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C C RANDALL

Publications and source records attributed to C C RANDALL.

At least 19 recordsLinked to original sources
PubMed1965

FINE STRUCTURE OF THE COAT AND NUCLEOID MATERIAL OF FOWLPOX VIRUS.

Hyde, James M. (University of Mississippi School of Medicine, Jackson), Lanelle G. Gafford, and Charles C. Randall. Fine structure of the coat and nucleoid material of fowlpox virus. J. Bacteriol. 89:1557-1569. 1965.-Several morphological forms characteristic of the poxvirus group were demonstrated for fowlpox virus with neutral phosphotungstic acid (PTA). Viral particles (purified from viral inclusion bodies) stained with uranyl acetate (UA) and shadowed with platinum were shown to have an external knobby surface not evident with PTA. The external coat of freshly purified viral particles seemed intact, but as the preparation aged, it appeared to unwind, resulting in twisted "rope-like" structures. This process was facilitated by use of 1% trypsin, and three dense fibrils were identified with UA within the partially detached viral coat. Studies with alkaline PTA (pH 9) were interpreted as revealing a complex nucleoid, but solutions above this pH damaged the particles. The morphology of the nucleoid was better depicted in ultrathin sections of whole virus which, when stained with UA, revealed dense coiled threads. Treatment of virus with sodium lauryl sulfate exposed an underlying coat consisting of small subunits approximately 40 A in diameter. Of great interest was the demonstration that the detergent removed strands of deoxyribonucleic acid (DNA) from the virus without destroying the contour of the particle. The origin of the strands was definitely the fine uranophilic, coiled threads of the nucleoid, which probably represent the DNA molecule(s). That the extracted material was largely DNA was proved by digestion with deoxyribonuclease and resistance to ribonuclease and trypsin. These studies illustrate how a variety of electron microscopic techniques may be utilized alone or in combination to reveal hitherto undescribed fine structure of viral particles.

Animals↗
PubMed1964

COMPOSITION OF FOWLPOX VIRUS AND INCLUSION MATRIX.

Randall, Charles C. (University of Mississippi School of Medicine, Jackson), Lanelle G. Gafford, Robert W. Darlington, and James M. Hyde. Composition of fowlpox virus and inclusion matrix. J. Bacteriol. 87:939-944. 1964.-Inclusion bodies of fowlpox virus infection are especially favorable starting material for the isolation of virus and inclusion matrix. Electron micrographs of viral particles and matrix indicated a high degree of purification. Density-gradient centrifugation of virus in cesium chloride and potassium tartrate was unsatisfactory because of inactivation, and clumping or disintegration. Chemical analyses of virus and matrix revealed significant amounts of lipid, protein, and deoxyribonucleic acid, but no ribonucleic acid or carbohydrate. Approximately 47% of the weight of the virus and 83% of the matrix were extractable in chloroform-methanol. The lipid partitions of the petroleum ether extracts were similar, except that the phospholipid content of the matrix was 2.2 times that of the virus. Viral particles were sensitive to diethyl ether and chloroform.

Animals↗
PubMed1964

REPLICATION OF A DEOXYRIBONUCLEIC ACID VIRUS IN THYMINE-DEFICIENT MAMMALIAN CELLS.

Gentry, Glenn A. (University of Mississippi School of Medicine, Jackson), Lucy A. Lawson, and Charles C. Randall. Replication of a deoxyribonucleic acid virus in thymine-deficient mammalian cells. J. Bacteriol. 88:1324-1328. 1964.-Equine abortion virus (EAV), a deoxyribonucleic acid (DNA) virus, causes the degradation of host cell DNA to acid-soluble components in the L-M cell. It was hypothesized that inhibitors of DNA synthesis such as 5-fluorodeoxyuridine (FUdR) and amethopterin, which act by causing a deficiency in thymidine (TdR) nucleotides, would not block virus replication because the breakdown of the host cell DNA would supply sufficient TdR nucleotides for synthesis of viral DNA. The drugs were added 24 hr prior to the virus, amethopterin at 10(-6)m with adenosine included as a purine source, or FUdR at 10(-5)m. In either case, control experiments indicated that the thymineless state was achieved in the host cells. When EAV was added, neither FUdR nor amethopterin had any effect on the production of infectious virus, as determined by plaque assay, thus supporting the hypothesis.

Animals↗
PubMed1963

DEGRADATION OF DEOXYRIBONUCLEIC ACID AND ALTERATION NUCLEIC ACID METABOLISM IN SUSPENSION CULTURES OF L-M CELLS INFECTED WITH EQUINE ABORTION VIRUS.

Randall, Charles C. (University of Mississippi School of Medicine, Jackson) and Barbara M. Walker. Degradation of deoxyribonucleic acid and alteration of nucleic acid metabolism in suspension cultures of L-M cells infected with equine abortion virus. J. Bacteriol. 86:138-146. 1963.-Metabolic alterations in log-phase suspension cultures infected with equine abortion virus (EAV) were determined in L-M cells simultaneously labeled or prelabeled with H(3)- or C(14)-thymidine. Although infection produced an early stimulation of the uptake of labeled thymidine (TdR) into the acid-soluble fraction of concurrently labeled cells, incorporation of the isotope into deoxyribonucleic acid (DNA) was progressively inhibited. The specific activity of infected-cell DNA was 48% of the control at 24 hr. The rate of incorporation of isotope from 12 to 24 hr was 43 and 13 counts per min per mug of DNA per hr for control and infected cultures, respectively. Owing to degradation of DNA, synthesis could not be accurately determined with the concurrently labeled cells. On the other hand, with prelabeled cells, quantitative isotopic methods could be used to determine the amount of DNA synthesized by measuring dilution of specific activity, even though infection triggered degradation of DNA into acid-soluble components. With this method, the DNA synthesized in infected cultures for 24 hr was approximately five times greater than the slight net increase determined by the diphenylamine reaction. The specific activity of infected-cell DNA decreased and then remained fixed after 24 hr, with 53% of the radioactivity appearing in the medium by 48 hr. No radioactive CO(2) was detected as a consequence of DNA degradation. Infected cells lost ribonucleic acid (RNA) as well as DNA; RNA and DNA were reduced by 64 and 50%, respectively, at 48 hr. The degradation of DNA was effectively inhibited by chelating agents in situ and is thought to be due to a deoxyribonuclease. Preliminary experiments with extracts of infected cells support this observation. The relationship of deoxyribonuclease to the synthesis of viral DNA remains to be determined.

Animals↗
PubMed1962

Bases of the nucleic acid of fowlpox virus and host deoxyribonucleic acid.

Randall, Charles C. (University of Mississippi School of Medicine, Jackson), Lanelle G. Gafford, and Robert W. Darlington. Bases of the nucleic acid of fowlpox virus and host deoxyribonucleic acid. J. Bacteriol. 83:1037-1041. 1962.-The nucleic acid of fowlpox virus obtained from purified inclusions was identified as deoxyribonucleic acid (DNA). The base ratios determined on whole virus and on isolated DNA were practically identical. The molar ratios of purines to pyrimidines were approximately unity. The ratio of adenine plus thymine to guanine plus cytosine was 1.8. The base ratios of DNA obtained from chick red cells were approximately the same as other animal DNA preparations reported in the literature. It is of interest that the molar proportions of host DNA are significantly different from viral DNA.

Adenine↗