Biomedical subjects

C C Chase

Publications and source records attributed to C C Chase.

72 records · Page 4Linked to original sources

PubMed1993

Effect of dietary energy on growth and reproductive characteristics of Angus and Senepol bulls during summer in Florida.

Pubertal Angus bulls (n=10, 503 days of age and weighing 366 kg) and Senepol bulls (n=10, 457 days of age and weighing 381 kg) were stratified by age and weight into 2 dietary treatments formulated to provide equal amounts of crude protein and 75% (below) or 150% (above) of the maintenance requirements for metabolizable energy. Measurements to assess body growth and libido were collected at 28-day intervals for 112 days (June through September). Twice during each 28-day interval, the bulls were subjected to breeding soundness examinations. At the end of the experiment, gonadotropin releasing hormone (GnRH) - induced secretion of luteinizing hormone (LH) and testosterone (T) in the serum were determined. At the end of the experiment, bulls fed the above maintenance diet (P<0.0001) were 91 kg heavier, had 1.7 mm more backfat thickness and 12.6 cm(2) larger ribeye area than bulls on a below maintenance diet. Diet affected (P<0.003) the average daily change in scrotal circumference, but not the libido score (P>0.1) or semen quality. In general, Angus bulls had superior initial semen quality (P<0.06); however, during summer, semen quality tended to decrease in Angus but not in Senepol bulls. The final rectal temperature was 0.5 degrees C lower (P<0.003) in Senepol than in Angus bulls. Basal T concentrations and area under the GnRH-induced T curve were greater (P<0.07) for bulls fed the above rather than the below maintenance diet. Angus bulls had a higher (P<0.03) maximal LH response to GnRH and larger area under the GnRH-induced LH curve than Senepol bulls.

Journal Article↗

PubMed1993

The effect of gossypol in cottonseed meal on performance and on hematological and semen traits in postpubertal Brahman bulls.

The effects of a cottonseed meal diet containing high levels of free gossypol on hematological traits, including erythrocyte osmotic fragility and semen characteristics, were examined during an 11-week period. Eight Brahman bulls were randomly assigned to 1 of 2 groups. The control group (n=4) was fed a mixture of soybean meal and corn. The treated group (n=4) was fed a mixture of cottonseed meal and corn. Both groups were allowed hay free choice. The treated group consumed 8.2 g of free gossypol per bull per day. The percentage of normal spermatozoa was lower (P<0.01) in the treated than in the control group from Week 5 (49+/-9.8 vs 83+/-3.2%), which was primarily influenced by changes in mid piece morphology in the treated bulls. Erythrocyte osmotic fragility was higher (P<0.001) in the treated than in the control group over the entire study period, although group values diverged more acutely from Week 7 of treatment. Sperm motility was lower (P=0.04) in treated bulls than in control bulls at Week 9 (52+/-9.8 vs 82+/-6.2%). These data suggest that concurrent discernable changes in bull erythrocyte osmotic fragility and in semen characteristics can occur following commencement of a diet containing 8.2g of free gossypol per day.

Journal Article↗

PubMed1993

Nested and multiplex polymerase chain reactions for the identification of bluetongue virus infection in the biting midge, Culicoides variipennis.

Two polymerase chain reaction tests for the detection of bluetongue viral (BLU) RNA in the principal North American insect vector, Culicoides variipennis, were developed. The BLU serogroup specific test used the highly expressed non-structural protein 1 gene as the target gene and two amplification steps. First a 1228 base pair product was amplified using an outer primer pair, then a second amplification using a nested or internal primer pair produced a 930 base pair product. This nested PCR test was found to be very sensitive detecting an equivalent to 1 plaque-forming unit of BLU viral RNA extracted from infected biting midges. The serotype specific test used a multiplex PCR approach in which five different primer pairs were used simultaneously. Each pair was based on the variable outer capsid protein VP2 gene of the five US serotypes generating specific product which were easily identified by size difference. The sensitivity of the multiplex PCR was less sensitive than the nested-PCR but sufficient for use with field collected samples. These tests provide valuable tools for epidemiologic studies of BLU disease.

Animals↗

PubMed1992

Effects of exogenous glucose or colostrum on body temperature, plasma glucose, and serum insulin in cold-stressed, newborn Brahman calves.

The effects of ambient temperature and source of exogenous energy (glucose or colostrum) on the ability of newborn Brahman calves to maintain rectal temperature (RT) were determined. All calves were removed from dams within 30 min of birth, before suckling. Calves were catheterized and placed in either a warm (25 degrees C) or cold (5 degrees C) environment for 150 min and given either colostrum or glucose. This resulted in four groups (warm colostrum, n = 7; cold colostrum, n = 7; warm glucose, n = 6; cold glucose, n = 6). Blood samples and RT were obtained at 15-min intervals during warm or cold through 150 min, when calves were removed from cold, and at 180, 240, and 300 min. After 60 min, each calf was given either 1 L of colostrum (38 degrees C) from its dam or glucose (38 degrees C) infusion of 750 mg/kg BW. Plasma glucose concentrations were determined by enzymatic techniques and serum insulin concentrations by RIA. Calves exposed to cold or warm air temperatures had similar declines in rectal temperature from 0 to 60 min. Colostrum-fed, cold calves had a greater (P less than .07) decrease in RT than did colostrum-fed, warm calves from 75 through 150 min; glucose-infused warm and cold calves had intermediate decreases in RT. Plasma glucose increased (P less than .0001) in glucose-infused compared with colostrum-fed calves at 75 min, but glucose-infused calves had lower (P less than .02) glucose levels from 180 to 300 min. Higher (P less than .05) glucose concentrations

Animals↗

PubMed1992

In vitro metabolism of glucose by bovine reproductive tissues obtained during the estrous cycle and after calving.

Two experiments were conducted to determine the effect of physiological state (stage of the estrous cycle or time after calving) on the in vitro metabolism of glucose by reproductive tissues. In Exp. 1, the corpus luteum and ipsilateral uterine horn were collected at surgery from 15 cows at early (d 6 to 7), middle (d 10 to 13), or late (d 17 to 19) stages of the estrous cycle. Luteal or endometrial tissues (45 mg) were incubated (4 h, 37 degrees C) in metabolic flasks containing Nutrient Mixture F-10 (3 mL), increasing concentrations of glucose (1, 2, 5, 10, or 15 mM), and 1 microCi of [U-14C]glucose. Luteal tissue collected at the middle stage of the estrous cycle had greater (P less than .04) rates of glucose uptake (17.8 vs 12.1 ng/mg of wet tissue per min) and oxidation (138.6 vs 67.7 pg/mg of wet tissue per min) and a lower (P less than .02) rate of metabolism of glucose to lactate (10.5 vs 13.3 ng/mg of wet tissue per min) than tissue collected at the late stage. Compared to tissue collected at early and late stages, endometrial tissue collected at the middle stage of the estrous cycle had a similar (P greater than .5) rate of glucose uptake (29.6 vs 27.8 and 26.1 ng/mg of wet tissue per min), a lower (P less than .02) rate of metabolism of glucose to lactate (13.8 vs 16.6 and 16.4 ng/mg of wet tissue per min), and a greater (P less than .1) rate of oxidation of glucose (84.1 vs 65.8 and 72.3 pg/mg of wet tissue per min). In Exp. 2, the previously gravid uterine horn was collected at surgery on d 20 (n = 8) or 30 (n = 7) after calving, or the uterine horn ipsilateral to the preovulatory follicle was collected at first observed estrus after calving (n = 8). Uterine tissue obtained at first estrus after calving had a greater (P less than .003) rate of glucose uptake and oxidation (88.8 vs 77.3 and 75.5 pg/mg of tissue per min) than d-20 or d-30 endometrium.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗

PubMed1992

Effects of gossypol and cottonseed products on reproduction of mammals.

Gossypol is a toxic factor indigenous to the cotton plant genus Gossypium. Concentrations of free gossypol contained in feedstuffs such as whole cottonseed and cottonseed meals vary considerably. Nonruminant animals are particularly sensitive to the toxic effects of gossypol, whereas ruminants are somewhat more resistant. Signs of gossypol toxicosis in nonruminants, preruminants, and ruminants are similar and include labored breathing, dyspnea, decreased growth rate, and anorexia but are not pathognomonic. Postmortem findings include generalized edema and congestion of lungs and liver, fluid-filled thoracic and peritoneal cavities, and degeneration of heart fibers. The antifertility effect observed in many nonruminant species is overshadowed by toxic effects, particularly in females. Gossypol seems to disrupt estrous cycles, pregnancy, and early embryo development in females of all nonruminant species studied. Probable mechanisms include an endocrine effect on the ovary as well as a cytotoxic effect on the uterus or embryo. The female ruminant seems to be relatively insensitive to the antifertility effect of gossypol; however, in vitro data indicate some inhibition of embryonic development and ovarian steroidogenesis. The antifertility effect of gossypol has been studied most in males of nonruminant species. The effects of gossypol in the male are both dose- and time-dependent. At effective doses, gossypol causes males to be infertile because of sperm immotility and depressed sperm counts. Specific mitochondrial damage in the tails of spermatozoa seems to render them immotile, and extensive damage to germinal epithelium may be responsible for depressions in spermatogenesis. In ruminant males fed diets containing gossypol, ejaculated sperm appears normal under light microscopy. The integrity of the membrane of sperm cells may be damaged. Extensive damage to the germinal epithelium has been shown in both rams and bulls fed diets containing gossypol and is of major concern.

Animals↗

PubMed1991

Oxytocin-induced prostaglandin release from perifused bovine caruncular and intercaruncular endometrial tissue on days 20, 30 and at first estrus postpartum.

Twenty-two multiparous Brahman x Hereford F1 cows were utilized to determine the effect of oxytocin (OT) on prostaglandin F2 alpha (PGF) release from caruncular and intercaruncular endometrial tissues and prostaglandin E2 (PGE) release from intercaruncular tissue. The previously gravid uterine horn was removed on d 20 postpartum (n = 7), on d 30 postpartum (n = 7) or the uterine horn ipsilateral to the dominant follicle was removed 12-18 h after onset of first behavioral estrus postpartum (ES; n = 8). Tissues (200 mg wet wt) were cultured in Nutrient Mixture F-10 medium in a perifusion system. The medium and tissues were aerated with 95% O2: 5% CO2 and temperatures were maintained at 39 degrees C. The flow rate was 100 microliters/min and fractions were collected at 20 min intervals for 400 min. After a 2 h settling phase, the tissues were challenged with 1, 2 or 4 micrograms [Asu1,6]-OT/ml of media for 1 h. Basal release of PGE and PGF on d 20 was greater than on d 30 and at ES (P less than .02) which were similar. All doses of OT increased PGE and PGF with both remaining elevated throughout the duration of the perifusion (P less than .008). However, there were no differences among doses. Release of PGE in response to OT on d 20 and 30, was higher than at ES (P less than .008). More PGF was released in response to OT from intercaruncular than caruncular tissue on d 20 (P less than .0001) and at ES (P less than .003). Release of PGF in response to OT on d 20 was higher (P less than .0001) than on d 30 and d 30 was higher than at ES (P less than .007). Basal and OT-induced release of PGE and PGF declined as day postpartum increased. We conclude that intercaruncular tissue released more PGF than caruncular tissue and both intercaruncular and caruncular tissue responded to OT with a sustained release of prostaglandins in a non-dose-dependent manner on d 20, 30 and at ES postpartum.

Animals↗

PubMed1990

Bovine cells expressing bovine herpesvirus 1 (BHV-1) glycoprotein IV resist infection by BHV-1, herpes simplex virus, and pseudorabies virus.

We expressed the bovine herpesvirus 1 (BHV-1) glycoprotein IV (gIV) in bovine cells. The protein expressed was identical in molecular mass and antigenic reactivity to the native gIV protein but was localized in the cytoplasm. Expressing cells were partially resistant to BHV-1, herpes simplex virus, and pseudorabies virus, as shown by a 10- to 1,000-fold-lower number of plaques forming on these cells than on control cells. The level of resistance depended on the level of gIV expression and the type and amount of challenge virus. These data are consistent with previous reports by others that cellular expression of the BHV-1 gIV homologs, herpes simplex virus glycoprotein D, and pseudorabies virus glycoprotein gp50 provide partial resistance against infection with these viruses. We have extended these findings by showing that once BHV-1 enters gIV-expressing cells, it replicates and spreads normally, as shown by the normal size of BHV-1 plaques and the delayed but vigorous synthesis of viral proteins. Our data are consistent with the binding of BHV-1 gIV to a cellular receptor required for initial penetration by all three herpesviruses and interference with the function of that receptor molecule.

Animals↗

PubMed1990

Oxytocin-induced changes in plasma 13,14 dihydro-15-keto prostaglandin F2 alpha concentrations on days 10, 20 and 30 postpartum in the bovine.

Eighteen suckled Brahman cows were allotted randomly to treatments arranged in a three-period crossover design according to calving date and prior treatment such that each cow received 30, 150 and 300 IU oxytocin (OT) i.v. on d 10, 20 or 30 postpartum. Blood was collected via an indwelling jugular catheter every 15 min for 195 min. Samples collected before OT administration were used to determine basal plasma 13,14-dihydro-15-keto prostaglandin F2 alpha (PGFM) concentration. Day, time and the day X dose interaction affected PGFM (P less than .0001). All doses of OT elevated PGFM on all days postpartum (P less than .0001). Basal PGFM was greater (P less than .0001) on d 10 (252.2 +/- 51.2 pg/ml) than on d 20 (78.2 +/- 14.8 pg/ml) or on d 30 (64.8 +/- 7.4 pg/ml). The rise in PGFM in response to OT was greatest on d 10 and decreased (P less than .001) with increasing days postpartum. On d 10, 150 IU of OT caused a greater (P less than .0007) rise in PGFM than either 30 or 300 IU. On d 20, the 300-IU dose raised PGFM more (P less than .005) than either 30 or 150 IU, whereas on d 30 no differences among doses were detected. Cows had higher basal PGFM and a greater response to OT on d 10 postpartum than on d 20 or 30; cows were more responsive on d 20 than on d 30. All doses of OT elevated PGFM at all three times postpartum; however, differences between doses were not detected.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗

PubMed1989

The effect of bovine herpesvirus type 1 glycoproteins gI and gIII on herpesvirus infections.

We expressed the bovine herpesvirus type 1 (BHV-1) glycoproteins, gI and gIII, in bovine cells using a bovine papillomavirus vector. The proteins expressed by these cells had the same Mr as the native BHV-1 proteins and monoclonal antibodies detected no differences in their antigenic structure. Cells expressing gI were infected with either BHV-1 or herpes simplex virus type 1 (HSV-1). The number of plaques in gI-expressing cells was similar to that seen with normal fibroblasts infected with BHV-1 or HSV-1. However, BHV-1 or HSV-1 plaques produced in gI-expressing cells were smaller and darker than those seen in normal fibroblasts indicating an interference with cell-to-cell transmission or cellular lysis. Virus growth curves and [35S]methionine labelling of BHV-1-infected gI-expressing cells showed no difference in virus production, virus protein synthesis or cellular protein shutdown when compared to BHV-1-infected normal cells. This led us to conclude that the gI protein may interfere with a cellular protein(s) responsible for the cytopathic effects of BHV-1 infection. Cells expressing gIII were fully susceptible to BHV-1 infection.

Animals↗

PubMed1987

Efficacy of actaplanin fed on a twice-weekly basis to grazing stocker heifers.

Brahman (16), Brangus (32) and Simmental X Brahman (16) open heifers were assigned to four treatment levels and two body weight categories. The weight categories were light (250 kg) and heavy (306 kg). Treatment groups were 0, 600, 900 and 1,200 mg actaplanin.head-1.feeding-1 with feeding only on Monday and Friday. Heifers were group-fed .91 kg/head of supplement (95% soybean meal: 5% molasses) containing actaplanin. Heifers grazed eight Coastal bermudagrass pastures throughout the experiment (July 29 to November 15, 1982). After 75 d on experiment, an additional 1.36 kg of a commercial 14% crude protein supplement was fed on the days that actaplanin was not fed. All heifers were weighted and rotated among pastures at 14-d intervals (last weight period was 11 d) as an attempt to equalize forage availability among groups. Over the entire experiment (109 d), light-weight heifers that were fed actaplanin responded (P less than .01) with greater average daily gain (ADG; .37, .35, .21 kg/d; 600, 900, 1,200) compared with controls (0 mg; .18 kg/d). A trend in response (P less than .10) to actaplanin feeding occurred for heavy-weight heifers (.23, .38, .34 kg/d; 600, 900, 1,200 mg vs controls (.22 kg/d). For light-weight heifers, ADG decreased lineraly (P less than .01) as actaplanin increased from 600 to 1,200 mg, while ADG for heavy-weight heifers increased linearly (P less than .10).(ABSTRACT TRUNCATED AT 250 WORDS)

Administration, Oral↗

PubMed1984

Experimental paraquat poisoning--histological, electron microscopic and autoradiographic changes in the lung.

Paraquat is a potent and widely used herbicide which acts as a specific pulmonary toxin and causes lung fibrosis in man and animals. Some controversy still exists concerning the details of the morphogenesis of the pulmonary lesions. The lungs of rats exposed to intravenous injections of paraquat and sacrificed 6-24 days later were examined by light and electron microscopy. Autoradiography was used to detect possible paraquat accumulation in the lung 5 hours after a single intravenous injection. The findings on microscopy suggested an acute phase of damage to alveolar lining epithelium followed by epithelial regeneration. The most pronounced light and electron microscopic findings were: signs of disruption of the alveolar wall; type II alveolar epithelial hyperplasia; mobilization of mononuclear cells, and migration and accumulation of fibroblast-like cells in the intra-alveolar and interstitial spaces. After three equally spaced intravenous injections of paraquat signs of interstitial connective tissue proliferation could be seen. Autoradiography showed low-grade radioactivity over the alveolar wall, indicating possible active uptake of paraquat by alveolar epithelium; this coincides with in vitro evidence of an active transport mechanism for paraquat by alveolar epithelial cells.

Animals↗

PubMed1984

The effect of paraquat on the in vitro activity of cytosol, mitochondrial and microsomal enzyme systems.

Subcellular fractions (mitochondria, microsomes and cytosol) were prepared from the lungs of rabbits and rats to investigate the effects of paraquat (Aldrich Laboratories) on the activity of some cytosol and mitochondrial dehydrogenases and on the microsomal respiration and reduced pyridine nucleotide oxidation rate. The normal basal oxygen consumption of rabbit lung microsomes was 1,9 +/- 0,3 nmol O2/mg microsomal protein/min, and the oxidation rates of reduced nicotinamide-adenine dinucleotide phosphate (NADPH) and reduced nicotinamide-adenine dinucleotide (NADH) were 4,29 +/- 0,53 and 4,0 +/- 0,55 nmol/mg microsomal protein/min respectively. One molecule of oxygen can therefore oxidize two molecules of NADPH or NADH, and the generated hydrogen peroxide is probably immediately broken down by the catalase activity of the normal lung microsomal preparation. When Aldrich paraquat (1,0 mM) was added to microsomes metabolizing NADPH (0,5-0,75 mM), both the rate of oxygen consumption and the generation of nicotinamide-adenine dinucleotide phosphate (NADP) were significantly (P less than 0,001) stimulated over the first 5 minutes, and thereafter returned to within basal limits. When microsomes were preincubated with 1,0 mM paraquat before NADPH was added, the oxygen consumption was substantially lower (10,01 +/- 1,01 nmol oxygen/mg microsomal protein/min), while the NADPH oxidation rate was almost similar to the basal rate in the absence of paraquat. This resulted in a striking dissociation in the H/O ratio under these circumstances. The addition of potassium cyanide (KCN) (5.0 mM) prior to paraquat pre-incubation and followed by the addition of NADPH restored the stimulatory effect of paraquat on microsomal respiration and on NADPH oxidation rate.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗

PubMed1981

[The effect of steroid therapy on cytological and histopathological changes during experimental extrinsic allergic alveolitis (hypersensitivity pneumonitis)].

Acute extrinsic allergic alveolitis was experimentally induced in rabbits using horseradish peroxidase (HRP) as antigen. Broncho-alveolar lavage was performed on the excised lungs and total and differential cellular yields determined, and correlated with the histopathological changes in the lungs as well as the total and differential white blood cell counts. After a single parenteral immunization with HRP without adjuvants, and weekly aerosol challenges with nebulized HRP solution for 3 consecutive weeks, a 3-fold increase in the total cell count as well as a very pronounced rise in the percentage of lymphocytes was noticed. Histopathologically, the bronchi-associated lymphoid tissue (BALT) became more prominent, an increase in the number of foreign body giant cells was noticed and a focal interstitial and intra-alveolar accumulation of lymphocytes, granulocytes and macrophages could be demonstrated, as well as a mild hyperplasia of type 2 alveolar epithelial cells. Intramuscular injections of methylprednisolone acetate (Depo-Medrol) every 72 hours induced a pronounced peripheral lymphopenia, thymic involution and an almost complete disappearance of the BALT in both the control and HRP-challenged rabbits. Similarly, a marked decrease in the total cell count and percentage of lymphocytes was noticed in the broncho-alveolar fluid of the animals with hypersensitivity pneumonitis. No signs of interstitial or intra-alveolar reactions were seen in the lungs of the experimental animals after 3 weeks of aerosol antigen challenge when treated with steroids. Collectively, these data suggest that the development of hypersensitivity pneumonitis was, at least in part, due to a cell-mediated immunological reaction in the lung. This animal model in which steroid suppression of experimental allergic alveolitis has been demonstrated, may be employed to elucidate the cellular pathogenesis of this disease process.

Alveolitis, Extrinsic Allergic↗

PubMed1980

Surface binding and uptake of polycationic ferritin by neonatal piglet intestinal epithelium.

Pieces of small intestine from newborn, I- and 6-day-old piglets were incubated in vitro and ligated segments were incubated in vivo with polycationic ferritin (PCF) to determine the distribution and possible role in protein uptake of anionic sites on membranes at the luminal surfaces of the epithelial cells. Most PCF binding to the absorptive cells occurred on the upper third or half of the villi and to some non-absorptive cells (tuft cells) throughout the length of the villi. Pieces of intestine which were fixed before incubation had PCF on microvilli and apical invaginations of absorptive cells, but none in the sub-apical tubules. When samples were incubated with PCF in vitro before fixation PCF was bound to the surfaces of microvilli of absorptive cells and to the membranes lining the apical invaginations, some sub-apical vesicles and some sub-apical tubules in all age-groups. In vivo experiments with longer incubation times resulted in a similar distribution, with increased amounts of PCF in the sub-apical tubules in samples from newborn and 1-day-old piglets only. In the newborn there were small vesicles containing PCF which had apparently moved further into the cells. At the same concentration (2 mg/ml) ferritin did not enter the sub-apical tubules; but it did enter and was taken up by the cells in the presence of 4% (w/v) serum albumin. It is concluded that the mechanism of protein uptake does not involve the microvillar membrane and that non-specific interaction with the invaginated plasma membrane, as a function of charge-density, leads to opening of the sub-apical tubular system to protein. Movement further into the cell depends on other factors.

Animals↗

PubMed1978

The adrenal cortex in hypercholesterolaemic rabbits: histochemical and electron microscopical changes.

The adrenals of rabbits on a cholesterol-rich diet for 35 days show histopathological changes, a marked increase in weight and a lowering in the ascorbate content. A focal increase in the neutral lipid and cholesterol content was noted mostly in the inner cortical zones; and a characteristic acid phosphatase-positive pattern in areas of infiltrating cells, and an alkaline phosphatase-positive reaction in heterophils in the infiltrated areas. Electron microscopy confirmed that the zona glomerulosa cells were relatively normal in hypercholesterolaemic rabbits, while necrosis and fibrosis were very obvious in the inner two zones. The cellular infiltrate was shown to consist of large, granular mononuclear cells, heterophils, eosinophils, stromal phagocytes, lymphocytes and plasma cells. The possibility that the reaction was of an immunological nature is considered. The morphology of the adrenals of rabbits which were on a cholesterol-rich diet for 35 days and on a normal diet for 6 weeks afterwards, was indistinguishable from that of those rabbits killed after 35 days on a cholesterol-rich diet.

Acid Phosphatase↗

PubMed1978

Ultrastructure of the capsule of the rabbit adrenal gland.

The capsule of the normal rabbit adrenal gland was examined with the electron microscope, and three layers are described. The outermost layer consists of collagen and elastic fibrillae, with cytoplasmic processes of fibroblasts in between. Myofibroblasts with parallel cytoplasmic filaments, dense bodies at the inner surface of the plasma membrane and an irregular nuclear outline were seen in the middle layer. The presence of unmyelinated nerves and an axon terminal near to the myofibroblasts of the middle layer raised the possibility of innervation of these cells which could have a contractile function. The basal lamina of the fenestrated capilaries in the inner vascular layer is occasionally fused with that of the outer zona glomerulosa, suggesting a probable route for blood supply and secretion.

Adrenal Glands↗

PubMed1976

The histochemical localization of cholesterol in formalin-fixed and fresh frozen sections.

The premixed cholesterol reagent (acetic acid, sulphuric acid, ferric chloride and phosphoric acid) employed in a spectrophotometric method for serum cholesterol determinations was applied to frozen sections of various rat and rabbit tissues. The histochemical reaction was compared with the results obtained by the classical Schultz reaction. The same bluish-green color was observed after treatment of formalin-fixed and fresh cryostat sections incubated in 2.5% iron alum at 37 C for 3 days. The premixed cholesterol reagent is stable for years at room temperature if stored in a brown bottle and is thus readily available for routine use.

Adrenal Glands↗