Biomedical subjects

C Doser

Publications and source records attributed to C Doser.

3 recordsLinked to original sources

PubMed1991

Fishhook injuries: a prospective evaluation.

A prospective study was conducted involving 100 nonrandomized, consecutive patients who suffered fishhook injury during the summer of 1990 in Alaska. Time of injury prior to admission to the emergency department, location of fishhook, method of removal, wound care, systemic antibiotic prophylaxis, anesthetic, tetanus immunization status, fishhook size, and complication rate were evaluated. Results of this study suggest that most fishhook injuries involve the hands or head and that postremoval wound care including oral antibiotic therapy may not be critical. Local anesthetics and simple removal techniques are adequate for nearly all fishhook injuries. Routine systemic antibiotic prophylaxis is not necessary for uncomplicated soft tissue injury due to fishhooks not involving cartilage or tendons.

Alaska↗

PubMed1989

Influence of carbohydrates on the cytotoxicity of an aqueous mistletoe drug and of purified mistletoe lectins tested on human T-leukemia cells.

Partially and highly purified lectins from Viscum album L. (mistletoe) cause a dose-dependent decrease of viability of human leukemia cell cultures, MOLT-4, after 72 h treatment. The LC50 of the partially purified lectin was 27.8 ng/ml, of the highly purified lectin 1.3 ng/ml. Compared to the highly purified lectin a 140-fold higher protein concentration of an aqueous mistletoe drug was required to obtain similar cytotoxic effects on MOLT-4 cells. Cytotoxicity of the highly purified lectin was preferentially inhibited by D-galactose and lactose, cytotoxicity of the mistletoe drug and the partially purified lectin were preferentially inhibited by lactose and N-acetyl-D-galactosamine (GalNAc). Two lectin fractions with almost the same cytotoxic activity on MOLT-4 cells but with different carbohydrate affinities were isolated by affinity chromatography from the mistletoe drug: mistletoe lectin I with an affinity to D-galactose and GalNAc and mistletoe lectin II with an affinity to GalNAc. The lectin fractions and the mistletoe drug inhibited protein synthesis of MOLT-4 cells stronger than DNA synthesis. Furthermore a subpopulation of MOLT-4, resistant to cytotoxic doses of both the mistletoe drug and the mistletoe lectins, was shown to exhibit a reduced amount of GalNAc and N-acetyl-D-glucosamine in their cellular glycoproteins which are probably responsible for the binding of the cytotoxic lectins. These results indicate that lectins are the main toxins in the mistletoe drug.

Carbohydrates↗

PubMed1986

Differences in the in vitro effectiveness of preparations produced from mistletoes of various host trees.

The in vitro effectiveness of three Helixor preparations produced from mistletoes of different host trees on suspension cell cultures of the human leukemia cell line Molt 4 has been compared by means of dose-response investigations. After 72 h treatment the preparation produced from mistletoes of the appletree (Malus) shows the strongest effect on the growth and viability of the cell cultures. The preparation produced from mistletoes of the fir (Abies) shows also an evident but much weaker effect, whereas the preparation produced from mistletoes of the pine (Pinus) causes a weak effect only at a very high dosage. As shown in 24-h incorporation experiments with 3H-labelled DNA-, RNA- and protein precursors the preparation produced from mistletoes of the apple-tree reduces the protein synthesis of the cells to a greater extent than the DNA-and RNA-synthesis.

Cell Line↗