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C D Pfaffenberger

Publications and source records attributed to C D Pfaffenberger.

23 records · Page 2Linked to original sources
PubMedDate not supplied

Cytokinetic and cytogenetic effects of some agricultural chemicals on human lymphoid cells in vitro: organophosphates.

In the present study, cytotoxic, cytostatic and cytogenetic effects of a number of organophosphate pesticides on human lymphoid cells (LAZ-007) in culture have been examined. Cytotoxic effects were dose related and often led to extensive cell kill. The pronounced effects of various organophosphates on the cell-cycle traverse were shown in data based on the enumeration of M1 and M3 metaphases after incubation of cells with BrdU. In cells incubated with 20 micrograms/ml of the various chemicals, the number of M1 metaphases ranged from 6% (R-1303) to 18% (Azodrin) compared to no M1 metaphases in control cultures. The number of M3s in cultures treated with 20 micrograms/ml of the various chemicals tested varied from 0% (Phosdrin) to 7% (parathion) as compared to 17% in control cultures. 11 out of the 14 organophosphates tested, significantly increased the SCE frequency. Of the 9 chemicals tested after metabolic activation by liver microsomal S9 preparation, significant increases in SCE frequency were seen in diazinon-, dimethoate-, Dursban- and Phosdrin-treated cells.

Animals↗
PubMedDate not supplied

Determination of organochlorine pesticides and metabolites in drinking water, human blood serum, and adipose tissue.

Previously published analytical procedures by which drinking water, human blood serum, and adipose tissue may be analyzed for trace amounts of organochlorine pesticides have been modified to permit use of smaller samples. Data on percent recovery, detector sensitivity, and limit of detectability have been collected for each of the three reported procedures. The methodology has been applied to 59 blood samples, 20 drinking water samples, and 10 necropsy adipose tissue samples. Only hexachlorobenzene and dieldrin were detected in water. Hexachlorobenzene, beta-hexachlorocyclohexane, dieldrin, p,p'DDE, and p,p'DDT were present in blood. No heptachlor, heptachlor epoxide, oxychlordane, trans-nonachlor, or o,p'-DDT was found. In.fat, the compounds present in blood plus oxychlordane, heptachlor epoxide, and trans-nonachlor were detected. No heptachlor, aldrin, o,p'-DDT, or alpha- or gamma-chlordane was present. The reported values are representative of the levels of persistent organochlorine pesticides and pesticide metabolites found in 1978 in white female residents of Dade County, Florida.

Adipose Tissue↗