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C Cousin

Publications and source records attributed to C Cousin.

At least 37 records · Page 2Linked to original sources
PubMed1989

Negative effect of a cis-acting pBR322 element on adenovirus E1a gene expression.

A sequence element within plasmid pBR322 has a cis-acting negative effect on the expression of a cloned Ad gene in transient expression assays. The negative element is located between the PvuII and Tth111I restriction sites on pBR322 (nt 2068-2223). This element was also shown to be responsible for the decrease in focus number, when plasmids carrying Ad2 E1 genes were used to transform baby rat kidney cells. In a similar manner, this element diminished the number of G418-resistant cell foci, when plasmids containing the neo marker gene under the control of E1a promoter were used. Plasmid stability in transfected cells was not modified by deletion of this cis-acting negative element.

Adenoviridae↗
PubMed1987

[Granulomatous lesions of the breast. Their role in inflammatory breast pathology and their relations to lobular granulomatous mastitis].

The authors summarise the clinical notes and histological findings of 17 patients who had inflammatory changes in their breasts and in whom biopsies showed epithelioid granulation tissues with giant cells. Three separate histological tables have been prepared as well as a review of the literature. The paper shows that mixed granulomatous conditions exist, with the sites of the changes being both in the canals and in the lobules. This gives rise to a discussion about the separateness of the condition, which is presumed to be of an autoimmune origin. It is pointed out that there can be an infectious element present.

Abscess↗
PubMed1984

Characterization of an early temperature-sensitive and cytocidal double mutant of adenovirus type 2.

Human adenovirus type 2 mutant, H2 ts 111, presented a double phenotype: temperature-sensitive (ts) for initiation and elongation of DNA synthesis, and cytocidal (cyt) by its large-plaque formation and the nucleolytic cleavage of both viral and cellular DNAs. Both characters were recessive since they were efficiently complemented by wild-type or other DNA-negative ts mutants. H2 ts 111 DNA--terminal protein complex formed at 33 degrees C and chased at 39.5 degrees C showed a decreased affinity for glass fibre filters, concurrently with the loss of protein-linked DNA ends. H2 ts 111 DNA breakdown occurring upon shift-up to 39.5 degrees C therefore appeared to start in close proximity to the genome extremities. Marker rescue experiments showed that the ts character was abolished by co-infection with plasmid recombinants containing whole or part of the E2A region, encoding for the 72K DNA-binding protein. The N-terminal domain of this 72K protein has been assigned between 0 and 200 amino acids, and is supposed to have a function in late transcription control. Since H2 ts 111 mapped between 0 and 300 residues (63.6 to 65.9 map units), its mutation was most likely located between 200 and 300 amino acids, namely in the C-terminal domain of the protein, which is involved in DNA replication. Recombination between H2 ts 111 and H5 dl 313 mutant revealed that the cyt function was localized in the E1B zone, between 3.8 and 11.3 map units. The nucleolytic and cytocidal effects were complemented by HEK 293 cells, an H5-transformed cell line expressing the left-most 12.5% of the viral genome. H2 ts 111 appeared, therefore, as a double ts-cyt mutant. The gene product rendered temperature-sensitive by the H2 ts 111 mutation was found to act stoichiometrically, and not catalytically, a result compatible with a lesion in the 72K protein. Although inactive at the non-permissive temperature, the early 72K protein was normally synthesized and stable in H2 ts 111-infected cells at 39.5 degrees C. Assymetric complementation obtained with the DNA-defective H5 ts 36 implied a certain degree of type specificity in the DNA-binding protein function.

Adenoviruses, Human↗
PubMed1982

Physical mapping of adenovirus type 2 temperature-sensitive mutations by restriction endonuclease analysis of interserotypic recombinants.

The genome structures of about 100 interserotypic ts recombinants produced in crosses between human adenovirus type 2 (H2) and 5 (H5) temperature-sensitive mutants were analyzed by cleavage with restriction endonucleases to determine the map coordinates of the following temperature-sensitive mutants: penton base plus fiber-defective H2 ts103, -104, and -136, assembly-defective H2 ts112, fiber-defective H2 ts125, hexon-defective H2 ts118 and -121, and DNA-negative H2 ts111. H5 ts1 (100 K defective), H5 ts36 (DNA negative), H5 ts125 (mutated in the early 72,000-dalton protein), H5 ts22 (fiber defective), H5 ts58 (IIIa defective), and H5 ts18 and -19 were used as one of the parents. The physical locations of the H2 temperature-sensitive mutations thus defined are discussed in relation to the genetic map, the biological function altered, and the positions of the structural genes on the genome.

Adenoviruses, Human↗
PubMed1982

[Cystadenocarcinoma complicating urachal cysts. A case report and review of the literature (author's transl)].

A 50-year-old man developed a cystadenocarcinoma on a large urachal cyst. Only 15 similar cases have been reported in the published literature, equivalent to less than 10 p. cent of cancers of the urachus. These tumours present certain clinical, radiological, endoscopic, ultrasonographic, and pathological characteristics. As against solid urachal tumours, there is little controversy as to their vestigial origin from the remains of the allantoid canal. Their prognosis is poor, regional recurrences and pulmonary metastases, and complicated or uncomplicated urachal cysts should therefore be systematically excised.

Cystadenocarcinoma↗
PubMed1981

[Multiple cystadenolymphomas of the parotids and upper lip (author's transl)].

Cystadenolymphomas occur fairly frequently in the salivary glands, usually as a single tumour in one parotid. Bilateral and multiple forms have also been reported, and their presence in other principal or accessory salivary glands has been described. It is exceptional, however, for them to occur simultaneously or successively as multiple lesions in the principal or accessory salivary glands. A case is reported of a 46-year-old man presenting with multiple cystadenolymphomas in both parotid glands and the mucosal glands of the upper lip.

Adenolymphoma↗
PubMed1980

[Teratoma of the neck. A report of one case (author's transl)].

The authors present a rare case of teratoma developed in the cervical region of a female teenager. Comparing this case to the literature, they study the clinical aspects which are different according to the age of the discovery, pointing out the most frequent case of the newborn and they study the histological findings setting out the diagnosis. Prognosis depends on the maturity degree of the tissues; it is more often than not excellent after surgical exeresis with child contrary to the seldom adult cases of which the evolution is quickly distressing.

Adolescent↗
PubMed1979

Adenovirus early function required for protection of viral and cellular DNA.

Studies were done to characterize a DNA-negative temperature-sensitive (ts) mutant of human adenovirus type 2, H2 ts111. The temperature-sensitive defect, which was reversible on shift-down in the absence of protein synthesis, was expressed as early as 2 h postinfection, and the results of density-labeling experiments are in agreement with at least a DNA replication initiation block. On shift-up, after allowing viral DNA synthesis at permissive temperatures, the newly synthesized viral DNA and the mature viral DNA were cleaved into fragments which sedimented as a broad peak with a mean coefficient of 10-12S. This cleavage was more marked in the presence of hydroxyurea as the DNA synthesis inhibitor. Parental DNA in infected cells was degraded to a much lesser extent regardless of the incubation temperature. In contrast, the parental DNA was strongly degraded when early gene expression was permitted at 33 degrees C before shift-up to 39.5 degrees C. Furthermore, cellular DNA was also degraded at 39.5 degrees C in ts111-infected cells, the rate of cleavage being related to the multiplicity of infection. This cleavage effect, which did not seem to be related to penton base-associated endonuclease activity, was also enhanced when early gene expression was allowed at 33 degrees C before shift-up. The ts111 defect, which was related to an initiation block and endonucleolytic cleavage of viral and cellular DNA, seemed to correspond to a single mutation. The implication of the ts111 gene product in protection of viral and cellular DNA by way of a DNase-inhibitory function is discussed.

Adenoviruses, Human↗
PubMed1979

[Contribution of two-dimensional immunoelectrophoresis technics to the study of the structure and the morphogenesis of human adenovirus].

The two-dimensional immunoelectrophoresis has been applied to human adenovirus type-2 structural analysis. This method is both qualitative and quantitative and was used in the following studies. 1) Detection of major and minor capsid antigens. 2) Identification of viral antigenic components in the infected cell material. 3) Quantification of antigens and serological characterization of ts mutants. 4) Identification of polymeric forms of structural proteins. 5) Assembly analysis of capsid subunits. 6) Stoichiometric analysis of proteins within the virion. 7) Obtaining of monospecific sera.

Adenoviruses, Human↗
PubMed1978

Isolation and phenotypic characterization of human adenovirus type 2 temperature-sensitive mutants.

Thirty-nine temperature-sensitive (ts) mutants that fail to grow at 39.5 degrees C but develop normally at 33 degrees C have been isolated from a nitrous-acid-treated stock of a wild-type strain of type 2 human adenovirus. The frequency of ts mutants among the surviving viruses was about 10%. Complementation tests in doubly infected cell cultures at restrictive temperature permitted the assignment of 19 of these mutants to 11 complementation groups. They are characterized phenotypically according to their soluble capsid antigen production quantified by two-dimensional immunoelectrophoresis, virus DNA synthesis, as measured by alkaline sucrose gradient sedimentation of 34S DNA, and virion morphogenesis, as analysed by electron microscopy os cell sections. Two complementation groups were defective for DNA synthesis, four for soluble hexon production and two groups for total penton (penton base + fibre), while one group revealed no fibre production. Two complementation groups presented a normal antigen pattern, but the particles exhibited altered morphology as observed in cell sections.

Adenoviruses, Human↗
PubMed1976

[Intrapericardial congenital ectasias of the left atrium].

Congenital intrapericardial dilatation of the left atrium is an abnormality which is either very rare (16 cases reported in the literature) or one that is easily missed. The clinical features are unhelpful, and the diagnosis is suggested by the outline on X ray and confirmed by angiocardiography. The great importance of this abnormality lies in its natural history. Disorders of rythm (9 cases out of 16) and embolism (5 cases out of 16) are amongst the complications to be weighed up when deciding the treatment policy. Operative treatment, which is straightforward, seems to us to be indicated in all cases.

Arrhythmias, Cardiac↗
PubMedDate not supplied

[Ascariasis of the cervix].

The authors report a case of ascaris lumbricoïdes located in the cervix uterus of a patient suffering from gonococcal salpingitis. Reviewing the literature, they conclude that the most likely way to the parts genitals was a transanal migration toward vagina.

Adult↗