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C Blincoe

Publications and source records attributed to C Blincoe.

At least 19 recordsLinked to original sources
PubMed1993

Computer simulation of normal and pathological copper metabolism in man.

A digital computer simulation of copper metabolism was used to simulate human copper metabolism. The simulation agrees well with the normal data extant. Wilson's disease (hepatolenticular degeneration) and Menkes' disease (steely-hair syndrome) were simulated. Simulation of the unavailability of accumulated liver copper simulated Wilson's disease if it was assumed that the increased urinary excretion was due to induction of an enzymic mechanism for enhanced excretion. This would be consistent with the genetic defect causing only the sequestering of unavailable copper in the liver. Other genetic defects need not be present. Menkes' disease is also a genetic disease affecting the newborn. It was simulated successfully as a defect in absorption of copper from the gastrointestinal tract.

Adult↗
PubMed1988

Isolation and partial characterization of nickel complexes in higher plants.

The essential trace element, nickel, is readily taken up by plants. The biochemical properties of the nickel complex in intrinsically labeled potato and alfalfa were compared and contrasted to ionic nickel. Potato and alfalfa exhibit a similar in vivo nickel complex. The approximate mol wt is 2200-2400 daltons. The complex has a lower polarity than does ionic nickel. The majority of the complex is a cationic species with a minor anionic species. This is confirmed with electrophoresis, ion exchange chromatography and the two nickel complexes' affinity for cationic and anionic resins. Protein does not appear to be associated with either complex.

Chromatography, Ion Exchange↗
PubMed1985

Distribution of plasma magnesium in various vertebrates.

Plasma magnesium in at least five mammalian species (humans, rat, dog, sheep, cattle) is in the form of a complex, separable from ionic magnesium and plasma protein by size exclusion chromatography on Sephadex G-10. Plasma magnesium in three non-mammalian vertebrates (toads, trout, chicken) behaves similarly to ionic magnesium or as a very small magnesium complex on Sephadex G-10.

Animals↗
PubMed1985

Storage stability of some bovine plasma enzymes.

Blood samples taken from domestic or wild ruminant animals typically require transportation to an analytical laboratory. Depending on circumstances, several hours or even a few days may pass between sampling and analysis. Several diagnostic plasma enzymes were measured in bovine blood samples immediately after sampling and after storage under a variety of conditions. Conditions studied included storing whole heparinized blood at 20 C for 6 hours, storage at 4 C for 3 and 5 days, and freezing freshly prepared plasma once and 4 times before analysis. For studies of erythrocyte enzymes, fresh erythrocytes were compared with erythrocytes frozen once, frozen 4 times, and prepared from whole blood stored for one week at 4 C. None of these conditions deteriorated erythrocyte acetylcholinesterase. The serum pseudoacetylcholinesterase and lactate dehydrogenase were not affected by any storage condition used. By contrast, acid phosphatase was significantly decreased by all storage conditions used. Ornithine carbamoyltransferase, alkaline phosphatase, aspartate aminotransferase, and alanine aminotransferase were stable under some of the storage conditions tested.

Acetylcholinesterase↗
PubMed1983

Dietary chromium - forms and availabilities.

The chromium naturally occurring in plants eluted from Sephadex G-25 at approximately 2600 D. Total chromium was quantitated with flameless atomic absorption spectrophotometry. A plant ligand tagged with radioactive chromium both in vivo and in vitro migrated on Sephadex G-25 identically to the naturally occurring chromium compound. The molecular weight of the radioactively tagged chromium compound was 2600 daltons on Sephadex G-25. Similar complexes isolated from plant species were found attached to an organic ligand. The ligand appears to have 2 components, differing in composition by an amine group. This extremely stable (KD = 9 X 10(-5)anionic complex does not contain peptide or deoxyribose units. When alfalfa was exposed to either Cr(III) or Cr(VI), only Cr(III) was isolated in this organic chromium compound. The alfalfa bioreduction system can be saturated, as evidenced by Cr(VI) isolation of ionic in those plant extracts incubated with high levels of Cr(VI) in vitro. The gastrointestinal chromium physiology studies show that the radioactively labelled plant chromium compounds remained intact through the gastrointestinal tract up to the large intestine. Some degradation products were identified in the rat cecum. Approximately 30% of the plant chromium available to the rat was absorbed across the gastrointestinal tract.

Animals↗
PubMed1981

Thyroid homeostasis as studied by computer simulation.

Physico-chemical factors alone may account for much of the thyroid gland homeostasis and by inference for homeostasis of basal energy metabolism in mammals. Computer simulations indicate that changes of several of the important rate constants for thyroid function have little effect on circulating thyroid hormone concentrations. This was especially true of the rate constants for thyroid hormone release from the thyroid gland and to a lesser extent of the rate constants governing thyroid hormone metabolism and excretion. The rate at which thyroid hormone levels in blood respond to changes was rather slow indicating that transient changes in dietary iodine or in the rate constants governing thyroid function would have little effect. If changes taking place over several days, such as during the initiation of lactation, the parameters of thyroid function change at a corresponding rate.

Animals↗