Biomedical subjects

C Chang

Publications and source records attributed to C Chang.

488 records · Page 28Linked to original sources

PubMedDate not supplied

Elimination of malignant tumor cells from human bone marrow using monoclonal antibodies and immunomagnetic beads.

Conditions for depletion of malignant tumor cells from human bone marrow using monoclonal antibodies and immunomagnetic beads were studied. Human hepatoma HA22T/VGH cells labeled with the supravital DNA binding dye Hoechst 33342 were seeded into normal human bone marrow at a ratio of tumor cells to marrow cells of 1:9. The immunobeads used were M-450 magnetic microspheres coated with sheep anti-mouse immunoglobulin. The marrow-tumor cell mixtures were incubated with a panel of antihuman hepatoma cell monoclonal antibodies (9B2, E10A1, and C2E6) at 4 degrees C for 40 min, and then reacted with immunomagnetic beads for 60 min longer. After incubation, the bead-coated tumor cells were removed from the marrow with a magnetic particle concentrator. Tumor residual was measured in a limiting dilution test, and the recovery of marrow progenitor cells was assessed by CFU-GM assay. The efficiency of tumor depletion was closely related to the amount of monoclonal antibody bound to tumor cells and the immunobead/tumor cell ratio. Monoclonal antibodies used in combination were more effective than their individual use alone. Two cycles of purging with both monoclonal antibodies and immunomagnetic beads resulted in an apparent improvement in tumor depletion as compared with one cycle. Using a cocktail of three monoclonal antibodies and purging the marrow for two cycles, 4 to 5 logs of tumor cells could be removed with a sufficient recovery (70%) of CFU-GM. However, multiple purging treatments (> or = 3 cycles) would be apparently damaging to hematopoietic progenitor cells.

Animals↗

PubMedDate not supplied

Acupuncture in the treatment of asthma: a critical review.

BACKGROUND: Increasingly, patients are turning to treatments and drugs that are considered "alternative" or "complementary" as part of their healthcare. In response, the National Institutes of Health (NIH) established an Office of Alternative Medicine (OAM) in 1992 to facilitate identification and evaluation of alternative and complementary therapies. Acupuncture, a therapy that has been used to treat disease in China for approximately 2,500 years has attracted considerable attention. NIH in a consensus statement derived from a Fall of 1997 consensus conference to assess acupuncture has indicated that acupuncture was useful in pain control and maybe a useful adjunct treatment for the management of asthma. Further, the US Food and Drug Administration has removed acupuncture from the "experimental medical devices" category. METHODS: We have extensively searched the literature for reports addressing the use of acupuncture in asthma treatment. We sought these using the National Library of Medicine, the Office of Alternative Medicine's database as well as other databases both English language based as well as other languages that catalog literature pertaining to alternative and complementary therapies. We then reviewed these reports and weighted the validity of the conclusions reached in the reports based on assessment of study design, number of subjects studied, duration of studies, types and number of controls, and statistical analyses used. RESULTS: Data presently in the literature do not provide sufficient support for a useful role for acupuncture in asthma management. CONCLUSIONS: Further properly designed clinical studies examining the use of acupuncture in asthma are extremely important and urgently needed.

Acupuncture Therapy↗