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C Diaconu

Publications and source records attributed to C Diaconu.

At least 19 recordsLinked to original sources
PubMed1996

Spectral and interferometrical study of the interaction of haemin with glutathione.

The interaction of haemin with reduced glutathione (GSH) was investigated in vitro and the association constant was determined by both spectrophotometric and interferometric methods. In order to elucidate the nature of this interaction, the reaction of haemin with a typical reductive agent (sodium dithionite) and a typical chelating agent for hem-iron (histidine) was also studied. Haemin-induced haemolysis in the presence and absence of GSH was monitored spectrophotometrically. The results outline the role of GSH at physiological concentration in preventing erythrocyte damage by haemin.

Erythrocyte Membrane↗
PubMedDate not supplied

Detection of human papillomavirus gene sequences in cell lines derived from laryngeal tumors.

The role of Human Papillomaviruses (HPV) in laryngeal carcinomas has been studied with conflicting results. To evaluate the etiologic relationship between HPV infection and epithelial malignancy of the larynx we studied five laryngeal carcinoma cell lines obtained from patients undergoing surgery for laryngeal tumors. The paraffin embedded biopsy samples of the original tumor and different passages of the new established cell lines were investigated by PCR with consensus primers specific for HPV DNA. The findings indicate that HPV infection is associated with some larynx carcinomas. The positive association has been enhanced when a method of enrichment of epithelial cells from fresh tumor samples was used. All tumor cells enriched smears were positive for HPV DNA not only by PCR but also by in situ hybridization (ISH). Investigated by PCR, different passages of larynx tumor cell lines maintained expression of HPV DNA. At subsequent passages ISH gives constantly no signals suggesting a minimal amount of viral harbored sequences. In one cell line propagated more than 60 population doublings, the chromosomal frequency distribution shifted from modal number 46 at the 5(th) passage to 63 at the 60(th) passage. The mechanisms by which persistent HPV infection maintains continuous cell proliferation were discussed.

Aged↗
PubMedDate not supplied

"Trial moves rapidly on, when the judge has determined the sentence beforehand" (W. Scott: Ivanhoe, 36) or pitfalls in serosurvey of anti-hepatitis C antibody in children.

Hepatitis C is and will be a major public health concern. Confirmed infections were reported from all Romanian counties but important differences between regions raise several explanations. Differences may reflect the different levels of testing, the performances of laboratories in confirming initially reactive samples or the risk factors higher prevalence. We have suggested that the prevalence of anti HCV infections can be a surrogate marker for the quality of parenteral medical or paramedical interventions. Present report identified additional problems in the surveillance of HCV infection in children. We screened 1787 samples from children hosted in orphanages (children under three years old) or in preschool children institutions (between 3-7 years old). We detected 31 repeatedly reactive samples with two EIA screening kits but confirmed only 8 in WB anti HCV. Four confirmed samples come from children under four months old suggesting maternally transmitted antibodies. In highly endemic area, many infants have maternally derived antibodies and the wane of reactivity comes with age above 12 months. Therefore, the prevalence of anti HCV antibody in infants reflects the prevalence in adult population. Confirmatory tests are mandatory for the serosurvey in children. More frequent than adults samples, children EIA reactive samples give indeterminate or negative Western Blot profiles. Only the viral load evaluation can confirm those samples as false positive or, on the contrary, samples at the beginning of seroconversion.

Child↗
PubMedDate not supplied

Low rates of seroconversion after hepatitis B vaccination in orphanges with high prevalence of virus carriers.

A serosurvey of Hepatitis B infection markers was conducted in two orphanages that adhered to Hepatitis B vaccination policy. In spite of comparable sizes (80-90 children per facility), housing conditions and infection control practices, the level of HbsAg endemicity was different in each unit in direct relation with the mean age of the children. The prevalence of HbsAg carriers and the interval spent in collectivity strongly affect the seroconversion rate after HB vaccination. Other elements that can explain the low seroconversion rate were: the proportion on fully vaccinated children, the number of vaccine administered doses and the delayed age at which childhood immunization schedule was initiated. In order to increase the protective antibody response, booster doses were administered to a limited number of nonseroconvertors or to children with a nonprotective level of anti-HBs antibody (< 10 UI). This intervention provides evidence of prompt rising in antibody titers, comparable with titers found in children with wild infection.

Carrier State↗
PubMedDate not supplied

[The segmentary colectomy or the left hemicolectomy for sigmoid cancer].

UNLABELLED: The aim of this study was to show the benefits of segmentary colectomy (Raybard) for tumors located on sigmoid colon. A retrospective study was carried on 90 observations performed in the III-rd Surgery Clinic, Iaşi between 1994 and 1998. Types of surgery performed were: left hemicolectomy (7 observation); segmentary colectomy (54 observation); left lower colectomy (17 observation); Hartmann operation (12 observation). CONCLUSIONS: This analysis showed the same survival for segmentary colectomy and left hemicolectomy. We consider segmentary colectomy the basic operation for this type of cancer.

Carcinoma↗
PubMedDate not supplied

[Could biological aggressivity markers of breast cancer alter the therapeutic course? Preliminary results].

The aim of this study is to justify an individual therapeutical attitude in breast cancer, related to diversity of breast tumors, aggressiveness grade and metastatic potential. Between January 2000--December 2001, 150 patients were admitted with breast cancer (stage II and III) and underwent surgery in our department. We selected 75 cases in our study. In 51 (68%) cases the first therapeutical method was surgery, in 15 (20%) cases surgery was performed after chemotherapy, in 2 (2.66%) cases after radiotherapy and after chemotherapy and radiotherapy in 7 (9.33%) cases. We evaluated several classical factors and new immunohistochemical markers with an important value for diagnosis, prognosis and therapy: oestrogen and progesterone receptors, c-erb B2, pS2 and p53 proteins, von Willebrand factor. Several factors had a predictive role regarding the response to chemotherapy. These predictive factors will improve the histopathological diagnosis. The oncoproteins and hormonal receptors also will evaluate with more accuracy the metastatic risk and will assure a better therapy decision.

Biomarkers, Tumor↗
PubMedDate not supplied

[Surgical initial attitude in epithelial ovarian advanced cancer].

UNLABELLED: The purpose of this work is to show the value of aggressive excisional surgery in epithelial ovarian advanced cancer (COEA). MATERIAL AND METHOD: Between May 1993-May 1997, there were 103 obs. with ovarian diseases; 28 obs. (27.1%) with COEA and 75 obs. (72.9%) with benign diseases. All the patients had a subtotal hysterectomy with bilateral anexectomy and omentectomy. The operation was extended to other anatomic elements; in the case of 4 obs., we practiced a segmentary resection of left colon, 2 obs. had an anterior resection of the rectum and a partial resection of the pelvic peritoneum, 15 obs. suffered an excision of tumoral masses from the gastro colic omentum and the right parietocolic space and 7 obs. had a resection of pelvic peritoneum and of the right parietocolic space peritoneum. The optimal cytoreduction with a tumoral residuum less than 2 cm was realized for 23 obs. (82.14%) and for 5 obs., the tumoral residuum was greater than 2 cm (17.76%). The medium survival of the patients with a tumoral residuum inferior to 2 cm was 41 months, at 4 years 54% of the observations being alive, compared to only 14 months for the patients with tumoral residuum superior to 2 cm, at 4 years the survival being only of 15%. All the observations have beneficiated of chemotherapy. The disease reappeared for 23 obs. after a variable period of time. It's treatment consisted of: surgery followed by chemotherapy for 18 obs. and chemotherapy of second line alone for 5 obs. CONCLUSIONS: The ovarian epithelial cancers, famous for an intermediary answer to chemotherapy, lead to an aggressive surgical management with partial extra genital organ sacrifices, to offer a chance of life.

Aged↗
PubMedDate not supplied

[Synchronous colonic cancers].

UNLABELLED: The aim of this study is to present the difficulty of an accurate preoperative diagnostic for synchronous colonic cancers and to sustain the necessity of total colectomy. A retrospective study was carried out on 16 patients hospitalized in the IIIrd Surgical Unit, St. Spiridon Hospital, U.M.Ph. "Gr.T.Popa" Iasi between 1990-1999. The surgical procedures were: extensive colectomy with ileo-sigmoid anastomosis, segmentary colectomy, total colectomy with ileo-rectal anastomosis. RESULTS: Perioperative mortality: zero; uneventfully recovery for all patients. A metachronous lesion 3 years after the first operation was detected in one patient; postoperatively, one patient developed occlussion 3 months after, requiring re-operation. CONCLUSIONS: 1. synchronous colonic cancers are closely related with a genetic instability of the colonic mucosa; 2. total colectomy is a safe manner to prevent metachronous lesions.

Adenocarcinoma↗
PubMedDate not supplied

[Surgical treatment of locally advanced esophago-gastric cancer; preliminary results].

UNLABELLED: The aim of this paper is to sustain the palliative resection in neoplasm of the esophago-gastric junction, as a surgical approach that allows a better post-operative life comfort in comparison with simple gastrostomy. 62 observations with proximal neoplasm of the stomach (12.5%) were identified between January 1996-August 2001, representing 12.5% of the 496 patients with gastric neoplasm admitted in our unit in the same period. Out of these 62 cases, 55 (88.71%) underwent surgical procedures. Our attitude was aggressive in 25 cases. 40.32%, including the locally advanced lesions with palliative surgical indications (18 obs.). The other 30 patients underwent: 10 laparotomies, 5 gastrostomies and 15 jejunostomies. Local invasion to the neighboring organs imposed partial resection of the transverse colon--1 obs., of the transverse mesocolon--2 obs., and corporeo-caudal pancreatectomies--3 obs. The surgical approach was a left abdomino-thoracic incision, with total gastrectomy and distal esophagectomy, with N1 and N2 lymphadenectomy, splenectomy, and esojejunal intrathoracic anastomosis, with a Roux-en-Y loop, with or without jejunostomy (13 obs.). The immediate post-operative complications were 8 anastomotic leakage, one duodenal stump fistula, one occlusion due to a jejunostoma, and 13 extradigestive complications. There were 5 post-operative deaths. CONCLUSION: Neoplasm of the esophago-gastric junction is lately diagnosed, but whenever is possible, total gastrectomy with distal esophagectomy should be carried out.

Adenocarcinoma↗
PubMedDate not supplied

[The diagnosis and treatment of benign tumors of the subdiaphragmatic digestive tract].

One hundred twenty-two benign tumors of subdiaphragmatic digestive tract admitted in the interval 1975-1988 at the III-rd Surgical Clinic of Iaşi are reported. Out of these patients 120 required surgical treatment, the remainder of 2 being treated conservatively (diffuse intestinal angiomatosis, Peutz-Jeghers' syndrome). The clinical evolution being atypical, the surgical intervention was required, in most of the cases, due to hemorrhagic and occlusive complications. Histologically, the polyps and schwannomas were prevalent. The diagnostic difficulties, especially in the cases with jejuno-ileal localization, are mentioned.

Diaphragm↗
PubMedDate not supplied

[Cancer of the upper gastric pole--possibilities and limitations].

An analysis of the cases encountered over the last 5 years revealed a high frequency of gastric cancers located in the upper pole area of the stomach. These represented 23% of all gastric cancers. The low number of cases with this localization that could benefit from gastric resection (38,7%), in contrast with distal locations (65,4%) suggest either late detection or high invading potential of these cancers. Oeso-gastric resection - the only efficient curative or palliative treatment - was adapted according to site and extension of the tumour in both organs. In 6 cases partial resection of the thoracic oesophagus was necessary, and in another 4 cases total gastrectomy was performed. In 8 patients upper polar resection - including the oesophagus - was satisfactory from the oncologic viewpoint, and was well-tolerated by the patients.

Adult↗