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Biomedical subjects

C Duret

Publications and source records attributed to C Duret.

6 recordsLinked to original sources
PubMed1992

Protection of mice and swine from pseudorabies virus conferred by vaccinia virus-based recombinants.

Glycoproteins gp50, gII, and gIII of pseudorabies virus (PRV) were expressed either individually or in combination by vaccinia virus recombinants. In vitro analysis by immunoprecipitation and immunofluorescence demonstrated the expression of a gII protein of approximately 120 kDa that was proteolytically processed to the gIIb (67- to 74-kDa) and gIIc (58-kDa) mature protein species similar to those observed in PRV-infected cells. Additionally, the proper expression of the 90-kDa gIII and 50-kDa gp50 was observed. All three of these PRV-derived glycoproteins were detectable on the surface of vaccinia virus-PRV recombinant-infected cells. In vivo, mice were protected against a virulent PRV challenge after immunization with the PRV glycoprotein-expressing vaccinia virus recombinants. The coexpression of gII and gIII by a single vaccinia virus recombinant resulted in a significantly reduced vaccination dose required to protect mice against PRV challenge. Inoculation of piglets with the various vaccinia virus-PRV glycoprotein recombinants also resulted in protection against virulent PRV challenge as measured by weight gain. The simultaneous expression of gII and gp50 in swine resulted in a significantly enhanced level of protection as evaluated by weight evolution following challenge with live PRV.

Animals↗
PubMed1986

[Diagnosis of enzootic bovine leukosis by the ELISA test of mixed and individual milk].

The ELISA test was applied to mixed milk from 325 cowsherds of the Landes region of France. From the 88 cowsheds giving a positive or inconclusive response, individual samples of milk were studied by the ELISA test, and individual samples of serum by the agar immunodiffusion test (1734 cows). The same procedure was carried out on 1250 animals in a sample of 49 cowsheds chosen at random from amongst the 237 cowsheds whose mixed milk gave a negative response to the ELISA test. The results confirmed the importance of the ELISA test applied to mixed milks, provided that the samples are studied several times per year, so as to minimize default errors. The study of individual milk allowed the identification of 92 to 94% of the infected animals, with a specificity of 98 to 99%. The systematic and repeated testing of mixed milks by the ELISA test should allow initial detection of infection and supervision of a satisfactory cost-efficiency report in the decontaminated cowsheds.

Animals↗
PubMed1985

Freeze-dried vaccine against Rinderpest: stability and activity study.

A freeze-dried vaccine against Rinderpest was prepared from modified virus multiplied in calf kidney cell culture. Characteristics of the vaccine are as follows: high titre after freeze-drying (10(4) CCID50/dose), well-adapted freeze-drying stabilizer which ensures maintenance of the infective titre of the vaccinal virus, even under severe conditions (3.5 days at +45 degrees C), use of an appropriate solvent: magnesium sulphate molar solution or more simply physiological saline (for stability after reconstitution even at high temperatures--up to 4 h at +45 degrees C). The activity of the vaccine, tested in cattle by antibody titration and resistance to specific challenge perfectly satisfies requirements set by the WHO and OIE.

Animals↗