PubMed Health⌕ Search

Biomedical subjects

C Bueno

Publications and source records attributed to C Bueno.

At least 19 recordsLinked to original sources
PubMed1990

Antiphospholipid antibodies in syphilis.

1. An enzyme-linked immunosorbent assay was used to determine the phospholipid specificity of antibodies present in sera from 35 syphilis patients. 2. Based on the cross-reaction obtained against a mixture of cardiolipin, phosphatidylcholine and cholesterol that is standard for flocculation tests according to the Venereal Disease Research Laboratory (CECON, São Paulo, Brazil), all 35 patients tested positive for antibodies of the IgG class whereas 13 (37%) also had IgM antibodies for the same mixture of lipids. IgG antibodies to cardiolipin were demonstrated in 2 patients (6%) and IgM antibodies in 5 (15%). Significant levels of IgG anti-phosphatidylcholine were detected in 3 patients (9%) and IgM antibodies in 4 (11%). IgG anti-phosphatidylethanolamine antibodies were found in 1 patient (3%) and IgM antibodies in 3 (9%). Antibody binding to cardiolipin plus cholesterol or cardiolipin plus phosphatidylcholine was as effective as when the standard mixture of all 3 lipids was used. 3. A comparison with serum from systemic lupus erythematosus patients and inhibition studies using liposomes of cardiolipin or the mixture of 3 lipids suggests that there are at least 3 groups of anticardiolipin antibodies.

Antibodies, Anti-Idiotypic↗
PubMed1989

Anticardiolipin antibodies in Behçet's syndrome: a predictor of a more severe disease.

A high incidence of anticardiolipin antibodies were detected in 7 of 20 patients (35%) with Behçet's Syndrome. Three patients had IgG-ab, three had IgM-ab and one had both IgG and IgM antibodies. IgG-ACA was detected mainly in patients with ocular disease (30%) and one of them also has cerebral vascular disease. A lower incidence of ACA was found in the patients taking steroids compared with the ones taking other drugs. This work draws attention to the more severe disease present in patients with ACA and also the possibility of such tests become negative in patients taking immunosuppressive drugs.

Adult↗
PubMed1989

Anticardiolipin antibodies in patients with rheumatoid arthritis.

Anticardiolipin antibodies (ACA) were assayed by ELISA in 73 patients with rheumatoid arthritis. Twelve (16.48%) patients showed levels of ACA three standard deviations above the value of the control group and were considered positive; these patients were compared to the group with ACA within the normal levels regarding the following clinical and laboratorial characteristics: spontaneous abortions, central nervous system involvement, systematization and activity of disease, alterations in platelet counts, presence of antinuclear antibodies and rheumatoid factor. Significant statistical association could be demonstrated between systematization and presence of antinuclear antibodies (ANA) and positiveness to ACA (IgG, IgM or both). These findings might indicate that ACA in patients with RA could have relevance to morbidity of disease or perhaps to its pathogenesis.

Adult↗
PubMed1988

Rheumatoid factor in rabbits with antigen-induced arthritis.

Rheumatoid factor was assayed weekly by hemagglutination in sera from rabbits with antigen-induced arthritis and from control immunized animals receiving intra-articular injections of saline. Both groups developed rheumatoid factor. Arthritic animals, however, maintained high titers of rheumatoid factor more persistently than controls. Pre-incubation of positive sera with native or aggregated rabbit IgG was unable to inhibit agglutination, while native and aggregated human IgG were highly effective. Rheumatoid factor was unable to induce arthritis and probably does not potentiate articular tissue damage by immunocomplexes. Although production of rheumatoid factor may be a consequence of the polyclonal activation promoted by the immunization with complete Freund's adjuvant (CFA), the presence of arthritis is associated with persistence of high titers of rheumatoid factor for longer than would be expected from immunization with CFA alone.

Animals↗
PubMed1988

Antibodies to collagen in experimental antigen induced arthritis of the rabbit.

Fifteen rabbits with antigen (bovine serum albumin) induced arthritis had antibodies to collagens type I, II and III detected weekly by passive hemagglutination. Antitype I collagen antibodies were detected in 80% of the animals in the 3rd week of arthritis; antitypes II and III were found less frequently. No anticollagen antibody was detected after the 6th week of arthritis. Although the appearance of these antibodies was clearly related to the induction of arthritis, results indicate that humoral immunity to collagen is unable to initiate or contribute to the perpetuation of synovitis in this experimental model. Antibodies to collagen are probably an epiphenomenon of articular damage in the antigen induced arthritis of the rabbit.

Animals↗
PubMed1986

Enzyme-linked immunosorbent assay for detection of antibodies to extractable muscle antigens in myasthenia gravis.

A purified citric acid soluble extract from human skeletal muscle (AEMA) and a phosphate-buffered saline extract from rabbit muscle acetone powder (EMA) were used to coat polystyrene beads in an enzyme-linked immunosorbent assay (ELISA). From 54 patients with myasthenia gravis, positive results were observed in 14. Five of 6 patients with thymoma had high levels of antibodies. With the diagnostic difficulties in detecting small and medium-sized thymoma, a sensitive assay for detection of antibodies to muscle antigen may be an important supplementary tool to detect tumors at early stages.

Animals↗
PubMed1979

[Multiple clear cell acanthoma in a patient with psoriasis. Treatment with 5-fluorouracil].

Clear cell acanthoma (CCA) is a benign skin tumour, seldom multiple, perfectly individualized both clinically and histologically. The tumour is more usually mistaken for a seborrheic keratosis. There are large amounts of glycogen within the epidermal cells as can see upon staining with the PAS reaction. Melanogenesis is decreased. The enzymes characteristic of eccrine sweat glands are also decreased. The authors report a 67 years old psoriatic female with 15 C.C.A. They emphasize the treatment with topic 5FU particularly interesting in the multiple forms.

Administration, Topical↗
PubMedDate not supplied

Epidermal nuclear immunoglobulin deposition in connective tissue diseases.

Epidermal nuclear deposition of immunoglobulins (in vivo ANA) was observed in 45 out of 252 skin biopsies (17.8%). It occurred in 19% of cases with systemic lupus erythematosus, in 32% of the mixed connective tissue disease, in 22% of the scleroderma, in 20% of the cutaneous vasculitis, in 18% of the polymyositis, in 33% of the Sjogren's syndrome, but it was absent in cases with rheumatoid arthritis. The in vivo ANA showed a significant association with serum antibodies to an extractable nuclear antigen (ENA), with speckled pattern of immunofluorescent antinuclear antibody (FANA) and with antibody to a fraction of ENA sensitive to ribonuclease termed ribonucleoprotein (RNP). Indirect evidence was obtained suggesting that the epidermal nuclear deposition of immunoglobulins is a true in vivo phenomenon: some patients with serum antibodies to ENA do not display in vivo ANA and contrariwise, no difference was detected between diseased and normal skin for the occurrence of in vivo ANA and also no association was observed between this phenomenon with immune deposits at dermoepidermal junction or in subepidermal vessels.

Antibodies, Antinuclear↗
PubMedDate not supplied

Anticardiolipin antibodies and disease activity in systemic lupus erythematosus.

We studied a group of 80 unselected patients with systemic lupus erythematosus (SLE). Twenty six (32.5%) of them were considered in clinical activity using criteria based on clinical features and laboratory abnormalities. Using an isotype specific enzyme linked immunosorbent assay (ELISA) for anticardiolipin antibodies we found IgG anticardiolipin in 28/80 (35%) and IgM anticardiolipin in 20/80 (25%) patients. Antibodies to native DNA by immunofluorescence were found in 11/80 (14%) patients, whereas antibodies to denatured DNA by ELISA were found in 51/80 (64%) patients. There was an association between disease activity and the presence of anticardiolipin and anti-DNA antibodies particularly of the IgG isotype. We also found an association between anticardiolipin and anti-DNA suggesting a cross reaction between these antibodies, but we are not able to exclude the possibility of a simple coincidental phenomenon due to polyclonal synthesis of immunoglobulin observed during periods of disease activity.

Adolescent↗
PubMedDate not supplied

[Clinical and biochemical characterization of isoniazid-induced auto-antibodies].

Isoniazid (INH) is one among many drugs capable of inducing autoantibodies and, in some cases, a lupus-like syndrome (LE). A longitudinal study was performed in 24 tuberculosis patients treated with INH to detect antibodies (A-AH) to total histones and fractions. Antinuclear antibodies were observed in two patients after treatment. Higher frequency of IgM-AH was also observed. IgM-AH binding to all fractions were observed in those serum samples exhibiting stronger ELISA reactivity. Conversely, binding to only H1 occurred when lower IgM-AH activity was tested. Correlations with clinical expressions of LE were not observed in the present study.

Adolescent↗
PubMedDate not supplied

Association of anti-DNA and anticardiolipin antibodies in systemic lupus erythematosus.

Recent studies have shown that anti-DNA and anticardiolipin antibodies in patients with SLE may cross-react. Using an ELISA for determination of these antibodies, a strong association between IgG anti-denatured DNA and IgG anticardiolipin antibodies was found. Eight sera samples with the highest levels of both antibodies were selected to determine the possibility of a cross-reaction. The levels of anticardiolipin were not affected by denatured DNA in concentrations adequate to inhibit the anti-DNA binding. These data did not confirm previous studies using monoclonal antibodies showing cross reactivity between the two groups of antibodies. Nevertheless, a population of antibodies that may cross-reacts, in some special cases, cannot be ruled out.

Antibodies, Antinuclear↗