Oligonucleotide-polymer conjugates have been described to improve the sensitivity of an enzyme-linked oligosorbent assay diagnostic test. To understand the influence of their structure and conformation in solution on the efficiency of the test during the capture step, two different ways of synthesizing these conjugates were compared. The first consisted of coupling 5' amino modified oligonucleotides to poly(maleic anhydride-alt-methylvinyl ether) and poly(maleic anhydride-alt-ethylene). The second resulted from direct synthesis of oligonucleotides from poly(maleic anhydride-alt-ethylene) previously grafted onto a controlled pore glass support. The different conjugates were analyzed by size-exclusion chromatography and viscometry. The former method for conjugate synthesis produced aggregates, which was not the case for the latter. These conjugates were then used in the capture phase of a hybridization assay using a HBV DNA target, on a bioMérieux VIDAS instrument. Different parameters were studied, such as the purity of the conjugate solution and the number of oligonucleotides per polymer chain. The amount of conjugate coated on the solid-phase receptacle surface at the time of the capture phase was evaluated by radioactive labeling. Finally, it was demonstrated that conjugates produced an amplification factor of 50 versus the capture oligonucleotide probe used as the reference. The detection limit reached 10(8) copies/mL.

Base Sequence↗

PubMedDate not supplied

The use of (oligonucleotide-maleic anhydride copolymer) conjugates in nucleic acid diagnostic assays: effect of the number of oligonucleotides per polymer on test sensitivity improvement.

(Polymer-oligonucleotide) conjugates were obtained via direct ON synthesis from the poly(ethylene-alt-maleic anhydride: PEMA) grafted onto glass bead surfaces. The effect of the ON number per polymer chain on binding was evaluated through Tm experiments. Results were correlated with ELOSA (Enzyme Linked Oligosorbent Assay) tests run with conjugates in the capture step.

DNA, Viral↗

PubMedDate not supplied

[Foot nerve blocks. (Apropos of 33 cases)].

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Adult↗

PubMedDate not supplied

[Giant cell tumors of the long bones. Curettage-filling].

13 cases of G.C.T. were treated by curetage with only 3 benign and 1 malignant recurrences. The authors are in favour of curetage as a routine treatment provided it is performed at the right time.

Bone Neoplasms↗

PubMedDate not supplied

[Unusual pulmonary metastases of giant cell tumors].

A case of giant cells tumour treated by curetage recurred locally together with pulmonary metastasis. Amputation and lobectomy disclosed no malignancy. In spite of incomplete removal of pulmonary tumours. X rays picture and general status are normal 4 years later. 5 comparable published cases are reported.

Adolescent↗

PubMedDate not supplied