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Biomedical subjects

C Bruno

Publications and source records attributed to C Bruno.

At least 109 records · Page 6Linked to original sources
PubMed1991

[Progression of multiple innervation of reinnervated rat muscle treated with bilobalide].

During motor nerve regeneration a transitory polyinnervation of muscle cells occurs, which represents a phase of rearrangement of the recovered innervation. Bilobalide, a terpene extrated from Ginkgo biloba leaves, was proposed to affect some aspects of nervous system development and regeneration. In this work, influence of bilobalide on polyinnervation in reinnervated extensor digitorum longus muscle was studied, through electrophysiological and histological techniques. The muscle was denervated crushing the sciatic nerve and it was examined at 1 or 2 months after denervation. The polyinnervated muscle cells in controls reached 24% at 1 month and thus the percentage decreased. In muscles of bilobalide treated rats the number of polyinnervated cells was decreased at both times.

Action Potentials↗
PubMed1990

Maturation of the spontaneous transmitter release by regenerated nerve endings in vitamin E-deficient rats.

In order to verify the importance of the protection against lipid peroxidation in presynaptic differentiation and maturation, the reappearance and maturation of the spontaneous transmitter release during the extensor digitorum longus muscle reinnervation following a lesion of the sciatic nerve were studied in normal and vitamin E-deficient rats. The study was carried out by intracellular recordings in order to observe the miniature end plate potentials in the reinnervated end plates. In control and vitamin E-deficient rats the first signs of muscle innervation reappeared simultaneously, but in the latter the spontaneous transmitter release mechanism matured more slowly; furthermore, in the long-term, very low mepp frequencies continued to occur. The data suggest a slowing of the transmitter release mechanism maturation and a protracted rearrangement of innervation in the deficient rats.

Animals↗
PubMed1990

Lysis protein S of phage lambda functions in Saccharomyces cerevisiae.

The lambda S lysis gene was cloned into a Saccharomyces cerevisiae expression vector under GAL1 control. Induction with galactose in S. cerevisiae terminated cell growth and prevented colony formation. Several membrane proteins immunoreactive with anti-S antibody accumulated in the membranes, indicating that sodium dodecyl sulfate-resistant oligomers of S are formed, similar to those observed in the membranes of Escherichia coli cells killed by expression of the S gene. These observations suggest that the S gene product functions as a cytotoxic protein in the yeast cytoplasmic membrane as it does in the bacterial membrane.

Amino Acid Sequence↗
PubMed1990

[Mortality in malignant tumors of the peritoneum in Italy: search for correlations with exposure to asbestos].

In the years 1981-85, 2438 malignant neoplasms of peritoneum occurred in Italy: the crude mortality rate was 0.86 X 10(-5) per year. The standardized mortality rates of Italian provinces ranged from 0.20 to 1.86 X 10(-5) per year. Some degree of misclassification can affect the ascertainment of peritoneal tumors as a death cause. In some of the Provinces with the highest mortality rates dockyards or asbestos-cement industries are located (e.g. Genova, La Spezia; Alessandria); in other Provinces the presence of industries involving an important use of asbestos could not be ruled out.

Adult↗
PubMed1988

Ashley's hypothesis revisited.

Nowadays it is widely accepted that cervical cancer is a virus related, sexually transmitted disease, but no complete agreement exists about its etiological factors. They may be identified in HSV or in HPV or in both. Moreover, its is accepted that cervical cancer does not arises ex abrupto from normal epithelium, but often arises from the epithelia with well codified morphological lesions now defined as "Cytohistological Cervical Cancer Precursors". Epidemiological data available at the moment, also concerning risk age, suggest that two different risk classes do exist. They range respectively between 20 and 40 years and between 50 and 70 years of age. When considering sexual promiscuity, younger patients refer a higher and more intensive level than that referred by older; the latter often claim for a low level of sexual activity. Starting from this observation, as well as from clinical and histological evidence, Ashley first suggested in 1965 the existence of two different types of cervical cancer. In the Ashley hypothesis the first type, slow-growing, is often preceded by a precursor, non-invasive stage, while the second, fast-growing, rapidly invades, often without evidence of a previnvasive stage. In our series of 1,010 invasive and 210 in situ cervical cancers, indeed, two different ranges of risk-ages exist, fulfilling Ashley's hypothesis. Morphological evaluation of lesions shows that in the younger patients, invasive cancer is, in a high percentage, associated with HPV-VCE and CIN in the surroundings, while in the older, this feature is less evident.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗
PubMed1986

Effect of ethanol on the spontaneous transmitter release by nerve endings in the process of maturation.

Ethanol stimulates the spontaneous transmitter release from motor nerve endings, as shown by the increase of miniature end plate potential (m.e.p.p.) frequency at the neuro-muscular junction. The stimulation of acetylcholine spontaneous quantal release by ethanol is greater in regenerating than in mature nerve endings. The different effects of ethanol on regenerating nerve endings may be related to changes of chemical-physical membrane properties.

Animals↗
PubMed1985

[Effect of ethanol on young nerve endings].

The ethanol changes the quantal spontaneous release of acetylcholine and it affects the reinnervation time course. The effects of ethanol on regenerated nerve endings have been tested. 20 days after crushing sciatic nerve, the m.e.p.p. frequency at the end plate of rat extensor digitorum longus muscle keep in Ringer solution without and with ethanol has been estimated by intracellular recordings. The increase of the m.e.p.p. frequency produced by ethanol is greater in immature, than in normal nerve endings.

Animals↗
PubMed1985

[Recovery of sensation and reflex activity during treatment with ethanol].

Ethanol greatly modifies synaptic function and it affects the reinnervation time course. In order to better clarify the effects of ethanol on nerve regeneration, we have observed the recovery of algesthesia and reflex activity in the rat during the treatment with ethanol. For this purpose the posterior leg of Sprague Dawley rats was denervated by crushing the sciatic nerve and the recovery of algesthesia and reflex activity was tested at the level of the metatarsus-falanx articulation of the homolateral paw. During the reinnervation period, the animals were treated daily with ethanol 3 g/Kg of body weight. The recovery of algesthesia and reflex activity comes about in a medio-lateral direction and proceeds linearly. It is completed on the 25th day after the denervation. Ethanol does not cause changes in the time course of algesthesia recovery, however it does cause a slight delay in the recovery of the reflex activity.

Animals↗