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Biomedical subjects

C Bentivoglio

Publications and source records attributed to C Bentivoglio.

28 records · Page 2Linked to original sources
PubMed1989

Further characterization of the impaired protective function in mice fed with lipid diet.

Female mice were maintained on lipid diet for 20 days. The nonspecific and immunological defense capability was determined by in vitro and in vivo methods. It was found that mice held mostly on a lipid diet demonstrate an all-round lowered response. Following 20 days of lipid diet the splenocytes exhibit: (1) an inversed lipid-protein ratio; (2) an inability to respond to sheep erythrocytes; (3) a reduction in [3H] thymidine incorporation in splenocytes stimulated with lipopolysaccharide (LPS) or with concanavalin A; (4) a reduction in the number of cells bearing surface immunoglobulins in splenocytes stimulated with LPS; (5) an inhibition of phagocytosis and intracellular killing in macrophages; (6) a lowering in granulocyte chemotaxis and adherence capacity; (7) a higher mortality to LPS after loading with galactosamine; and (8) a lowered complement activity even following LPS activation.

Animals↗
PubMed1988

Phagocytosis of bacterial aggregates by granulocytes.

A study was conducted on the granulocytic phagocytosis of bacterial aggregates obtained under ideal environmental conditions. For the strains studied, aggregation was favored by low salt concentrations, low pH and temperatures between 30 degrees C and 40 degrees C. Our results show that the phagocytic capacity of granulocytes depends on the type and size of these aggregates. Those formed by a smaller number of cells are more easily phagocytized than the larger ones.

Animals↗
PubMed1988

The action of LPS porins and peptidoglycan fragments on human spermatozoa.

This study examines the action of the cell wall components of enterobacteria on the vitality of human spermatozoa. Lipopolysaccharides extracted from Escherichia coli K12 killed about 80% of the spermatozoa at a concentration of 50 micrograms/ml. Porins extracted from E. coli, Proteus mirabilis and Salmonella typhimurium killed between 80% and 100% of the spermatozoa at a concentration of 50 micrograms/ml. Muramic acid and N-acetylmuramic acid caused about 60% mortality at a concentration of 50 micrograms/ml. The possibility that the products of cellular lysis in the course of gram-negative infections cause temporary sterility is discussed.

Bacterial Outer Membrane Proteins↗
PubMed1987

Phagocytosis of bacteria after exposure to macrophages or lymphocytes.

In our present study we have examined the phagocytosis by polynucleates towards bacteria exposed to macrophages or lymphocytes. Our results show that for almost all the bacterial strains, the contact with lymphocytes determines changes in hydrophobicity correlated to variations in the possibility of being phagocytized and of more significant intracellular killing with respect to bacteria treated with macrophages.

Animals↗
PubMed1985

Modifications of surface properties in some enteropathogenic serogroups of Escherichia coli.

Studies have been carried out on protein and fatty acid patterns present in the outer layers of E. coli strains which are considered pathogenic in relation to their serogroup. The patterns were related to surface properties of hydrophobicity of the same cells and to their resistance to phagocytosis. The results obtained demonstrate the presence in the pathogenic serogroups of a proteic band of high molecular weight as well as differences in the percentages of fatty acids with respect to the non-pathogenic serogroups. Furthermore, these serogroups are more resistant to phagocytosis; this resistance seems to be correlated to a greater surface hydrophilia of the pathogenic serogroups, leading to different relations of the lipidic and protein fraction of the outer layers.

Bacterial Outer Membrane Proteins↗
PubMedDate not supplied

Immunological response in mice after long-term stimulation with cell wall antigens from Brucella melitensis.

The continuous stimulation of the immune system using cell wall antigens from Brucella melitensis was found to cause both quantitative and qualitative changes in circulating lymphocyte populations in mice. Animals were inoculated in the hind legs with antigens on alternate days for varying lengths of time. During a two-month period, we saw a higher number of circulating lymphocytes, with an increase in the number of CD4+ cells (L3T4+) and B lymphocytes (I-Ad). After two months, a drop in the overall number of circulating lymphocytes occurred, with a decrease in CD4+ cells and an increase in CD8+ cells. During the first two months, we observed a size increase in popliteal lymph nodes and an elevated humoral response. The response then waned with the declining CD4+ cells. In the first two months, the treated animals also showed an in vitro response to two mitogens, concanavalin A and lipopolysaccharide and to the cell wall fraction, after which the treated animals showed a decreased response.

Animals↗
PubMedDate not supplied

The effects of head injury in children on neuropsychological and behavioural functioning.

In a prospective study 76 children were divided into three groups on the basis of severity of head injury as defined by the Glasgow Coma Scale and duration of increased intracranial pressure. The children were administered a neuropsychological test battery and behavioural ratings were made by parents and teachers at three intervals: time of hospital discharge and 3 and 9 months post-initial testing. There were cognitive deficits related to severity of injury with the greatest difference in abilities observed between the severe and the other two groups. The greatest differences in skills were on the Performance IQ and timed tests of visual-motor speed and co-ordination. The greatest improvement in skills occurred in the first 3 months post-injury. Several children in coma for up to 4 weeks were able to obtain normal IQ scores. In the mild and moderate injury groups very few had behavioural change while in the severe group approximately 90% had one learning or adjustment difficulty and 40% had three or more problems.

Achievement↗
PubMedDate not supplied

Th1-Th2 response in hyperprolactinemic mice infected with Salmonella enterica serovar Typhimurium.

Prolactin (PRL) is a pituitary hormone and a cytokine known to regulate several physiological functions. It plays a role in modulating the immune system of rodents and humans. A hormonal protection against listeria and salmonella infections has been previously ascribed to effects of PRL on immunocompetent cells. Here, the role of PRL in the Th1-Th2 response was evaluated based on the pattern of cytokines release by splenocytes from hyperprolactinemic mice infected with Salmonella enterica serovar Typhimurium. Hyperprolactinemia by pituitary graft reduced the number of bacteria in spleens of in vivo infected mice. Modulation of Th1 (IFN-gamma, IL-12) and Th2 (IL-4, IL-10) cytokine production by splenic cells was found. Our results indicate that PRL can up-regulate IFN-c and IL-12 secretion in response to salmonella infection, confirming its in vivo immunostimulatory effect and suggesting hormonal participation in the genesis and sustenance of the Th1 response.

Animals↗