PubMed Health⌕ Search

Biomedical subjects

C D Lytle

Publications and source records attributed to C D Lytle.

At least 55 records · Page 3Linked to original sources
PubMed1976

Radiation enhanced reactivation of herpes simplex virus: effect of caffeine.

Ultaviolet enhanced (Weigle) reactivation of UV-irradiated herpes simplex virus in UV-irradiated CV-1 monkey kidney cell monolayers was decreased by caffeine. X-ray enhanced reactivation of UV-irradiated virus in X-irradiated monolayers (X-ray reactivation) and UV- or X-ray-inactivated capacity of the cells to support unirradiated virus plaque formation were unaffected by caffeine. The results suggest that a caffeine-sensitive process is necessary for the expression of Weigle reactivation for herpes virus. Since cafeine did not significantly affect X-ray reactivation, different mechanisms may be responsible for the expression of Weigle reactivation and X-ray reactivation.

Caffeine↗
PubMed1976

Infection of UV-irradiated xeroderma pigmentosum fibroblasts by herpes simplex virus: study of capacity and Weigle reactivation.

The capacity of monolayers of both normal human and xeroderma pigmentosum (XP) filbroblasts to support plaque formation by herpes simplex virus was decreased when the monolayers were ultraviolet (UV) irradiated and infected with virus. Fibroblasts of XP complementation groups A, B, and D were sensitive to UV, being 4-6 fold more sensitive than either fibroblasts of XP complementation group C or fibroblasts from a normal individual. When the monolayers were irradiated 4 days prior to infection, the capacity of normal fibroblasts to support herpes virus growth recovered, whereas the capacity of the XP strains decreased further compared to that measured when infection immediately followed irradiation. Concurrent experiments with UV-irradiated herpes virus showed that the survival of this virus did not increase when infection by irradiated virus immediately followed irradiation of the monolayers. However, if the monolayers were irradiated 4 days prior to infection, the survival of this virus increased by a factor of nearly 2. Such Weigle reactivation (WR) occurred at lower fluences to the XP fibroblasts than to normal fibroblasts, suggesting that WR results from residual cellular DNA damage left after excision repair.

Cell Line↗
PubMed1971

X-ray-enhanced reactivation of ultraviolet-irradiated human virus.

When CV-1 mammalian cells were X-irradiated before infection with ultraviolet (UV)-irradiated herpes simplex virus, an increase in survival of this virus was observed. X-ray reactivation is proposed as the name of this phenomenon by analogy with UV reactivation. The amount of survival enhancement was about the same as that found for UV reactivation in the same virus-host system. The enhancement reached a plateau at approximately 1000 rads and appeared to be radiation-insensitive at high doses.

Animals↗