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C Biesold

Publications and source records attributed to C Biesold.

9 recordsLinked to original sources
PubMed1998

The presence of chorionic gonadotrophin beta subunit in normal cyclic human endometrium.

The aim of the present study was to determine whether human endometrial cells are able to secrete beta-chorionic gonadotrophin (betaCG). Immunohistochemical studies and in-situ hybridization were performed in order to provide evidence for the occurrence of betaCG in the normal endometrium in 15 patients in the proliferative phase, two patients in the periovulatory phase and 13 patients in the secretory phase. Neither immunohistochemical nor hybridization reactions could be recognized during the proliferative phase. In contrast, both protein and betaCG mRNA were observed in the glandular cells of the endometrium during the secretory phase. The results were supported by Western blotting of secretory phase endometrium extracts and the assessment of the functional secretory capacity of primary endometrium cultures. In comparison with cultured and separated cell fractions, tissue extracts showed a higher betaCG, indicating a regulatory interaction. In conclusion, betaCG can be demonstrated in normal human cyclic endometrium, suggesting a paracrine role in endometrial physiology.

Cells, Cultured↗
PubMed1997

[Immunohistochemical evidence of immuno-reactive hCG in the secretory endometrium in women].

The endometrium is a highly differentiated hormone dependent tissue. Its function is to make an implantation and pregnancy possible. The present study was carried out to investigate whether human endometrial cells are able to produce immune reactivity like hCG (ir-hCG). Immunohistochemical examinations of the endometrium were performed in 15 patients in the proliferative phase, 2 patients in the peri-ovulatory phase and 13 patients in the secretory phase. Immunohistochemical investigations of placenta from women who had an artificial abortion were used as controls. No immunohistochemical reaction in the endometrium could be recognized during the proliferative phase. In contrast a clear reaction was observed in the cells of the epithelium of the endometrium during the secretory phase. At the beginning of the secretory phase the ir-hCG was detected to be in the basal subnuclear region. In the middle of the secretory phase ir-hCG was found in the apical supranuclear region and later on in the glands. In the stroma of the endometrium single immunohistochemical positive cells could be seen. The function of the ir-hCG of the endometrium should be elucidated.

Adult↗
PubMed1995

CD 44 exon v6 as a predictor of lymphatic metastases in cervical carcinoma--an immunocytochemical study of 94 cases.

The relation between expression of several splicing variants of the CD 44 glycoprotein by tumor cells and the increased risk of metastases was discussed recently. By means of an immunocytochemical study (imprint cytology specimens from 94 invasive cervical carcinomas) we have shown a significant correlation between expression of CD 44 v6 and invasion of lymphatic vessels, lymphangiosis carcinomatosa in the primary tumor and the total number of positive pelvic lymph nodes. Expression of CD 44 v6 was not correlated with staging, grading and histological type. CD 44 v6 could therefore be considered as a predictor of lymphatic metastases in cervical carcinoma.

Adenocarcinoma↗
PubMedDate not supplied

Utero-ovarian interaction in the regulation of reproductive function.

The physiological regulation of fertile reproductive cycle in mammals depends on interactions between hypothalamus-pituitary, ovarian and uterine stimuli. Over the past 20 years, much has been learned about the interrelation between the affluent and effluent lymph and vascular drainage in and around both ovarian and uterine tissues. An essential feature in the regulation of the fertile cycle is the functional status of the ovary, particularly the corpus luteum. During the time of implantation and the early pregnancy, an active corpus luteum is essential. As human chorionic gonadotrophin (HCG) is important in the maintenance of the corpus luteum, we investigated if it was produced by the cyclic endometrium. Immunohistochemical and in-situ hybridization reactions were performed but neither identified the presence of HCG during the proliferative phase. Positive staining and beta-human chorionic gonadotrophin (beta-HCG) mRNA were observed during the secretory phase in the glandular cells of the endometrium. The results were confirmed by Western blotting of secretory phase endometrium extracts and assessment of the functional secretory capacity of primary endometrial cultures. Polymerase chain reaction (PCR) investigations showed a positive result in the secretory phase. We postulate that, based on the very close morphological interrelation between the uterus and the ovary, the beta-HCG of the endometrium is the primary factor for the maintenance of the corpus luteum and early pregnancy.

Animals↗