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Biomedical subjects

C C Romano

Publications and source records attributed to C C Romano.

9 recordsLinked to original sources
PubMed2001

Imbalance of IL-2, IFN-gamma and IL-10 secretion in the immunosuppression associated with human paracoccidioidomycosis.

Patients with paracoccidioidomycosis (PCM) display a certain degree of immunecompromise characterized by lymphocyte hyporesponsiveness to the main Paracoccidioides brasiliensis antigen (gp43). To determine whether cytokines are involved in this state, we evaluated the secretion of IL-2, IL-10 and IFN-gamma by peripheral blood mononuclear cells (PBMC) from patients with the acute (AF) and chronic (CF) forms of PCM and from healthy, P. brasiliensis-sensitized subjects. gp43-stimulated PBMC from healthy subjects produced substantial amounts of IL-2, IFN-gamma and IL-10, whereas PBMC from AF and CF patients produced low levels of IL-2 and IFN-gamma but substantial amounts of IL-10. Phytohaemagglutinin-induced cytokine secretion was comparable among AF and CF patients and healthy subjects, suggesting integrity of non-specific cellular immune mechanisms in PCM. gp43-pulsed adherent cells, but not non-adherent cells, were the main source of IL-10. Moreover, IL-2 and IFN-gamma secretion correlated inversely with the amount of specific antibodies produced by patients and healthy subjects. Our results suggest that the imbalance in cytokine production of patients with PCM plays a role in the gp43-hyporesponsiveness and the marked (non-protective) antibody production of these patients.

Adolescent↗
PubMed2001

Characterization of the cellular immune function of patients with chronic mucocutaneous candidiasis.

Chronic mucocutaneous candidiasis (CMC) is a rare syndrome characterized by persistent and refractory infections of the skin, nails and mucosal tissues by yeasts of the genus Candida. Defects in the cellular limb of the immune system are well documented in CMC patients, but non-specific immune defects, such as myeloperoxidase deficiency or phagocyte chemotaxis disorders, have also been described. Nonetheless, the underlying defect(s) remains poorly understood, and further studies are required. We studied eight CMC patients without endocrinopathies, who showed (i) low normal proliferative response to phytohaemagglutinin (PHA), (ii) partially defective response to pokeweed mitogen (PWM), and (iii) impaired response to Candida and PPD antigens. Furthermore, peripheral blood mononuclear cells (PBMC) from CMC patients produced lower levels of type-1 cytokines (IL-2 and interferon-gamma) in response to Candida antigens, compared with control individuals. Conversely, we did not observe an enhancement of IL-4 and IL-10 in the patients, suggesting that, even though Th1 cytokines are decreased, the Th2 response is not increased in CMC. Nevertheless, the synthesis of these cytokines was normal when induced by PHA. We also observed an increased antigen-induced apoptosis in lymphocytes from the patients compared with controls, and this applied both to Candida and PPD antigens. Lastly, innate immunity defects were investigated. We observed an impairment of natural killer activity against K-562 target cells in half of the studied patients. These findings corroborate the extensive clinical and laboratory variability of CMC, which requires further studies on a larger number of patients to be better understood.

Adolescent↗
PubMed1997

Diagnostic equivalence of conventional and fast spin echo magnetic resonance imaging of the anterior cruciate ligament of the knee.

Many techniques and pulse sequences have been devised for the assessment of the anterior cruclate ligament. The present study compares fast spin echo (FSE) imaging to conventional spin echo imaging at a field strength of 1.5 T in an effort to determine if these sequences are diagnostically equivalent. Where available, arthroscopy was also done. A total of 52 patients were imaged using both FSE and conventional spin echo sequences. Eight volunteers were used as controls. Arthroscopy was performed on 10 patients. The anterior cruciate ligament was assessed in a blinded fashion by three radiologists. The Kappa statistic was then used to determine the percentage agreement between FSE and conventional spin echo imaging. Fast spin echo sequencing demonstrated a sensitivity of 100%, a specificity of 94.8% and an accuracy of 96.3% when compared to arthroscopy. Conventional spin echo imaging and arthroscopy had a sensitivity of 100%, specificity of 84.6% and an accuracy of 88.9%. The remaining 34 patients who did not undergo arthroscopy were followed clinically because clinical and imaging findings were not suggestive of ACL tears. These demonstrated 72% agreement between FSE and conventional spin echo imaging using the Kappa statistic, with regards to calling ACL normal or having only a low-grade partial tear. Fast spin echo imaging produces images of the anterior cruciate ligament that have similar diagnostic accuracy to conventional spin echo images (P < 0.05) within a much shorter scan time. These results however, require further validation in a larger group, preferably with arthroscopic correlation.

Adolescent↗
PubMed1994

Adamantinoma of the tibia: plain-film, computed tomography and magnetic resonance imaging appearance.

The authors describe the computed tomography (CT) and magnetic resonance imaging (MRI) characteristics of adamantinoma of the tibia in a 29-year-old man. Adamantinoma is a rare, aggressive osteolytic lesion occurring primarily in the diaphyseal portion of long bones. Because of its rarity the MRI features have been infrequently reported. In this case MRI provided more accurate information about the extent and invasiveness of the tumour than either plain radiography or CT.

Adult↗
PubMed1994

Imaging after arthroplasty.

Arthroplasty is now an important and frequently performed orthopedic procedure. This article focuses on the imaging of abnormalities frequently encountered in patients who have undergone arthroplasty. The emphasis is on plain film analysis, although arthrography, computed tomography and nuclear medicine are also discussed. Among the problems illustrated are loosening of the prosthesis, component fatigue or failure, and infection. The authors recommend techniques to optimize imaging. Although most of the entities illustrated involve hip arthroplasty, the principles are valid for arthroplasty at other sites.

Arthrography↗
PubMed1993

Magnetic resonance imaging of a giant nonfunctioning retroperitoneal paraganglioma.

The authors describe the magnetic resonance imaging (MRI), computed tomographic, ultrasonographic and angiographic appearance of a giant nonfunctioning retroperitoneal paraganglioma. To their knowledge, the MRI appearance of a nonfunctioning paraganglioma has not been previously described. The MRI characteristics of the nonfunctioning tumour were similar to those of a functioning paraganglioma and included hyperintensity in T2-weighted images and intense enhancement after intravenous administration of contrast agent.

Adolescent↗