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C Cuppini

Publications and source records attributed to C Cuppini.

26 records · Page 2Linked to original sources
PubMed1985

[Recovery of sensation and reflex activity during treatment with ethanol].

Ethanol greatly modifies synaptic function and it affects the reinnervation time course. In order to better clarify the effects of ethanol on nerve regeneration, we have observed the recovery of algesthesia and reflex activity in the rat during the treatment with ethanol. For this purpose the posterior leg of Sprague Dawley rats was denervated by crushing the sciatic nerve and the recovery of algesthesia and reflex activity was tested at the level of the metatarsus-falanx articulation of the homolateral paw. During the reinnervation period, the animals were treated daily with ethanol 3 g/Kg of body weight. The recovery of algesthesia and reflex activity comes about in a medio-lateral direction and proceeds linearly. It is completed on the 25th day after the denervation. Ethanol does not cause changes in the time course of algesthesia recovery, however it does cause a slight delay in the recovery of the reflex activity.

Animals↗
PubMed1984

[Changes in the synaptic activity in sciatic nerve-extensor digitorum longus (preparations induced by ethanol)].

The aim of this research is the study of the modification of synaptic activity caused by ethanol in the rat sciatic nerve-extensor digitorum longus (EDL) muscle preparation. For such a purpose, intracellular recordings have been carried out, keeping the muscle immersed in normal Ringer solution and in Ringer solutions containing ethanol at different concentrations up to 0,8 M. Therefore, the resting potential of muscle cells and the frequency of m.e.p.p.s were measured. Qualitative observations of m.e.p.p.s shape were also carried out. Ethanol increases the frequency of m.e.p.p.s in the rat sciatic nerve - EDL muscle preparation. The logarithm of relative frequency (frequency in Ringer solution with ethanol/frequency in normal Ringer solution) is linear with respect to the concentration of ethanol, with a slope of 1.44. Furthermore, ethanol increases the amplitude and lengthens the time course of m.e.p.p.s. The muscle cells undergo a hyperpolarization of about 2-3% at the lowest concentrations of ethanol tested.

Animals↗
PubMed1984

[Variations in the hyperpolarization potential of ethanol on muscle during reinnervation].

Ethanol causes the hyperpolarization of the excitable membranes. In the Extensor Digitorum Longus (EDL) muscle of the rat the increase of resting membrane potential is 2-5% and is independent of the concentration of alcohol between 0.2 and 0.4 M, while at higher concentrations the membrane potential falls to levels equal or inferior to the normal potential. We have studied the hyperpolarization action of ethanol on the denervated muscle by crushing the sciatic nerve. Also under these conditions in which, as is known, there is a drop in the resting potential, ethanol causes hyperpolarization, however it is in general greater and it is dependent upon the concentration between 0.2 and 0.8 M.

Animals↗
PubMed1984

[The recovery of motor innervation during treatment with ethanol].

We have studied the effects of the fluidizing action of ethanol on motor reinnervation in order to clarify if membrane fluidity changes affect synaptic plasticity and nerve regeneration. Sprague Dawley rats were denervated by crushing the sciatic nerve and subsequently, on the 16th day, the degree of reinnervation of the EDL muscle was observed by electrophysiological technique: in particular an observation was made of the resting potential and the m.e.p.p.s frequency by intracellular recordings in muscle fibers. During the nerve regeneration period, the rats were treated with 3 g of ethanol per kilogram of body weight per day. We have found that ethanol quickens the resting potential recovery but does not affect the m.e.p.p.s frequency.

Action Potentials↗
PubMed1978

Glucokinase and hexokinase in pig erythrocytes.

We have shown different enzymatic activities responsible for the phosphorylation of glucose in pig erythrocytes. These activities were observed after partial purification from hemolyzed red cells. One of the enzymes involved is the hexokinase which is present in all tissues; the other is similar to hepatic glucokinase. We have determined the kinetic properties of these activities in hemolysates and in partially purified preparations. Their electrophoretic-migration characteristics were studied too.

Animals↗
PubMedDate not supplied

Motor nerve sprouting induction by a nerve explant in normal and vitamin E-deficient rats.

Sprouting induction by a peripheral degenerating nerve has been evaluated in normal and vitamin E-deficient rats. A piece of sural nerve was grafted close to the peroneal nerve of the same animal: at one and two months after grafting thin unmyelinated axons were visible in the graft and they were sometimes functionally active; when nerve explant was frozen before grafting, sprouting induction did not take place either in controls or in vitamin E-deficient animals. No difference was noted in sprouting induction between the two groups, while degeneration showed a different time course. Some hypotheses of possible stimuli of sprouting induction are discussed.

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