Biomedical subjects
C Bignell
Publications and source records attributed to C Bignell.
Chronic prostatitis/chronic pelvic pain syndrome: national survey of genitourinary medicine clinics.
We sought to determine current practice in the diagnosis and management of chronic prostatitis/chronic pelvic pain syndrome (CPPS) in genitourinary medicine departments in the UK, using a detailed questionnaire survey. Evaluable responses were received from 147 (69%) clinics. Seventy-nine (54%) clinics reported seeing >10 new CPPS patients per year. A broad range of investigations was reported to be used in the diagnosis of CPPS. Whilst 89 (61%) clinics reported using the four-glass test in diagnosis, 46 (32%) reported using the test in >90% of patients with CPPS, and 42 (29%) reported never using the test. In the treatment, doxycycline or ciprofloxacin were reported to be first line treatment by 98% clinics, mostly in 4-6 week courses; however, great variation was recorded in second-line choices and use of non-antibiotic approaches. This survey demonstrates that patients with CPPS are regularly diagnosed and managed in genitourinary clinics in the UK, with wide variations in diagnostic and treatment practices.
The bacterial ParA-ParB partitioning proteins.
A pair of genes designated parA and parB are encoded by many low copy number plasmids and bacterial chromosomes. They work with one or more cis-acting sites termed centromere-like sequences to ensure better than random predivisional partitioning of the DNA molecule that encodes them. The centromere-like sequences nucleate binding of ParB and titrate sufficient protein to create foci, which are easily visible by immuno-fluorescence microscopy. These foci normally follow the plasmid or the chromosomal replication oriC complexes. ParA is a membrane-associated ATPase that is essential for this symmetric movement of the ParB foci. In Bacillus subtilis ParA oscillates from end to end of the cell as does MinD of E. coli, a relative of the ParA family. ParA may facilitate ParB movement along the inner surface of the cytoplasmic membrane to encounter and become tethered to the next replication zone. The ATP-bound form of ParA appears to adopt the conformation needed to drive partition. Hydrolysis to create ParA-ADP or free ParA appears to favour a form that is not located at the pole and binds to DNA rather than the partition complex. Definition of the protein domains needed for interaction with membranes and the conformational changes that occur on interaction with ATP/ADP will provide insights into the partitioning mechanism and possible targets for inhibitors of partitioning.
Antibiotic treatment of gonorrhoea--clinical evidence for choice.
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The eradication of gonorrhoea.
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Improving the sexual health of the nation.
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A double fluorescence method for determining doxorubicin distribution and vascular supply in the mouse kidney.
Freeze-sectioning followed by freeze-drying completely preserves the topographic distribution of soluble substances, with the additional advantage of permitting permanent mounting. This rapid and effective technique was applied to study the distribution of fluorescence of the cytotoxic drug Doxorubicin in microscopic slices from in vivo treated animals. A fluorescent conjugate of different fluorescence, Blankophor-G-Dextran, which remained in the vessels, was used in the same living animals to characterize the vascular supply in the kidney of treated mice in relation to Doxorubicin distribution.