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C D Souza

Publications and source records attributed to C D Souza.

5 recordsLinked to original sources

Evaluation of propofol and remifentanil for intravenous sedation for reducing shoulder dislocations in the emergency department.

OBJECTIVES: To assess the combination of propofol and remifentanil for sedation to reduce shoulder dislocations in an ED. METHODS: Eleven patients with anterior glenohumeral dislocation were given propofol 0.5 mg/kg and remifentanil 0.5 microg/kg iv over 90 seconds and then further doses of 0.25 mg/kg and 0.25 microg/kg, respectively, if needed. Another practitioner attempted reduction using the Milch technique. RESULTS: Reduction was achieved in all patients within four minutes of giving sedation (range 0.3-4; mean 1.6). Seven required one attempt at shoulder reduction, three required two attempts, and one required three attempts. Mean time to recovery of alert status was three minutes (range 1-6). The mean pain score during the reduction was 1.7 out of 10 (range 0-5). Nine patients had full recall, one had partial recall, and one had no recall at all. Eight patients were "very satisfied" with the sedation and three were "satisfied". There were no respiratory or haemodynamic complications that required treatment. CONCLUSIONS: Propofol and remifentanil provide excellent sedation and analgesia for the reduction of anterior glenohumeral dislocation, enabling rapid recovery.

Adolescent↗

Mucosal immune response in cattle with subclinical Johne's disease.

Mycobacterium avium subsp. paratuberculosis is the causative agent of Johne's disease, a chronic granulomatous enteritis of wild and domestic ruminants. During a long subclinical period, the organism persists in the intestine despite systemic cellular and humoral immune responses. To explore the mucosal immune response in Johne's disease, we isolated mononuclear leukocytes from the ileum of cows naturally infected with M. avium subsp. paratuberculosis and from cows that were not infected. We evaluated the immunophenotype of these cells and the proliferative responses after the addition of M. avium subsp. paratuberculosis sonicate or B-cell or T-cell mitogens. Although the percentage of T cells was increased in infected cows, these cells consisted mostly of memory (CD2+CD62L-) and regulatory (CD4+CD25+) T cells. Further evidence of immune hyporesponsiveness included a decrease in the percentage of T cells with an activated phenotype and a decrease in cells expressing major histocompatibility factor class II (MHC class II). Unlike the spleen, ileal lymphocytes from infected cows failed to proliferate in response to M. avium subsp. paratuberculosis sonicate. Additionally, ileal lymphocytes from infected cows proliferated poorly in response to concanavalin A and pokeweed mitogen, suggesting generalized T cell and B cell hyporesponsiveness. These results indicate that a state of tolerance may exist in the intestine of cows subclinically infected with M. avium subsp. paratuberculosis organisms in subclinically infected cows. This effect may be induced, at least in part, by proliferation of regulatory T cells that nonspecifically suppress mucosal immune responsiveness.

Animals↗

[Congenital hypothyroidism screening program: preliminary results].

OBJECTIVE: The authors report early results of a screening program for Congenital Hypothyroidism in Santa Catarina, Brazil. METHODS: All the assays (82.709) analysed by Laboratório Central (LACEN) between July 1993 and December 1994 were studied. A blood sample was collected on filter paper for serum Thyroid Stimulating Hormone (TSH) measurement by fluoroimmunoassay, in triplicate. All children who had persistent abnormal TSH values (cord blood: TSH > 30 micro U/ml; from 2 to 7 days: >20 micro U/ml; older than 7 days: > 10 micro U/ml) were recalled for clinical evaluation. RESULTS: Out of 82.709 infants aged from 0 to 60 days (40% of the deliveries in this period), 304 presented abnormal results of TSH and 34 were confirmed as Congenital Hypothyroidism. The mean age of beginning the treatment was 45 days of life. CONCLUSIONS: The prevalence (1:2500) was higher than that observed in other countries, probably because some children had Transient Congenital Hypothyroidism. Nevertheless, sex prevalence was similar to that described in the literature. Clinical manifestations were inespecific which reinforces the importance of this kind of screening. Efforts have been done to improve the program in order to begin earlier treatment.

English Abstract↗