Biomedical subjects
C Delage
Publications and source records attributed to C Delage.
Malakoplakia of the colon associated with villous adenoma: report of a case.
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[Digestive and cutaneous colonization of Staphylococcus aureus, Pseudomonas aeruginosa and Corynebacterium xerosis in germ-free hairless mice].
Regardless of the method of germ-free HRS mouse contamination, the kinetics of Staphylococcus aureus and Pseudomonas aeruginosa cutaneous colonization was parallel to the kinetics of intestinal colonization; Corynebacterium xerosis, however, was unable to colonize the skin or the gastrointestinal tract. There was little variability in intestinal and cutaneous colonization; this was quite different from observations in man and hairless holoxenic mice. It is possible to use these experimental models to standardize the measurement of antiseptic bactericidal activity in vivo.
[Determination of cadmium by atomic absorption spectrophotometry in view of toxicologic considerations: analysis in food and tobacco].
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Lipiodol ultra-fluid: an antitumor agent-in vitro study.
Lipiodol Ultra-Fluid (LUF), a contrast medium for the diagnosis and treatment of hepatocellular carcinoma (HCC), is used by hepatic intra-arterial infusion. Hepatoma cells are capable of active uptake of LUF and retaining it for prolonged periods of time. These characteristics have important therapeutic potential for the targeting of anticancer drugs and have lead us to form an intratumoral diffuser. But, before in vivo studies, we have investigated in vitro LUF effects on various cell species in order to explain and refine the lipiodol chemotherapy. The antiproliferative and cytotoxic effects of LUF on HepG2 (human hepatoma cells), MCF-7 (human breast cancer cells) murine macrophages and human hepatocytes, were assessed by Trypan blue exclusion and lactico-dehydrogenase extracellular tests. We demonstrated the dose and time-dependent antiproliferative and cytotoxic activities of LUF on cells. Cytotoxicity was different according to cells species, whether or not they had cancerous characteristics and phagocytosis function: this cytotoxicity was very significant on macrophages and was greater for cancerous cells than for human hepatocytes in primary culture. We showed in vitro, for the first time, that LUF was an antiproliferative and cytotoxic agent, because of its active uptake and selective retention which lead to cellular death due to necrosis by lipoperoxidation increase.
[Ultrastructure of a sacral chordoma].
The ultrastructural findings in a sacrococcygeal chordoma are presented. The tumor cells showed various types of vacuoles. The presence of two types of membrane-bound dense bodies and an intracellular fibrillar material were other striking features of the tumor. Most of the present findings are in agreement with other studies and suggest a double differentiation of the tumor cells, epithelial and mesenchymatous. It is believed that the extracellular substance forming the tumor matrix is produced by mature tumor cells. Despite the pleomorphism and the variation of cell components, it is suggested that the stellate, intermediate and physaliferous cells, constitute the same cell at different stages of maturation.
[Determination of lead by atomic absorption spectrophotometry with regard to toxicology research].
The toxicity of lead in the human environment is acquiring a growing interest which lead us to choose a quick, precise, sensitive and specific method able to perform number of measurements. Thanks to the survey consisting in a dosage of lead by atomic-absorption spectrometric, we are able to confirm the advantages and superiority of such a method and to employ it for the toxicologic research of lead in food, water, industrial atmosphere, and biological liquids.
[Primary mesenchymoma of the liver. Ultrastructural study].
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[Tumors of the appendix associating histologic features of carcinoid and adenocarcinoma].
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[Digestive and cutaneous colonization of Staphylococcus epidermidis in hairless axenic mice].
In order to determine the cutaneous and digestive colonization of S. epidermidis in germ-free HRS mice, several groups of animals were contaminated with this strain according to different methods. The bacterial cutaneous enumerations were performed after preliminary crushing of the cutaneous biopsy. In this study, whatever the method of contamination of germ-free HRS mice, the kinetics of S. epidermidis cutaneous colonization was parallel to the kinetics of intestinal colonization. The isolation of bacteria on skin was possible only when microorganisms were eliminated in faeces (6 h after contamination). During a single experiment, there was only slight variability in colonization, whether intestinal or cutaneous. Some differences could be found from one experiment to another, but all animals in the same trial showed the same cutaneous bacterial count. This experimental model is characterized by an interrelation between the intestinal and cutaneous ecosystems.
MCF-7 cell cycle arrested at G1 through ursolic acid, and increased reduction of tetrazolium salts.
The effect of ursolic acid on the proliferation of MCF-7 human breast tumor cells was studied. During investigations of the anti-proliferative effects of this triterpene, we observed a clear difference between MTT colorimetric assay and direct cell counting, particularly 24 h after drug treatment. The MTT assay showed a stimulation of formazan production in the first 24 h exposure of cells to drug. The maximum stimulation was obtained with 15 and 20 microM of ursolic acid (about 30 - 40% of increase with respect to control); however, the number of cells was not increased as revealed by direct cell counting. Ursolic acid is a potent inhibitor of MCF-7 cell proliferation. This triterpene exhibits both cytostatic and cytotoxic activity. It exerts an early cytostatic effect at G1 followed by cell death. Cell cycle analysis is performed by propidium iodide staining and flow cytometry technique. These results suggest that alterations in cell cycle phase redistribution of MCF-7 human breast cancer, by ursolic acid, may significantly influence MTT reduction to formazan.