[Mononucleosis-like syndrome from adenovirus with typical bacterial analytical characteristics].
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Biomedical subjects
Publications and source records attributed to C Calvo.
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The experience of two units (Gastroenterology and Urology) of Clínica La Luz, Madrid, in the treatment of gallbladder and renal lithiasis using the Dornier MPL-9000 lithotripter is reported herein. This lithotriptor has been utilized in the treatment of all cases of gallbladder calculi for which it is indicated and renal calculi localized high up (kidney), and in those cases that are difficult to treat with other lithotriptors such as patients with kyphoscoliosis, radiolucent calculi, elderly patients, children, etc.). During the first year the gallstone lithotripsy unit was utilized, 401 patients were referred for treatment. Of these, 180 met the criteria for patient selection. (Over 360 have been treated to date.) Sixty of these 180 patients had a single gallstone of up to 2.5 cm. These 60 patients were selected for the study since they comprised a group where the best results could be expected. The aim of studying this patient group was to evaluate the results achieved by lithotripsy and the short and medium-term outcome in this ideal group of patients. Concerning renal lithiasis, these are the first 100 cases treated with the MPL-9000 (currently over 420 have been treated). Eighty cases had a single calculus (60 caliceal and 20 pyelic), 15 had multiple calculi, and 5 had a staghorn stone. Treatment for gallbladder and renal lithiasis was exclusively by shock waves; i.e., as monotherapy with no other auxiliary procedure. The average number of shock waves used was 2,350 with a mean kV of 18 for gallbladder lithiasis.(ABSTRACT TRUNCATED AT 250 WORDS)
The outcome of 60 cases of single biliary stone with a maximum diameter of 25 mm was evaluated. The patients were symptomatic, with functioning gallbladder, and were selected from a group of 180 to evaluate the effects of electrohydraulically generated shockwaves on a priori ideal cases for this procedure. In seven patients fragmentation was not achieved, probably because of the pigmentary character of the stone, that was later verified at cholecystectomy. In 20 out of the 53 in whom fragmentation was satisfactory (38%), fragments were not observed after 24 hours. 32 patients (54%) had a stone-free gallbladder 3 months afterwards; at six months, the number had increased to 40 (66%). This rate becomes 77% if only the cases with fragmentation are considered. In 29% of patients episodes of colicky biliary pain developed as complication. No case of pancreatitis or stuck in gallstone were observed. We conclude that shock waves appear as an excellent therapeutic alternative in single gallstones up to 25 mm in size.
The concentrations of polychlorinated biphenyls have been determined over a period of 1 year in surface sediments sampled monthly and semi-monthly in the lagoon of Venice. At the sampling station, due to the poor water circulation and an over-abundance of nutrients, macroalgae thrive in spring-summer and standing crops greater than 12 kg m-2 were determined. As a consequence of rapid algal decomposition, sediment PCB concentrations were observed to increase. In particular, on occasions when anoxia occurred at the end of July and all the biomass had decayed, PCB concentrations increased by more than one order of magnitude. In March, when macroalgae started to grow, concentrations were 13 ng g-1 (dry wt); in July-August they exceeded 100 ng g-1. The sediment PCB concentrations were restored to the previous values after algal biomass became negligible, in November. Concentrations of PCBs in young algae fronds were 27 +/- 12 ng g-1, and 121 +/- 4 ng g-1 in older fronds.
Yersinia strains either urease-positive or urease-negative were examined for precipitation of struvite in human urine at 25 and 37 degrees C. All urease-positive strains and 8 out of 10 urease-negative strains showed the ability to produce these crystals. Incubation time required for precipitation was longer for urease-negative strains and quantity of struvite formed was higher in urease-positive ones. Regarding incubation temperature, no significant influence has been observed.
We report a collective tuna fish poisoning which developed on June 10, 1988, in a staff dining room in Castellón. Twenty-one people were involved (attack rate 42.9%). The major symptoms were diarrhea, abdominal pain, headache, facial flushing and oral burning. The mean duration of the symptoms was 15 hours. The ingestion of tuna fish was significantly associated with the illness (p less than 0.001) when the other foodstuffs were controlled. Tuna fish had been defrosted at room air temperature during 14 hours. The analysis of several foodstuffs (there were no tuna fish remains available) and the food handling staff did not disclose pathogens. We discuss the etiology, pathogenesis, epidemiology and control of this type of food poisoning.
Extracellular formation of struvite crystals by Yersinia enterocolitica, Y. frederiksenii, Y. kristensenii and Y intermedia strains was investigated. Precipitation of crystalline structures was found with 19 of the 187 strains tested, its formation being more frequently observed at 25 degrees C than at 37 degrees C. Production of struvite was greater in Y. enterocolitica strains belonging to biotype 1, serogroup 0:7,8 and lysotype Xz, than observed in other phenotypes. Quantitative assay in a liquid medium showed that struvite formation began after 3 d of incubation; production of these crystals increased up to 15 d. Crystalline structures were examined using electron microscopy and their extracellular formation was observed.
134 patients with mild to moderate hypertension received nitrendipine during 6 months in an increasing fashion. Therapeutic goal was achieved in 92% of the patients (DBP less than 95 mmHg) in which BP decreased from 168.4 +/- 13.7/105.0 +/- 1.1 to 140.9 +/- 12.7/84.4 +/- 6.6 (p less than 0.001). In patients whose BP was not controlled with a single initial doses of 20 mg/day, the degree of control was similar with 40 mg single dose and 20 mg twice a day. The most frequently encountered undesirable effects were those caused by the vasodilator action of the drug. No changes in blood glucose and plasma lipid levels were found.
In diabetic patients, hyperglycaemia results in the non enzymatic glycation of many proteins including apolipoprotein A-I. We purified glycated apo A-I and compared its lipid binding properties to those of normal apo A-I. Analysis of tryptophan fluorescence spectra and of fluorescence quenching in the presence of iodine showed that glycation of apo A-I induces a decrease in the stability of the lipid-apoprotein interaction and in that of the apoprotein self-association. Repetitive ultracentrifugations of High Density Lipoprotein (HDL) samples containing radioiodinated apo A-I or glycated apo A-I revealed that glycation of the apoprotein facilitates its dissociation from HDL. These results suggest that the non enzymatic glycation of apo A-I may affect the structural cohesion of HDL particles.
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The purpose of this report is to describe a seven-years-old patient with an idiopathic perforation of the common bile duct. This disorder is more frequent in the first weeks of life, and the clinical features are not well known. Properly treated, the majority of infants survive without sequelae. Furthermore, a review of the literature is made.
One strain of Yersinia enterocolitica and one strain of Y. intermedia were grown in peptone water at 25 or 37 degrees C, or in ground water at 15 degrees C. Similar growth rates were observed when these strains were cultivated separately in the same media and at the same temperature. Mixed cultures at 37 degrees C displayed equivalent growth rates. In contrast, mixed cultures incubated at 15 or 25 degrees C were regularly unfavourable to Y. enterocolitica, whereas they did not modify the growth of Y. intermedia. A bacteriophage active on Y. enterocolitica and not on Y. intermedia was characterized from the filtrate of mixed cultures at low temperatures. This phage produced by the lysogenic Y. intermedia strain might be a potential factor responsible for the inhibition of Y. enterocolitica, since no additional antibacterial factor or nutritional competition between Y. intermedia and Y. enterocolitica were found in the mixed cultures.
The production of bacteriocin-like substances by strains of Yersinia frederiksenii, Y. kristensenii and Y. intermedia in broth culture was established. These substances showed a selective activity against Y. enterocolitica, Y. frederiksenii, Y. kristensenii and Y. intermedia strains. Electron micrographs revealed the presence of phage tails in culture media. The production of these substances was detected in cultures grown at 25 degrees C but not in those grown at 37 degrees C, while these bacteriocin-like substances were active at 25 and 37 degrees C. Y. enterocolitica serogroups 0:3 and 0:9 were more susceptible to these bacterin-like substances than strains of Yersinia isolated from environmental sources.
A collective intoxication with chlorine gas in 76 children (21 males, 55 females; the median age was 9.9 years) is reported. They were treated with a combination of oxygen gas (O2) and corticosteroids until the symptoms disappeared.
Two Yersinia enterocolitica strains were able to utilize the products of cephalothin degradation. The utilization of these products was shown by an increase of oxygen uptake by Y. enterocolitica with cephalothin as the only substrate, and by the growth of both strains with the hydrolysis products of cephalothin as sole energy and carbon sources. Nuclear magnetic resonance analysis of the cephalothin degradation reaction demonstrated the progressive disappearance of hydrolysis products. However, the products of benzylpenicillin degradation could not be utilized by Y. enterocolitica.
The incidence of the Reye syndrome in Spain has been retrospectively reviewed from 1980 to 1984. A survey of 57 cases has been available to study. It is pointed out the low frequency (0.12/10(5) 15 years); the low mean age (12 month) due to a higher prevalence in infants; the high mortality rate (49%), proportional to the great number of III and IV Lovejoy's stages; the low correlation with chicken-pox and salycilate-intake in the prodromic period and a better prognosis in patients when barbituric coma was established along with other measures.
The presence of receptors to bacteriophage I of Y. enterocolitica in Y. enterocolitica cells grown at 37 degrees C was investigated. Protein extracts obtained from bacterial cells (strain 4052) grown at 25 degrees or 37 degrees C produced a diminution of free bacteriophage. Cultures of this strain inactivated phage I at 37 degrees C, and high titre suspensions of this phage showed bacteriocin-like activity. According to these results receptor sites must be present in Y. enterocolitica cells incubated at 37 degrees C.