A new method for the evaluation of reaction kinetics of immobilized enzymes investigated in single enzyme--Sepharose beads by microfluorometry.
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Animals↗
Biomedical subjects
C Couwenbergs
Publications and source records attributed to C Couwenbergs.
Fluorogenic substrate turnover in single living cells.
Intracellular diffusion properties and enzyme activities in single living cells can be analysed by means of fluorogenic substrates that diffuse into the cells where they are converted into a fluorescent product by an enzymic reaction. The reaction-kinetic analysis of this process as a system of consecutive reactions provides information on the diffusion of the substrate into the cells, on intracellular enzyme activities and on the efflux of the fluorescent product. Separation of diffusion and enzyme-mediated processes is obtained by inducing specific changes of the cellular membrane using gramicidin D. A model for the functional interpretation of the experimental findings is proposed. Application of this method as a viability test for freshly prepared and frozen platelets is discussed.
Blood Platelets↗
[Effect of cromoglicinic acid on the degranulation of mast cells (author's transl)].
An interference microscopic method is described which allows quantitative measurements of the inhibiting effect of the di-sodium salt of 1,3-bis(3-carboxy-chroman-5-yl-oxy)-2-hydroxypropand (cromoglicinic acid; Intal; DSCG) on the degranulation of mast cells in vitro induced by polymixin B and Compound 48/80. The specific degranulation inhibiting effect of DSCG is increased in the presence of Ca++ and Mg++ ions.
Animals↗