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Biomedical subjects

C Broelsch

Publications and source records attributed to C Broelsch.

22 records · Page 2Linked to original sources
PubMed1980

Detection and partial characterization of glucocorticoid-binding components in hepatic metastases of human bronchial and gastric carcinoid tumors.

Metastases of human carcinoid tumors of bronchial and gastric origin have been analysed for glucocorticoid receptors using the fluorinated compound [3H] dexamethasone. In both tumors high concentrations of glucocorticoid-binding components were detected which based on several physicochemical characteristics were identified as glucocorticoid receptors in nature. The apparent dissociation constant of the dexamethasone-binder complex was found to be 1.8 x 10(-8) mol/l for the bronchial and 2.8 x 10(-8) mol/l for the gastric carcinoid tumor. Sedimentation in sucrose gradients disclosed the binding components to be macromolecules, sedimenting at about 7.S. By agar gel electrophoresis the molecular species binding dexamethasone were found to exhibit the same electrophoretic mobility as glucocorticoid receptors from well-known target organs. The ligand specificity for binding to these components indicated a requirement of active glucocorticoids as well as anti-glucocorticoids. A regulatory function of glucocorticoids in serotonin biosyntheses is discussed, which may be of potential clinical significance.

Binding, Competitive↗
PubMed1976

Preparation of parenchymal and non-parenchymal cells from adult human liver--morphological and biochemical characteristics.

By perfusion of the isolated human liver with collagenase and hyaluronidase a mixed suspension of various cell types was obtained. Pure parenchymal cells were prepared by differential centrifugation, pure non-parenchymal cells by the use of pronase and subsequent isopycnic centrifugation on metrizamide gradients (50-300 g/l). About 90% of the parenchymal and non-parenchymal cells were viable as judged by trypan blue staining. Non-parenchymal cells were not capable fo gluconeogenesis but at high rates. Parenchymal cells retained their ability to form glucose and to accumulate glycogen from fructose greater than lactate/pyruvate greater than alanine. Studies on binding of 125I-labelled insulin by isolated parenchymal cells were performed at 30 degrees C. The binding data may fit with a minimum of two classes of binding sites: (a) high affinity--low capacity sties (Kd approximately 6.6 nmol/l, capacity approximately 16 000 insulin molecules per cell) and (b) low affinity-high capacity sites (Kd approsimately 0.37 mumol/l, capacity approximately 646 000 molecules per cell).

Adult↗
PubMedDate not supplied

Oxygen supply to the cirrhotic liver following various portacaval shunt procedures.

Direct measurements of local oxygen pressure by means of a platinum multiwire electrode were performed to investigate the effect of five different portacaval shunt procedures on hepatic oxygen content in the cirrhotic rat liver. End-to-side shunt, side-to-side shunt, mesenterico caval shunt, splenocaval shunt, and portacaval transposition were performed and surface PO2 was determined immediately after operation, 24 hours following operation and after one week. Portacaval transposition and end-to-side shunt led to a striking oxygen deficit of the liver tissue with no incidence of compensation by the hepatic artery; Oxygen supply was improved considerably by a side-to-side shunt and tissue hypoxia could be prevented by a mesenterico-caval shunt and splenocaval shunt. This improving effect is thought to be due to the pancreatico-duodenal venous blood supply which should be carefully preserved for the liver circulation when a shunt needs to be performed.

Animals↗