PubMed Health⌕ Search

Biomedical subjects

C Blow

Publications and source records attributed to C Blow.

7 recordsLinked to original sources

Evaluation of fluorescence excitation transfer immunoassay for measurement of specific proteins.

Fluorescence excitation transfer immunoassay is a suitable technique for the measurement of serum IgG and CRP. The reagents, once reconstituted, are stable for at least 3 months. The method shows no interference due to bilirubin, lipaemia or haemolysis up to high levels. The assay is simple to perform, reliable and offers considerable advantages over manual techniques such as radial immunodiffusion or electroimmunoassay.

C-Reactive Protein↗

Formulation of optimal conditions for an immunonephelometric assay.

A method is described for establishing the optimal conditions for an immunonephelometric protein assay. This method has been derived for use on the Hyland PDQ (Travenol Laboratories, Thetford, UK) and Kallestad (Kallestad Laboratories Inc, Chaska, Mn, USA) nephelometers. We have also found it applicable to the Behring nephelometer (Hoechst, London, UK). Using this approach, it is possible to establish an assay on these instruments for any serum protein with a concentration above about 10 mg/l for which a suitable antiserum is available. This method has also proved suitable for developing methods for proteins in urine, CSF, saliva, and amniotic fluid. Proteins in urine may be measured at concentrations as low as 1 mg/l.

Antigen-Antibody Reactions↗