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C Beyer

Publications and source records attributed to C Beyer.

At least 19 recordsLinked to original sources
PubMed1992

Sex differences of hypothalamic prolactin cells develop independently of the presence of sex steroids.

There is evidence for a hypothalamic prolactin (PRL) system that expresses sexually dimorphic traits. The aim of this in vitro study is to gain an insight into the process of sexual differentiation of hypothalamic PRL cells. In particular, we wanted to determine whether sexual differentiation of these cells can occur independently of the surge of gonadal testosterone which, in the male rat embryo, takes place at embryonic day (E) 18 and is commonly believed to start the critical period of sexual differentiation of the brain. Gender-specific cell cultures were prepared from E 14 or E 17 rat diencephalon and raised in the absence of gonadal steroids. After 10 days in vitro, numbers of PRL-immunoreactive (IR) cells and PRL levels were quantified by immunocytochemistry and Western blotting, respectively. Numbers of PRL-IR cells and PRL levels were 2-3 times higher in cultures prepared from female than from male embryos of either age. It is concluded that sexual differentiation of hypothalamic PRL cells starts well before the generally acknowledged onset of the critical period and may proceed independently of the action of gonadal testosterone. Besides gonadal steroids, other mechanisms, such as cell-intrinsic realization of a sex-specific genetic program, may be responsible for initiating the development of sexually dimorphic neuronal phenotypes.

Analysis of Variance↗
PubMed1992

Visceral and postural reflexes evoked by genital stimulation in urethane-anesthetized female rats.

The present study describes several muscular reflexes produced by genital stimulation, the nerves that subserve them, and the visceral and postural effects induced by these reflexes. Electrical stimulation of the iliococcygeus (ic) and pubococcygeus (pc) (striated) muscles produced movement of the vaginal orifice and wall, membranous urethra, tail and pelvis. Electrical stimulation of the psoas major (pm) or iliacus (i) (striated) muscles produced movements of the lumbar vertebrae and extension of the ipsilateral hindlimb. Sensory mechanostimulation elicited responses of these muscles as follows: stimulation of the perineal skin, clitoral sheath or distal vagina produced reflex contraction of the ic and pc muscles. Stimulation of the cervix produced reflex contraction of the pm and i muscles and also blocked the above reflex contraction of the ic and pc muscles. Both the cervical stimulation-induced blockage of the ic and pc reflex response, and the cervical stimulation-induced activation of pm and i muscles was prevented by bilateral transection of the viscerocutaneous branch of the pelvic nerve. Based on the above observations, it is proposed that stimulation of the vaginal surface of the cervix resulting from penile intromission and/or seminal plug deposition during mating behavior in the rat may reflexively active pm and i, thereby contributing to the hindleg postural rigidity and lordotic dorsiflexion that are characteristic of the normal mating posture in female rats.

Abdominal Muscles↗
PubMed1992

Sex steroids do not alter sex differences in tyrosine hydroxylase activity of dopaminergic neurons in vitro.

In order to distinguish the effects of genetic sex from those of sex hormones on the sexual differentiation of dopaminergic neurons, catecholamine synthesis was studied in gender-specific cultures of embryonic day-14 rat diencephalon. In addition to embryos from normal dams, embryos were used whose mothers had been treated with the estrogen antagonist tamoxifen or the testosterone antagonist cyproterone acetate on days 12 and 13 of gestation. Cultures from embryos of untreated dams were fed daily with a medium containing 17 beta-estradiol or testosterone. After 10 days in vitro, cultures were immunostained for tyrosine hydroxylase and the accumulation of dihydroxyphenylalanine (DOPA) was measured in the presence of the DOPA decarboxylase inhibitor NSD 1015. Rates of DOPA synthesis, unlike the numbers of tyrosine hydroxylase-immunoreactive neurons, were markedly higher in female cultures under all experimental conditions. Treatment of dams with antisteroids prior to removal of the embryos had no influence on these results. Treatment of cultures with both steroids decreased DOPA formation in a dose-dependent manner without altering the sex difference. These results suggest that cultured diencephalic dopaminergic neurons develop sex differences in the activity of tyrosine hydroxylase. This sexual dimorphism is initiated independently on the activity of gonadal steroid hormones. Sex hormones exert an additional modulatory influence on the activity of the enzyme but do not abolish or reverse sex differences. Therefore, the concept of a purely epigenetic mode of sexual differentiation of the mammalian brain needs to be broadened to incorporate other mechanisms, such as the cell-autonomous fulfillment of a sex-specific genetic program.

Animals↗
PubMed1992

Copulatory analgesia in male rats ensues from arousal, motor activity, and genital stimulation: blockage by manipulation and restraint.

The effect of copulation on the vocalization threshold to tail shock (VTTS) was assessed in freely-moving, sexually experienced, Wistar male rats. Mean VTTS during the first copulation was 40% above the baseline values and slightly decreased during the first postejaculatory interval (PEI; 28% above baseline). VTTS mean values further increased during the second copulatory series (93% above baseline and 63% during the PEI). Testing at the same intervals in noncopulating rats did not induce analgesia. VTTS values also increased after the display of either one ejaculation (E), five intromissions (I), or five mounts (M), the analgesia persisting for at least 20 min. Analgesia following M was smaller and shorter than that observed after either I or E. Analgesia developed gradually during copulation since VTTS values after five I were significantly higher than those after one I. The incidence of vocalizations to suprathreshold shocks (STS, 20% above the VTTS) occurring during various phases of copulation was also studied to determine the onset and short-term fluctuations of copulatory analgesia. The proportion of STS inducing vocalizations decreased from 91% (mean of individual proportions) before copulation, to 24% during copulation, and to 25% during the PEI. A maximal reduction in the proportion of vocalizations to STS was found during the last third of copulation. Nearly all vocalizations to STS during copulation occurred when subjects were quiescent, while few or no vocalizations occurred when rats were engaged in sexual activity, i.e., during M, I, or penile grooming. In contrast to the above-mentioned data, no significant analgesia was observed using the tail-flick latency test (TFL) following either E, five I, or five M.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMed1992

Chin-marking behavior in male and female New Zealand rabbits: onset, development, and activation by steroids.

Chin marking (chinning) frequency was determined daily in 25 male and 24 female New Zealand rabbits aged 31-150 days. Chinning appeared earlier in females (mean +/- SD = 41 +/- 16 days) than in males (47 +/- 13 days). Between days 30-50, females displayed chinning more frequently than males. Thereafter, chinning increased steadily in both sexes up to day 100. Chinning curves levelled on days 100 and 140 in males and females, respectively. Profile analysis of male and female chinning curves showed significant differences in their slopes and in their population means (p < 0.001). At 7 months of age (days 210-224), both sexes displayed adult chinning frequencies: mean of means +/- SD = 103 +/- 18 and 79 +/- 14 marks/10 min in males (n = 8) and females (n = 8), respectively. The administration of testosterone propionate (TP, 1 mg/day) or estradiol benzoate (EB, 1 microgram/day) to males and females, respectively, from days 31-50, stimulated higher chinning frequencies than those displayed by untreated animals. Results suggest that chinning frequency increases throughout development largely as a consequence of a concomitant increase in sex steroid secretion.

Aging↗
PubMed1992

Administration of AP5, a glutamate antagonist, unmasks glycine analgesic actions in the rat.

The effect of intrathecal (IT) injection of glycine alone or in combination with 2-amino-5-phosphonopentanoate (AP5) on two nociceptive tests--the vocalization threshold to tail-shock (VTTS) and the tail-flick latency (TFL)--was studied in ovariectomized Sprague-Dawley rats. IT injection of 400 micrograms glycine induced a nonsignificant decrease, that is, in comparison with saline, in both nociceptive thresholds. IT AP5 (10 micrograms) provoked a slight but significant increase in both nociceptive thresholds within the first 15 min postinjection. Combination of both glycine (400 micrograms) and AP5 (10 micrograms) produced marked and prolonged analgesia in both tests, which was significantly different from that obtained with AP5 alone. The results suggest that IT glycine acting through the strychnine-sensitive Gly1 receptor produces analgesia provided its effect on the Gly2 receptor linked to the NMDA receptor is prevented by an antagonist.

2-Amino-5-phosphonovalerate↗
PubMed1992

Diencephalic GABAergic neurons in vitro respond to prolactin with a rapid increase in intracellular free calcium.

In order to analyze the feedback action of prolactin (PRL) on the hypothalamus on the cellular level, we used primary cultures of rat embryonic diencephalon to measure the calcium response of individual neurons to PRL by means of fast fluorescence photometry. The cultures were subsequently stained with antibodies against the neuronal marker MAP-2, glutamic acid decarboxylase (GAD) or tyrosine hydroxylase (TH). PRL caused a rapid rise of intracellular free Ca2+ in a specific type of GABAergic neuron characterized by a spindle-shaped bipolar morphology and immunoreactivity to MAP-2 and GAD but not to TH. It is concluded that a subpopulation of hypothalamic GABAergic but not dopaminergic neurons react to PRL with a rapid increase in intracellular free Ca2+. These data are compatible with the assumption of a rapid negative feedback regulation of the secretion of PRL from the pituitary mediated by tuberoinfundibular GABAergic neurons.

Animals↗
PubMed1992

A new carbamazepine metabolite in uraemic filtrate.

1. After administration of carbamazepine (Tegretal) to an epileptic patient suffering also from uraemia, a previously unknown metabolite was detected in the haemofiltrate. It was identified as the 10,11-dihydro-10-hydroxy-carbamazepine-O-beta-glucuronide. 2. Its identification was accomplished mainly by electron impact (EI), liquid secondary ion (LSI) mass spectrometry and n.m.r. spectroscopy.

Carbamazepine↗
PubMed1991

Cells from embryonic rat striatum cocultured with mesencephalic glia express dopaminergic phenotypes.

To study region-specific transmitter phenotype expression, cells of embryonic day 14 (E14) rat neostriatum (ganglionic eminence plus cortical plate) or of the substantia nigra (ventral mesencephalon) were cultured on glial cells either from substantia nigra or neostriatum (E21). By antityrosine hydroxylase immunocytochemistry, immunoblotting of tyrosine hydroxylase protein and quantitation of dopamine and its metabolites by HPLC, dopaminergic cells were revealed in nigral and neostriatal cultures plated on nigral glial cells. No dopaminergic cells were found among neostriatal neurons plated on neostriatal glial cells. It is concluded that glia from substantia nigra but not glia from neostriatum is capable of inducing development or promoting survival of dopaminergic cells.

Animals↗
PubMed1991

The influence of genetic sex on sexual differentiation of diencephalic dopaminergic neurons in vitro and in vivo.

Soma sizes of embryonic male and female tyrosine hydroxylase-immunoreactive neurons were measured in 3 diencephalic regions in situ and in diencephalic dissociated cell cultures. Male neurons were about 30% larger than female neurons both in vitro and in situ. Treatment of cultures with sex steroids did not affect the sex differences. It is concluded that sexual differentiation of dopaminergic neurons may be under primary genetic control.

Animals↗
PubMed1991

[Topography of the organs of the pelvic cavity and macroscopic and histologic findings of the sex organs of a male giant anteater (Myrmecophaga tridactyla) with regard to fertility].

A study was conducted on the reproductive organs of a male Giant Anteater. Discussed is the specific anatomy with its effect on fertility. The ovoid testicles are characterized by an intraabdominal position throughout life. Histologically documented is the active spermiogenesis. The accessory sexual glands consist of a glandula prostatica, glandula vesiculares and glandulae bulbourethrales. The short penis is situated immediately ventral to the anus. The existing anatomic individualities in comparison to other mammalia result in special reproductive aspects and should be taken into consideration for successful breeding.

Animals↗
PubMed1991

Individual olfactory signatures as major determinants of early maternal discrimination in sheep.

Responses of recently parturient ewes to their familiar versus separated (i.e., unfamiliar) twin lambs were observed in an attempt to elucidate further the characteristics of the phenotypic traits (signatures) mediating maternal recognition and bonding. Ewes responded more positively to their familiar lamb than to its twin that had been isolated at birth. Nonetheless, those same mothers also discriminated between their separated twin and unfamiliar alien lambs. Alien lambs elicited similar rejection behavior regardless of whether they had been housed with their own mother or in isolation prior to testing. Lambs appear to have individually distinct (olfactory) signatures; however, the signatures of twin siblings may be sufficiently similar to enable the mother to detect a resemblance. No evidence suggests that acquired maternal labels play a role in either the acceptance of the ewe's own lamb(s) or rejection of aliens.

Animals↗
PubMed1991

Long-term effect of erbium-YAG laser (2.9 microns) on the primate cornea.

An erbium-YAG (2.9 microns) laser was used in the primate eye to irradiate the anterior surface of the cornea in one group and the midstroma in another group after temporary lamellar keratectomy. The eyes were observed clinically up to a period of eight months, when the animals were sacrificed. The initial opacification of the cornea caused by the erbium laser application gradually disappeared over the observation period and was replaced by a faint haze. The healing process was faster in Group II (midstromal laser ablation). Some degree of flattening of the cornea after laser ablation was seen in both groups by photokeratoscopy. At the end of the observation period, the irradiated corneas demonstrated normal appearing corneal structure without significant damage to the corneal epithelium, stroma, or endothelial cells. Further experimental studies are needed to improve the laser delivery system and reduce the degree of corneal damage produced by long laser pulses.

Animals↗
PubMed1991

Vasoactive intestinal polypeptide (VIP) potentiates the behavioral effect of substance P intrathecal administration.

Intrathecal (IT) injection of 20 micrograms substance P (SP) induced a behavioral syndrome consisting of scratching and biting the flanks (83% and 57%, respectively, of 48 rats), and distress-like vocalization (42% of 26 rats tested) in response to a previously innocuous tactile stimulus with a von Frey fiber (allodynia). These behavioral events following SP were of short latency (1-2 min) and duration (around 10 min). Injection IT of 5 micrograms, but not 1 microgram, of vasoactive intestinal polypeptide (VIP), concurrently with SP, significantly increased the frequency of both scratching and biting bouts over that produced by SP alone. VIP IT alone (1 or 5 micrograms) did not stimulate scratching-biting, but induced allodynia in a significant proportion of rats.

Aggression↗
PubMed1991

GABAergic control of receptivity in the female rat.

GABAergic neurotransmission has been implicated in the control of the steroid-dependent behavior, lordosis. GABA has dual effects on lordosis: it facilitates lordosis through actions in the medial hypothalamus (mHYP) and it inhibits lordosis through actions in the preoptic area (POA). In the present study, gonadally intact and ovariectomized female rats were behaviorally tested with a sexually active male. Brains were removed from sexually receptive female either 1 or 24 h after behavioral testing. There was a significant difference in endogenous GABA concentration in HYP and POA between receptive, postreceptive and ovariectomized nonreceptive females. Specifically, GABA levels in postreceptive females were higher in the HYP (20%) and lower in the POA (21%) in comparison to receptive females (p less than 0.05). There was also a significant change in binding parameters of 3H-muscimol in the HYP and POA of receptive females as compared to 24 h postreceptive and ovariectomized rats. Attempts to modulate 3H-GABA release from hypothalamic tissue slices by estrogen or progesterone in ovariectomized rats yielded no effect on this parameter.

Animals↗