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C Bugnon

Publications and source records attributed to C Bugnon.

At least 91 records · Page 5Linked to original sources
PubMed1976

[Cyto-immunologic location of alpha-MSH and ACTH in the lead-hematoxylin (HPb)-stainable pituitary cells, in the eel].

The lead-haematoxylin positive cells of the pars intermedia react with anti-alpha-MSH and anti-1-24ACTH or anti-17-39ATCH; those of the rostal pars distalis are only revealed with antisera anti-1-24ACTH and anti-17-39ACTH. Intensity of cytoimmunological staining, which is modified after various experimental treatments (reserpine, metyrapon, pimozid, cortisol...) and during black or white background adaptaiton, corresponds essentially to that of the PbH staining.

Adrenocorticotropic Hormone↗
PubMed1976

[Cytoimmunological study of the corticotrophic and corticomelanotrophic cells in the adenohypophysis of Boops salpa L. (marine teleost) in different experimental conditions (variation of salinity and of background color)].

The cytoimmunological study of the adenohypophysis of Boops salpa (marine Teleost), in different experimental conditions confirmed that the MacConaill's hematoxyline-positive cells contain: 1. ACTH (Corticotrophic Hormone) in the pars distalis, 2. MSH (Melanocyte-Stimulating Hormone) and ACTH in the pars intermedia.

Adaptation, Physiological↗
PubMed1976

[Cytoimmunological demonstration of LH-RH neurons in the human fetus].

Neurons and axons were characterized by means of an antiserum against LH-RH in hypothalamus of human faetuses from the 13th week of gestation until birth. The morphology and the elective localization of neurons and axons in the mediobasal hypothalamus, premamillary area and in lamina terminalis, and the termination of axons, particularly those of the tubero-infundibular tract, are described. The results obtained permit discussion of hypothalamic control of gondotropic secretion in the human faetus from the 13th week.

Axons↗
PubMed1976

[States of fragility of chromosome 7 in man].

The examination of the chromosomes of a seven year old child with severe mental retardation, allowed us to bring to light, beside a majority of cells with a normal karyotype, a population of about 10% of cells in which chromosome 7 showed a gap in the juxtacentromeric area of its short arm. The degree of this alteration varied from the simple angulation to the complete break with short arm's loss. The functional and structural signification of this abnormality is discussed and this case is compared with previous observations concerning the abnormalities of this area of chromosome 7 (specially the causal aberrations occuring in cell cultures).

Child↗
PubMed1976

[Cyto-immunological study of the anterior pituitary somatotropin cells of male and female adult Peking, Barbary and hybrid (from crossing male Peking X female Barbary) ducks and of male ducklings of the 3 races)].

The anti-human-STH immunserum applied on hypophysis sections of adults males and females Peking (Anas platyrhynchos L.) Barbary (Cairina moschata L.) and Hybrid (from the mating male Peking X female Barbary) ducks, marks orangeophils, oval- or round shaped, PAS negative cells exclusively localized in the caudal lobe of the pars distalis. In the males ducklings of the three races this immunserum reveals numerous and partially exhausted cells. The authors conclude that the acidophils alpha cells observed in the caudal lobe of the duck are somatotrophic cells.

Age Factors↗
PubMed1976

[Comparative study in the guinea pig, cat and dog of hypothalamo-infundibular nervous fibers immunoreactive to an anti-LH-RH immune serum].

In the median eminence of guinea pig, cat and dog some axons of the hypothalamo-infundibular tract are immunoreactive with anti LH-RH. In these three species, the axons have a similar topography and area of peripheric terminations. These one occur at the capillaries of Mantelplexus. It was also shown, in the cat, that these areas of termination exhibit a strong monoamine fluorescence.

Animals↗
PubMed1976

[Immunocytochemical study using semi-thin sections of the phenomena of early differentiation in several cell populations of the human fetal adenohypophysis].

Immunocytological investigations have been performed on semi-thin sections of human fetal pituitaries ranging in fetal age from 6 to 26 weeks. Corticotrophs can be revealed by anti-ACTH (1-24), anti-ACTH (17-39) and anti-beta MSH but not by anti-alpha MSH immunesera from the 8th week. Somatotrophs are revealed with anti-human STH from 9th week. Differentiating cells containing only alpha subunits of glycoproteic hormones are present from 8th to 12th week. At 13th week beta subunits of TSH can be revealed immunocytologically in thyreotrophs. Beta subunits of LH or FSH can be detected in same gonadotrophic cells only from 15th week.

Adrenocorticotropic Hormone↗
PubMed1976

[Cyto-immunological study of the Pleurodele pituitary gland].

In the pituitary of an Amphibian (Urodela), various cell types were localized by cytoimmunological techniques: corticotrophs located in a rostral zone near the median eminence, ventral orangeophilic cells secreting a prolactin-like hormone and erythrosinophilic cells in a more dorso-caudal situation secreting growth hormone. Alpha-MSH and beta-MSH produceng cell were identified in the intermediate lobe; these cells were also labelled with antibodies against 1-24 ACTH and 17-39 ACTH. These data are in accordance with results obtained after an experimental study of the prolactin cells.

Adrenocorticotropic Hormone↗
PubMed1976

[Cyto-immunological studies of the gonadotropic cells of the human fetus].

The immunicytological analysis of PAE fixed semi-thin sections of pituitary cells (of human fetuses aged 6 to 26 weeks) using immunesera directed against the human total glycoproteic hormones, HCG, LH, FSH and TSH or made specific for the LH, FSH, and TSH beta subunits shows: -- the presence up to the 12th week of only the subunit of the glycoproteic hormones revealed indifférently by our anti total hormones immunesera; -- the apparition of the betaTSH subunits at the 13th week, characterizing the differentiation of the thyrotrophs; -- the apparition of betaLH and betaFSH subunits on the one and same gonadotropic cell population at the 15th week.

Animals↗
PubMed1976

[Immuno-cytologic study of LH-RH cells and fibers in the human fetus].

The application of immunofluorescence and immunoenzymology techniques with an anti LH RH I.S. permitted to reveal neurons and fibers in the human fetal hypothalamus from the 13th week up to birth. The morphology and dispersion areas of the neurons in the mediobassal, premammillary and anterior hypothalamus (lamina terminalis and its surroundings and the septum) as well as the direction and the destination of the immuno-reactive fibers of the hypothalamo infundibular tract have been described. These fibers are in contact with the capillaries of the Mantelplexus before and after the apparition of the intra-eminential loops at the 16th week. The general topography of the LH RH peptidergic system in the human fetus can be compared with the topography of other adult primates. The role of this system in the maturation and the control of gonadotropic cells is evoked.

Female↗
PubMed1975

Immunocytochemistry of pituitary corticotroph cells in terrestrial turtle (Testudo mauritanica).

The immunocytochemical and histochemical characters of the corticotroph cells of the Turtle adenohypophysis have been studied. These cells are localised in the rostral part of the gland and are revealed by Is anti ACTH (1-24) and (17-39). They are also colored with lead hematoxyline and PAS-Orange G. The corticotroph nature of these cells is confirmed by the study of their modifications after treatment with amphenone and ACTH. The Is anti ACTH also reveal most of the cells of the pars intermedia; while the Is anti beta-MSH reveals only these cells and some scatter cells of the pars distalis.

Adrenocorticotropic Hormone↗
PubMed1975

[Immunological study of gonadotropic and prolactin cells in adenohypophysis of the Barbary duck (Cairina moschata L.)].

In both lobes of the pars distalis of the Barbary duck the basophile, alcian-blue positive and PAS negative cells no affected by thyroxine treatment are revealed by anti-HCG-immunserum; they are certainly gonadotropic cells previously named delta-cells in the Pekin duck. In the cephalic lobe only one type of PAS positive cells, is revealed by antiprolactin antiserum; they are abundant and very active in the broody duck.

Animals↗
PubMed1975

[Effect of various inhibitors on oxidative activity and ultrastructure of rat liver mitochondria].

Parallel studies concerning the influence of some inhibitors on electron transfer, and ultrastructural aspects of rat liver mitochondria led to the conclusion that metabolic activity and mitochondrial ultrastructure are closely linked ; when mitochondria oxidize succinate, their configuration change from the "condensed" to the "orthodox" state ; this change does not occur if the oxidation is inhibited.

Animals↗
PubMed1975

[Cytoimmunological and histochemical study of cat adenohypophyseal cells, made fluorescent by the Falck and Hillarp technic].

In the Cat, after Falck and Hillarp method, all the fluorescent cells of the PI and the anterior lobe of adenohypophysis can be revealed with specific anti-sera to ACTH(1-24), ACTH(17-39), bovine beta-MSH and porcine beta-LPH. With the lead hematoxyline staining method, two types of cells are recognizable in the anterior lobe, in which the non hormonal constituents of the granules must be different.

Adrenocorticotropic Hormone↗
PubMed1975

[Corticotrope and melanotrope cells in cat, fox, rat and human fetus adenohypophysis: studies with induced fluorescence, cytoimmunologic technics and lead hematoxyline].

Formaldehyde-induced fluorescence, acid-catalyzed or not, methods, and immunocytology with anti-ACTH (1-24), anti-ACTH(17-39), ANTI-BETA-LPH immunserums were applied on the same preparations of cat, fox, rat and human foetus pituitaries. The superpositions of results showed that the pars intermedia and pars distalis corticomelano-lipotrophic cells of fox and cat pituitary, those of human foetal pituitary, and the purely corticotrophics cells of the rat pars distalis contained a fluorogenic probably granular compound. Moreover, the granules of the same cell types were electively revealed on our lymphilized material by plombic hematoxylin. Only the anti-beta-LPH and/or anti-beta-MSH fixing celpls exhibited hypercyanophilic, PAS-positive and bleu alcian-positive caracteristics.

Adrenal Glands↗
PubMed1975

[Cytoimmunological detection of corticotropic and melanotropic cells in the human fetal adenohypophysis in early stages of development].

The corticotropic and melanotropic cells of eight human adenohypophyses from eight to twelve weeks-old foetuses were identified with immunofluorescent and immunoenzymatic procedures. Anti-ACTH (1-24), anti-ACTH (17-39), anti-beta-LPH and anti-beta-MSH immunsera were electively fixed by the same cell type. These cortico-melanotropic cells, localized on the edge of glandular cords in contact with vascular mesenchym, both in the anterior and posterior walls of Rathke's pouch, were PAS-positive, cyanophilic, and plombic hematoxylin positive.

Adrenocorticotropic Hormone↗