Biomedical subjects

C D'Arcangelo

Publications and source records attributed to C D'Arcangelo.

At least 19 recordsLinked to original sources

PubMed2005

Changes of plasma membrane properties in a human pre-T cell line undergoing apoptosis.

A variety of cellular functions are modulated by the physical properties of the cell membrane, and the modification of intracellular transfer, resulting from loss of membrane integrity, may contribute toward setting the cell onto the pathway of apoptosis. Apoptosis in lymphoid cells can be induced in different ways and biochemical modifications occur at an early phase of cell death, while the morphological features of apoptosis are evident later. We previously reported that DMSO is an efficient apoptosis-inducing factor in the human RPMI-8402 pre-T cell line. The aim of the present study was to verify the effect of DMSO on the plasma membrane fluidity, the intracellular calcium concentration and the phosphodiesterase activity in DMSO-induced apoptosis. Our results show a modification of membrane fluidity associated with an increase of intracellular Ca(2+) concentration. Moreover, we demonstrate that these modifications are related to a decrease in the phosphodiesterase (PDE) activity. The correlation between the proceedings of added DMSO and the induction of apoptosis will provide significant information regarding the first part of the apoptotic process.

Apoptosis↗

PubMed2004

Nitric oxide synthase in healthy and inflamed human dental pulp.

Nitric oxide synthase (NOS) plays a significant role in the pathogenesis of pulpitis. In this study, we hypothesized the existence of endothelial (eNOS) and inducible (iNOS) enzyme isoforms in human dental pulp. Extracted third molar pulps were divided into groups based on clinical diagnosis: healthy, hyperemic, and irreversible pulpitis. We have localized the eNOS and iNOS by immunohistochemistry and have tested their mRNA expression by RT-PCR and protein levels by Western blots. eNOS is present in the endothelial cells and odontoblasts of the healthy pulp, but an elevation of eNOS mRNA and protein levels with a concomitant dilation of vessels was characteristic under pathological conditions. Healthy pulp tissue failed to exhibit any iNOS; however, acute inflammation enhanced the mRNA and protein levels of iNOS, mainly in the leukocytes. There are differences in localization and expression between eNOS and iNOS in healthy and inflamed dental pulp.

Adult↗

PubMed2004

Microbiological study and scanning electron microscopic analysis of root canal wall dentin following pumped Diodium Nd:YAG laser irradiation.

The capability of Nd:YAG laser in sterilizing root canals and the alterations of dentinal walls induced by laser treatment were investigated. Thirty root canals were infected by P. aeruginosa ATCC 27853 and thirty canals by A. naeslundii CH-12. Within each infection, 4 groups were selected on the basis of the treatment. Among them, 2 test groups (TGs) were treated by Nd:YAG laser at 15 Hz for 15 s, using 2 different settings: 1 Watt/70 Joule and 1.5 Watt/100 Joule, respectively (n = 10 each). The other 2 groups, used as controls (CGs), were: untreated (positive control, n = 5) and sterilized by 5.25% NaClO group (negative control, n = 5). Observations under scanning electron microscope (SEM) and quantitative bacterial counts were performed. These analyses were performed once per group after infections and treatments. Laser treatments significantly reduced the number of both bacteria. SEM investigation showed melting and crystallization of canal dentin over 1.5 W/100 J. Laser irradiation has a bactericidal effect but it does not completely sterilize the root canal as NaClO 5.25% solution does if the goal of treatment is also to avoid alterations of dentinal walls.

Actinomyces↗

PubMed2001

Root canal treatment in mandibular canines with two roots: a report of two cases.

AIM: To highlight anatomical variation in the mandibular canine. SUMMARY: Two clinical case reports are presented to illustrate anatomical variation in the human mandibular canine. Endodontic treatment may sometimes fail because morphological features of the tooth adversely affect the treatment procedures. Many investigators have reported the anatomical variations associated with mandibular canines. Mandibular canines are recognized as usually having one root and one root canal in most cases, although approximately 15% may have two canals or sometimes two roots. This paper describes two clinical cases of mandibular canines with two roots and two canals. KEY LEARNING POINTS: Failure to control infection in the root canal system has an adverse impact on outcome. Clinicians should be aware of anatomical variations in the teeth they are managing, and should never assume that canal systems are simple. The majority of mandibular canines have one root and root canal, but 15% may have two canals, and a smaller number may have two distinct roots.

Cuspid↗

PubMed2000

Localization of the e-NOS enzyme in endothelial cells and odontoblasts of healthy human dental pulp.

Nitric oxide synthases (NOS) are important enzymes present in different cells such as endothelial cells, macrophages, etc. Recently, it has been found that nitric oxide (NO) is responsible for vasodilation, blood pressure regulation, platelet aggregation, cardiac contractility, and the mediation of immunity during bacterial infections and inflammation. However, the production and role of NO in various structures of the oral cavity have not been investigated extensively. The aim of this study was to evaluate the presence of e-NOS in healthy human odontoblasts and endothelial cells of the dental pulp. Twenty healthy human dental pulps were collected and frozen and pulp slices were obtained using a cryostat. The e-NOS enzyme was revealed by immunohistochemical analysis and the enzyme level was detected by Western blotting and mRNA expression by RT-PCR. The immunohistochemical results demonstrated, for the first time, the presence of e-NOS in odontoblasts and in endothelial cells. The presence of e-NOS m-RNA was confirmed by RT-PCR and the expression of the protein by Western blotting. These results clearly show that the e-NOS enzyme is present in both odontoblasts and endothelial cells of healthy human pulp. The presence of e-NOS in the odontoblast and endothelial cells of the dental pulp may mediate local vasodilation and cell proliferation.

Blotting, Western↗

PubMed2000

Broken instrument removal--two cases.

This paper reports two clinical aids for the removal of broken instruments in root canals using a combination of hand instrumentation and ultrasonic devices. This technique is based on two phases: hand instrumentation with stainless-steel K-file instruments for bypassing the fragments, and K-files mounted on an ultrasonic handpiece for loosening and retrieval of the broken instruments. This method offers the following advantages: conservation of the remaining dentin wall of the root canal and elimination of the necessity for surgical treatment.

Adult↗

PubMed2000

Canal filling in primary teeth. Description of two particular cases.

Endopedodontics is that branch of child dentistry that regards pulp treatment in childhood. It involves endodontic treatment of all primary teeth and incisives, and of the first permanent molars with immature apex. The pedodontist has to exactly evaluate the techniques and the material to use in treating his patients. It is important to realize that we are facing a continuously evolving and absolutely unstable dentition. Therefore, the success of our work is the result of a careful evaluation of diagnostic parameters and of the techniques and the material. In the current clinical cases it is obvious how the lack of only one of the parameters listed, could represent the cause of severe damage to the patient's dental health and it could lead to psychological discomfort (if not damage), considering the age of the patient.

Child↗

PubMed1999

An evaluation of the action of different root canal irrigants on facultative aerobic-anaerobic, obligate anaerobic, and microaerophilic bacteria.

The aim of this study was to test the effect of different concentrations of sodium hypochlorite, chlorhexidine, and cetrimide on the following bacterial strains: Facultative aerobes-anaerobes: Candida albicans ATCC 10231; Enterococcus faecalis ATCC 29212; Escherichia coli ATCC 25,922; Pseudomonas aeruginosa ATCC 27,853; Streptococcus mitis ATCC 9811; Streptococcus mutans ATCC 35668; Streptococcus salivarius ATCC 13419; and Streptococcus sanguis ATCC 10556. Microaerophiles: Actinobacillus actinomycetemcomitans ATCC 29522. Obligate anaerobes: Actinomyces odontolyticus ATCC 17929; Fusobacterium nucleatum ATCC 25,586; Porphyromonas gingivalis ATCC 33277; and Prevotella melaninogenica ATCC 25845. Each irrigant was kept in contact with the bacterial species used for the experiment for 10, 20, or 30 min. Results showed that all irrigants had a bactericidal effect on both facultative aerobes-anaerobes and on microaerophilic and obligate anaerobic strains, in all concentrations and even after short periods of contact.

Anti-Infective Agents, Local↗

PubMed1998

[Alpha-hemolytic streptococci and root canal irrigants. An evaluation of the bactericidal efficacy of sodium hypochlorite and chlorhexidine gluconate plus cetrimide].

BACKGROUND AND AIMS: The main bacterial species present in pulpal and periapical microbic flora is alpha-hemolytic streptococci. They are regarded as facultative anaerobes which prefer to grow in anaerobiosis. Canal irrigation plays an important role in the success of endodontic treatment given that, on the one hand, it encourages the gradual elimination of the smear layer, and on the other it neutralises microbic flora in the root canal. The aim of this study was to test the microbiological efficacy of sodium hypochlorite 1% and s new generation irrigant based on chlorhexidine 0.2% and cetrimide 0.2%. METHODS: The test was performed on the following alpha-hemolytic streptococci bacteria (Dasit, Cornaredo, Italy): Streptococcus mitis ATCC 9811; Streptococcus mutans ATCC 35668; Streptococcus salivarius ATCC 13419; Streptococcus sanguis ATCC 10556. The working concentration (CFU/ml) was defined as 0.5 Mc Farland which corresponds to a concentration of microorganisms of approximately 1.5 x 10(8) bacteria. The following canal irrigants were used: 1) cetrimide 0.2% + chlorhexidine 0.2% (Cetrexidin Vebas, S. Giuliano Milanese, Italy); 2) NaOCl 1% (Ogna, Milan, Italy). Each individual substance remained in contact with the bacterial species used in the test for 10'-20'-30'. RESULTS: The results obtained show the bactericidal efficacy of both the irrigants used, even after a short period of contact. CONCLUSIONS: This does not mean that all irrigants are equal and/or promise the same results. This was a microbiological study, but it is nonetheless important to take other variables into account, such as contact time. Moreover, in order to increase the probabilities for the success of endodontic treatment, canal irrigants must also present other characteristics, namely: biocompatibility, scarce toxicity, high proteolytic power.

Anti-Bacterial Agents↗

PubMed1998

[A comparative in-vitro study of the bactericidal efficacy of sodium hypochlorite and chlorhexidine gluconate plus cetrimide on root canal anaerobic bacterial flora].

INTRODUCTION: The permanence of necrotic tissues and/or bacteria may be responsible for persistent root canal infection. Root canal irrigation plays an important role in the success of endodontic treatment given that, on the one hand, it encourages the progressive elimination of the smear layer and, on the other, it neutralises microbic flora within the canal. The aim of this study was to test root canal irrigants from a microbiological point of view: sodium hypochlorite 5% (Niclor Ogna, Milan) and a new generation irrigant based on chlorhexidine gluconate and cetrimide (Cetrexidine VEBAS S. Giuliano Milanese-MI). METHODS: The experiments were performed on a series of obligatory anaerobic bacteria which are most frequently found in the endodontium, all belonging to the international collection "American Type Culture Collection" (ATCC) and supplied by Dasit SpA, Cornaredo (MI). The following bacterial strains were used in the experiment: Actinimyces odontolyticus ATCC 17929, Fusobacterium nucleatum ATCC 25586, Prevotella melanginogenica ATCC 25845, Porphyromonas gingivalis ATCC 33277. The working concentration (CFU/ml) was defined as 0.5 McFarland which corresponds to a concentration of microorganisms equivalent to approx. 1.5 x 10(3) bacteria. Each irrigant was kept in contact with the bacterial species used for this experiment for 10', 20', 30'. RESULTS: The results obtained confirm the bactericidal efficacy of both irrigants used, even after a short contact time. CONCLUSIONS: This does not mean that all irrigants are the same and/or promise the same results. This was a microbiological study, but in practice it is important to bear in mind other variables, such as contact time. Moreover, in order to increase the probability of endodontic treatment, root canal irrigants are also required to satisfy other criteria: biocompatibility, scarce toxicity, high proteolytic power.

Anti-Bacterial Agents↗

PubMed1997

[Epidemiologic evaluation of dental and periodontal disorders in a sample of children active in sports].

BACKGROUND: Caries and paradontal diseases occupy an important position among the various diseases of the organism. The spread of these pathologies entails consequences of an aesthetic, functional and economic nature. The aim of this study was to evaluate the oral health conditions of an age-matched and sex-matched sample population with a uniform socioeconomic background all of whom played sports. MATERIALS AND METHODS: The age groups taken into consideration are those specified by the WHO: 8-9 years old and 12-13 years old. A total of 684 children were examined within the framework of medical checkups for fitness to play sports. The epidemiological indices specified by the WHO were used to assess oral health conditions: dmf-t; DMF-T; plaque index (PLI) and paradontal index of need for paradontal treatment in community (CPITN). RESULTS: By comparing the results of this study with the targets set by the WHO for the year 2000 and with those of other studies it was seen that higher values were recorded. CONCLUSIONS: This discrepancy may probably be correlated to the marked consumption of super-calorific substances during the day by children playing sports.

Adolescent↗

PubMed1996

[A proposed clinical method for the finishing of composite-resin restorations by means of diamond burs and abrasive disks and strips: a SEM analysis].

INTRODUCTION: Composite resin is the most cosmetic material used in restorative dentistry. Its functional and cosmetic features are enhanced by an appropriate finishing. A non finishing surface is very rough, allowing therefore plaque accumulation and pigments deposition deriving from feeding. MATERIALS AND METHODS: In this study the authors performed several finishing methods using diamond burs, abrasive disks and abrasive strips. All the samples were analyzed by means of SEM at 100x, 300x and 500x. RESULTS AND DISCUSSION: The results showed several differences among tested samples according to the material and the technique used. Satisfactory finishing is performed by the association of medium and fine sizes finishing burs (Intensiv 50/D4 and Intensiv 40/D4) and medium and fine abrasive disks (Sof Lex Pop On 3M 1981 M and F), or by medium and fine finishing abrasive burs and abrasive strips (Sof Lex 3M Coarse-Medium/Aluminum Oxide N. 1954-150 and Fine-Superfine N. 1956-120).

Composite Resins↗

PubMed1996

[Legal medicine and dentistry].

With reference to cases of American dentists sued for "malpractice" during the 80s, the authors summarise the causes why, initially, the American lawyers found it difficult to plead on behalf of their clients. Therefore, the authors suggest a number of measures in order to reduce the probability of being sued by patients. In particular, they focus attention on:--the duty to treat patients, with reference to experimentation on humans within the context of scientific research and citing the Declaration of Helsinki;--informed consent, with particular emphasis on the need to inform the patient regarding all aspects of treatment, including any risks or presumed complications that might arise during and after treatment;--professional liability, drawing a distinction between moral responsibility (regulated by the Code of Professional Practice) and legal liability (involving the legal field). Lastly, the authors underline the need to perfect and increase the awareness of the "management" risk of a dental practice, drawing special attention to the need to keep precise records on patients receiving treatment: radiographical and photographical examination, remote and recent medical history, the current state of teeth and gums, the presence of clicks and/or crepitus on ATM, cephalea, spontaneous and/or provoked pain involving masticatory and cervical muscles, obtaining the informed consent of the patient.

Ethics, Dental↗

PubMed1991

[Understanding lateral and vertical gutta-percha condensation technique].

After a brief analysis of the materials and of the various techniques for filling root canals, the authors present the results obtained by a group of 5th year dental students of the Corso di Laurea in Odontoiatria e Protesi Dentaria from their first experiences at filling root canals. The techniques they used, lateral and vertical condensation of gutta-percha, were chosen for their widespread use and for the greater guarantee they offer. The results, which confirmed the expectations, show how lateral condensation is easier to perform by dentists with little experience. The vertical condensation technique, judging from our results, should be used only after a dentist has acquired experience and skill, because in the cases we observed there was a consistent unsatisfactory apical closure.

Gutta-Percha↗

PubMed1990

[Effects of paraformaldehyde steam sterilization on K-files].

Success of endodontic therapy depends upon both operator's skill and quality of the employed instruments. K-files have been examined for they probably are the most commonly used endodontic instruments. It is particularly important to take into consideration, together with the original quality of the instruments, its possible changes due to the wear from sterilization. In this paper have been therefore evaluated the characteristics of brand-new K-files from three different manufacturers together with the wear of the same instruments after paraformaldehyde steam sterilization.

Corrosion↗

PubMed1985

Isotachophoresis as a useful tool for monitoring neurological complications of acute leukaemia in children.

Cerebrospinal fluid proteins from 42 children with acute lymphoblastic leukaemia were analysed by isotachophoresis. The isotachopherograms of cerebrospinal fluid taken from patients undergoing central nervous system prophylaxis with neurological complications showed an increase of several peaks (albumin, prealbumin, and an unidentified peak), and changes in the globulin zone, compared with those from patients who had completed central nervous system prophylaxis for at least six months. The most striking finding was that these alterations were not associated with any other biochemical changes in the cerebrospinal fluid, as assayed by routine analysis. Isotachophoresis may be useful in the monitoring of therapy in children affected with acute lymphoblastic leukaemia.

Brain Diseases↗

PubMed1983

Opsonized-zymosan induces a respiratory burst in human blood platelets.

Opsonized-zymosan-stimulated polymorphonuclear cells show a cyanide-insensitive oxygen consumption. We have investigated whether opsonized-zymosan could induce similar metabolic change in human blood platelets. Preparation of intact human blood platelets, obtained by separation through a Ficoll layer (23% w/v) were challenged with opsonized-zymosan. The polymorphonuclear cell contamination was less than 1/10(8) platelets. The opsonized-zymosan-stimulated platelets showed an increase of oxygen consumption. The mean of oxygen burst measured by a polarographic method with a Clark electrode was 11 nmole/10(9) platelets/min (S.E.M. 4; n = 15). The duration of the burst was 2 min. Unstimulated platelets did not show the oxygen burst. The inhibitors of respiratory chain and prostaglandin synthesis completely abolished the oxygen consumption by opsonized-zymosan-stimulated platelets. The simultaneous addition of NADH (1 mM) and opsonized-zymosan induced a burst of oxygen consumption, which occurred after a variable lag phase (10-12 min) from the stimulation, also in the presence of inhibitors. This burst, which lasted about 1 min, amounted to 10 nmole/10(9) platelets/min (S.E.M. 2; n = 15) and it was higher in the presence of NAN3, a catalase inhibitor. Zymosan treated with hydrazine or heated plasma (56 degrees C) did not cause increased oxygen consumption. Inulin or inulin-treated serum did not stimulate platelets. In these experimental conditions some NADH disappeared, as shown by isotachophoresis. The results demonstrated that an immunological stimulus may activate a membrane-linked cyanide-insensitive oxygen metabolizing system.

Blood Platelets↗

PubMed1982

Superoxide-dependent chemotactic activity for PMNs derived from opsonized zymosan-stimulated human platelets.

Previous studies have shown that platelets exhibit a H2O2 producing, NADH-dependent system that is activated by interaction with particulate material. Current evidence suggests that this system could be critically involved in th generation of chemotactic factor(s). In the present studies, chemotactic activity for polymorphonuclear leukocytes of supernatants derived from zymosan-stimulated human platelets has been evaluated using an agarose gel technique. Supernatants of opsonized zymosan-stimulated platelets showed significant chemotactic activity (migration index = 300 +/- 50), in comparison with supernatants prepared from platelet suspensions stimulated with nonopsonized-zymosan (migration index = 10 +/- 15) or resting platelet supernatants (migration index = 15 +/- 15). Furthermore, a marked increase in chemotactic activity of the opsonized zymosan-treated platelet supernatants was demonstrated after the addition of NADH (migration index = 525 +/- 100). The inclusion of specific inhibitors of the cycloxygenase and lipoxygenase pathways resulted in a marked reduction of chemotactic activity, which was restored in the presence of NADH. Further, the addition of superoxide dismutase completely abolished the chemotactic response induced by NADH. These data suggest that platelets are the source of chemotactic factor(s) derived from the activation of a superoxide generating system.

Blood Platelets↗