidectomy. Six weeks after parathyroid autotransplantation the animals were submitted to a low-calcium diet and their parathyroid gland function compared to that of rats that had previously undergone sham operation, immediate autotransplantation, or delayed autotransplantation using the classical cryopreservation method. With both cryopreservation techniques, plasma calcium, phosphorus, and 1,25 (OH)2 vitamin D concentrations after low-calcium diet were similar although results were less satisfactory than with immediate autotransplantation or after sham operation. We suggest that this simplified cryopreservation technique, developed in rats, yields functioning parathyroid gland tissue and can be successfully used in the human.

Adult↗
PubMed1983

One-hour and half, high-surface-area haemodiafiltration: a feasibility study.

In order to explore the limitations of further shortening of dialysis time, six uraemic patients underwent three different dialysis treatments: haemodiafiltration (HDF) of 180 min with a polyacrylonitrile membrane (1 m2); standard haemodialysis (SH) of 270 min with a cuprophan membrane (1 m2); 90 min, 2 m2 haemodiafiltration (H HDF) with two RP6 in series, dialysate coursed through the two dialysers in parallel and postdilution reinjection of 220 mEq/L of Na+ and 100 mEq/L of bicarbonate after each dialyser. Clearances were extremely more elevated in H HDF than in SH; urea extraction index was significantly higher in SH, but inulin extraction index was significantly lesser in SH than in H HDF.

Blood Flow Velocity↗
PubMed1982

Parathyroid function and lipid metabolism in the rat.

The present investigation in experimental animals was designed to obtain further insight into the possible role of parathyroid hormone (PTH) in lipid metabolism. An endogenous hyperparathyroid state was induced in rats by a calcium-poor diet. This type of hyperparathyroidism was associated with a significant increase in serum cholesterol and triglyceride concentrations, a significant decrease in the serum clearance rate of intravenously infused Intralipid and a significant increase in hepatic tissue triglyceride content as compared to the values obtained in control animals. An exogenous type of hyperparathyroidism (parathyroid extract injections) was also accompanied by a slight increase in serum cholesterol and triglyceride concentrations as well as a decrease in plasma postheparin lipolytic activity (PHLA), but the changes did not reach the level of statistical significance. In the aparathyroid state, a significant decrease in serum cholesterol and triglyceride concentrations and a significant increase in plasma PHLA were observed as compared to control rats. In additional studies, it could be demonstrated that the endogenous hyperparathyroid state was not associated with increased intestinal absorption of [3H]triolein as compared to control animals. Furthermore, the hepatic triglyceride secretion rate in these hyperparathyroid rats was significantly lower than in normal rats. In conclusion, hyperparathyroidism induces an increase in serum cholesterol and triglyceride concentrations, the latter being due to decreased peripheral removal. The aparathyroid state induces opposite changes. Thus, normal parathyroid function is required for normal lipid metabolism in the rat.

Animals↗
PubMed1980

Carnitine improves lipid anomalies in haemodialysis patients.

51 chronic haemodialysis patients with hypertriglyceridaemia were given a daily oral dose of 2.4 g D,L-carnitine for 30 days to investigate a possible hypolipaemic effect. After 30 days' D,L-carnitine treatment the mean (+/- SEM) serum triglyceride concentration had decreased significantly from 3.50 +/- 0.39 to 2.87 +/- 0.27 mmol/l. Serum total cholesterol did not change. However, HDL cholesterol increased significantly from 0.89 +/- 0.05 to 1.35 +/- 0.07 mmol/l. This decrease in serum triglycerides and return of HDL cholesterol to normal levels in haemodialysis patients may be the result of correction of carnitine deficiency. Such treatment could reduce the risk factors for atherosclerosis and coronary-artery disease in uraemic patients.

Adult↗
PubMed1980

Improvement of post-heparin lipolytic activity (PHLA) after haemofiltration, in uraemic patients.

UNLABELLED: The decrease of serum PHLA in uraemic patients has been attributed to toxic effects of 'middle molecules' on lipoprotein lipase. Haemofiltration could improve PHLA by increasing 'middle molecule' clearance. Serum triglyceride concentration decreased and PHLA improved in three uraemic patients who received haemofiltration during three weeks as the initial treatment schedule. Activated PHLA of a healthy subject failed to be inhibited by uraemic sera and uraemic plasma ultrafiltrates obtained in vitro using polyacrylonitrile and cuprophane membranes. CONCLUSION: haemofiltration is able to improve PHLA and to restore normal serum triglyceride concentrations. The reported toxicity of uraemic serum may affect metabolic steps preceding the activation of the circulating lipolytic enzymes.

Blood↗
PubMed1980

[Hyperparathyroidism and anemia: experimental study in the rat (author's transl)].

Red blood cell (RBC) production has been explored in hyperparathyroid as compared to normal rats. The hyperparathyroid state was obtained by feeding the rats a calcium-deficient diet during 30 days. A significant decrease (p less than 0.001) in reticulocyte count and in erythrocyte 59Fe incorporation was observed in hyperparathyroid rats when compared to control animals. A comparable inhibition of erythrocyte 59Fe incorporation in hyperparathyroid rats was found when the study was performed after stimulating bone marrow activity by acute arterial hemorrhage. These results of decreased RBC production using a model of endogenous hyperparathyroidism could be confirmed in additional experiments using a model of exogenous hyperparathyroidism. In this model, the rats received either parathyroid extract (3 x 10 USP/day during 8 days) or vehicle only. Again, a decrease in erythrocyte 59Fe incorporation was observed in hyperparathyroid animals when compared to control animals. It is concluded that the hyperparathyroid state is accompanied by a decrease of RBC production in the rat. It is probable that a similar pathogenesis underlies the anemia of patients with primary or secondary hyperparathyroidism.

Anemia↗
PubMed1980

Isoelectrofocusing of organic esterases in the postnatally developing and adult ovoviviparous teleost, Poecilia reticulata.

In the ovoviviparous teleost, Poecilia reticulata, the esterases give rise to around 13 variants when separated by electrofocusing. These variants show histochemical characteristics (e.g. substrate utilization and sensitivity to inhibiting substances) which can be related to postnatal developmental stages and to adult sex. A few variants, moreover, appear to be typical of the stage examined (newborn, young, adult male and female). Very few variants can be classified by the criteria of Masters and Holmes (1975).

Animals↗
PubMed1980

Successful parathyroid gland autotransplantation in the rat using a simplified cryopreservation technique.

In the present work a simplified technique for cryopreservation of parathyroid glands was compared to the generally used technique needing a programmed freezer. Four groups of rats had total parathyroidectomy. In two of them the glands were cryopreserved and reimplanted 10 days after ablation. The other two groups had, respectively, immediate parathyroid autotransplantation and no autotransplantation. During a follow-up of 60 days, plasma calcium remained low and plasma phosphorus elevated in all aparathyroid rats of the last group. In the group of rats with immediate parathyroid reimplantation, both mean plasma calcium and phosphorus values rapidly returned to the normal values observed before operation. In the rats that had the simplified parathyroid cryopreservation technique, mean plasma phosphorus also returned to the normal range and mean plasma calcium to nearly normal. However, in the group of rats with the classical cryopreservation method, the mean plasma calcium and phosphorus levels remained far from normal in the majority of animals. It was concluded that parathyroid glands of the rat could be successfully autografted after previous freezing using a simplified cryopreservation technique. The results obtained were comparable with, even though not as perfect as, that observed after immediate autotransplantation. If suitable for human parathyroid gland fragments, this technique would allow a more general application of parathyroid tissue cryopreservation.

Animals↗