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C D SMITH

Publications and source records attributed to C D SMITH.

17 recordsLinked to original sources
PubMed1965

CORRELATION OF FLUORESCENT ANTIBODY, HISTOPATHOLOGY, AND CULTURE ON TISSUES FROM 372 ANIMALS EXAMINED FOR HISTOPLASMOSIS AND BLASTOMYCOSIS.

Porter, Billie M. (Communicable Disease Center, Kansas City, Kan.), Barbara K. Comfort, Robert W. Menges, Robert T. Habermann, and Coy D. Smith. Correlation of fluorescent antibody, histopathology, and culture on tissues from 372 animals examined for histoplasmosis and blastomycosis. J. Bacteriol. 89:748-751. 1965.-In a survey in the midwestern United States, tissues from 372 animals including 16 species were examined for Histoplasma capsulatum and Blastomyces dermatitidis. All of the specimens were cultured and studied by use of the fluorescent-antibody (FA) technique, and histopathological studies of tissue sections were done. Among the 372 animals, 300 were negative by all three methods of examination, 34 had blastomycosis, and 21 had histoplasmosis. The remaining 17 were positive for histoplasmosis with the FA technique but were negative by histopathology and culture. An animal was considered to have histoplasmosis or blastomycosis only when the specific fungus was isolated or demonstrated in tissue sections or exudates from cutaneous lesions. Of the 34 blastomycosis cases, 25 (74%) were positive by FA, 32 (94%) by histopathology, and 11 (32%) by culture. Of the 21 histoplasmosis cases, 15 (71%) were positive by FA, 16 (76%) by histopathology, and 14 (67%) by culture. It appeared that FA, histopathology, or culture alone was insufficient for a diagnosis. All three techniques should be employed for the most satisfactory diagnostic results.

Animals↗
PubMed1964

INFECTION OF WHITE SWISS MICE WITH AIRBORNE CRYPTOCOCCUS NEOFORMANS.

Smith, C. D. (Communicable Disease Center, Kansas City, Kansas), R. Ritter, H. W. Larsh, and M. L. Furcolow. Infection of white Swiss mice with airborne Cryptococcus neoformans. J. Bacteriol. 87:1364-1368. 1964.-A group of 39 white Swiss male mice were allowed to run 3 days on previously sterilized soil that had been seeded with Cryptococcus neoformans 1 year previously. It was determined that the soil contained an average of 1.6 x 10(6) viable yeast cells per ml. The mice were observed for 24 weeks, at which time the survivors were necropsied. The total mortality rate during this period was 44%; 67% of the mice had positive cultures, including all who had a fatal infection. Two additional experiments were done with the same strain with the use of aerosols produced by a Henderson apparatus. In experiment 1, 39 mice received 11,000 viable cells each; in experiment 2, 40 mice received 14,622 viable cells. Both groups of mice showed similar mortality rates, with a total of 77% dead after 20 weeks in experiment 1, and 80% in experiment 2. All of the mice in experiment 1 had positive cultures, as did 95% of the mice in experiment 2.

Aerosols↗