Acquired wisdom in innate immunity.
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Biomedical subjects
Publications and source records attributed to C Colaco.
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A tau peptide, peptide 2R, with capacity for self assembly into filaments was used as a model to test the role of glycation on tau assembly or aggregation. Our results indicate that glycation of that peptide facilitates dimer formation but not assembly into filaments. However, glycation of tau results in the bundling of the tau filaments formed by glycosaminoglycan-induced polymerisation. These results suggest a role of glycation in the formation of covalent links among pre-formed filaments but not in the assembly of those filaments.
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A monoclonal antibody-derived, dry blood grouping plate, based upon the simple disaccharide, trehalose, is described that is indefinitely stable at room temperature and was found to have a 99.8% accuracy when tested against a standard semiautomated assay. This plate can be used by personnel with no specific training to check the recorded A,B,O, and Rhesus blood type of potential transfusion recipients in the field and at the bedside. Trehalose-based reagents may be important for bedside testing and in developing countries where refrigeration is unreliable.