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Biomedical subjects

C D Crisci

Publications and source records attributed to C D Crisci.

29 records · Page 2Linked to original sources
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The use and limitations of laser nephelometry.

The quantitative determination of several proteins in serum and liquor received new impulses with Laser Nephelometry. The method is characterized by its speed, precision, accuracy, high sensitivity, good correlation when compared with radial immunodiffusion and the possibility of automatization. The detection of mycotic antigens and precipitating antibodies against organic dusts and against DNS and the detection of immune complexes show the flexibility of the new method.

Antibodies↗
PubMedDate not supplied

Immune complexes in epidemic (type A) hepatitis. Detection by three methods using laser nephelometry.

Thirty-eight children with acute epidemic hepatitis (Type A) and 22 children in "contact" with the disease, 5 of whom subsequently developed hepatitis, were studied. Three technics based on laser ray nephelometry were employed to investigate the presence of circulating immune complexes. Our results indicated that circulating immune complexes are present in the serum during the course of epidemic hepatitis. These were demonstrated in 79% of the patients. The capacity to fix complement diminishes as the disease evolves toward the convalescent phase. We also detected a similar percentage of immune complexes (80%) in the patients during the incubation period. In the subjects in "contact" with the disease who subsequently did not develop hepatitis, the immune complexes were shown to have a greater capacity to fix complement, and the percentage of immune complexes present was higher (94%). The specificity of the immune complexes was verified by correlating the antivirus A antibody activity with the percentage of C4 in the precipitates obtained by polyethylene glycol (3.5%).

Adolescent↗
PubMedDate not supplied

Immunological aspects of sarcoidosis associated with true thymic hyperplasia.

A 34-year-old woman had a three-month history of a double mitral lesion. Chest radiographs disclosed a mediastinal mass. Thoracotomy was performed and multiple white nodules and an enlarged thymus were seen. Sarcoidosis and true thymic hyperplasia were diagnosed in the excised white nodules and thymus. A decrease of cell-mediated immunity was also discussed. When commissurotomy of the mitral valve was performed 4 months later, sarcoidosis and true hyperplasia had disappeared and alterations of cell-mediated immunity became normal after a course of steroid therapy. The alterations of cell-mediated immunity, so frequent in sarcoidosis, could be related to true thymic hyperplasia.

Adult↗
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Evaluation of total IgE in diverse allergosis. Comparative study with other techniques. Part II.

A total of 181 allergic patients were studied and classified according to the causal antigen, 92 patients were sensitized to the mixed grass pollen, 55 to house dust, 28 to Candida albicans, 31 patients with egg allergy and 25 with milk allergy. All of them presented strong positivity to anamnesis, intracutaneous tests and exposition tests. In all of them the serum total IgE was determined using the radioimmunoassay (RIST) and the antigen specific IgG, following Boyden's passive hemagglutination technique. In 31 patients with pollinosis, 55 with house dust allergy and 25 with sensitization to Candida albicans, the determination of the antigen specific IgG was also carried out following the quantitative precipitation method. The results showed a greater reliability on the hemagglutination as opposed to the determination of total IgE with grass pollen. The hemagglutination was positive in 90.20% and the total IgE was increased in 59.78% of the cases. In patients with house dust allergy the total IgE was elevated in 69.08% of the cases and the hemagglutination was increased in 60.71% of the cases. In those cases of sensitivity to Candida albicans, the total IgE presented higher values in 63.84% of the cases, while the hemagglutination was increased in 21.43% of the cases. In egg allergy,, the IgE was increased in 56.83% of the cases and the hemagglutination in 87.09% of all of the 31 cases studied. In the cases of milk allergy the IgE presented increases in 32% of the cases and the hemagglutination gave higher titers in 56% of all of the 25 cases studied. The results make evident that the hemagglutination should be included in the rutinary diagnosis of allergic diseases as well as the determination of total IgE or antigen specific IgE.

Adult↗
PubMedDate not supplied

The cellular immunity in patients sensitized to Candida albicans and its corresponding humoral response.

Ten non-sensitized normal blood donors and thirty five patients with a strongly positive intracutaneous test to Candida albicans, positive anamnesis to Candida allergy, or with an actual Candida infection demonstrated by positive cultures of the focus, were studied. In all of them, an evaluation of the humoral and cellular response to Candida antigens, was performed both in vivo and in vitro. The results obtained in the tests performed in the control group were considered as extreme values of a normal response and taken as reference in order to evaluate the patients' response. The results of the test performed on the 35 patients were processed in an IBM S.370/M.125 computer and submitted to a General Taxonomy Program in order to group the patients according to their characteristics and similar behaviour. Two main groups, each one including three minor groups, were obtained and only one individual was not classified. According to this classification it was possible to distinguish two immunological patterns: a) The patients that had a normal or increased percentage of T lymphocytes were capable of responding adequately to Candida albicans antigens. They showed positive immediate and delayed skin tests, increased hemagglutination titres, increased lgE values, and their lymphocytes were capable of undergoing positive blast transformation. Those patients from this group who had a candidaemia at the time of the study, reached the highest hemagglutination values and presented precipitin antibodies as a characteristic. b) The patients that had a decreased percentage of T lymphocytes presented a partial deficiency in their response to Candida albicans antigens. They showed negative delayed skin tests; hemagglutination and precipitation tests both negative, normal lgE values and their lymphocytes did not undergo blast transformation. These findings suggest that a normal percentage of T cells is required in order to obtain a suitable cellular response against Candida albicans, and that an appropriate co-operation between T and B cells is also necessary in order to obtain a good humoral response to Candida antigens.

Antibody Formation↗