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C E CLIFTON

Publications and source records attributed to C E CLIFTON.

At least 19 recordsLinked to original sources
PubMed1963

OXIDATIVE ASSIMILATION BY BACILLUS MEGATERIUM.

Clifton, C. E. (Stanford University, Stanford, Calif.). Oxidative assimilation by Bacillus megaterium. J. Bacteriol. 85:1365-1370. 1963.-Washed suspensions of Bacillus megaterium oxidized to CO(2) about 39% of the U-C(14)-glucose supplied and incorporated about 37% of the label by the time a marked break in the rate of O(2) consumption was noted. Almost one-half of the label was lost from the cells on acidification of the suspension. The remainder of the C(14) was present in the supernatant fluid, primarily in forms as yet unidentified, but other than carbohydrate. Both the Embden-Meyerhof and hexose monophosphate pathways of oxidation were involved. Endogenous respiration appeared to be inhibited only to a slight extent in the presence of an exogenous substrate. C(14) appeared in all fractions of the cells; the highest percentage of firmly bound C(14) was present in hot 5% trichloroacetic acid-insoluble matter. A decrease in C(14) content of the various fractions was noted during endogenous respiration of cells labeled during growth. Pyruvate and acetate were oxidized very slowly by B. megaterium. The results indicate the complexity of oxidative assimilation and the dynamic state of cellular metabolism.

Acetates↗
PubMed1963

INFLUENCE OF GROWTH MEDIUM ON ASSIMILATORY ACTIVITIES OF ESCHERICHIA COLI.

Clifton, C. E. (Stanford University, Stanford, Calif.). Influence of growth medium on assimilatory activities of Escherichia coli. J. Bacteriol. 85:1371-1377. 1963.-Nutrient agargrown cells assimilate about 50% of the glucose added to washed suspensions (phosphate buffer, pH 7.2, 30 C), as indicated by O(2) consumption or C(14)-uptake from uniformly labeled glucose. Most of the nonassimilated glucose is oxidized, but a small portion remains in forms other than glucose in the suspension medium. Cells from glucose agar, however, assimilate only about 20 to 30% of the U-glucose and ferment a considerable portion of the remainder, the extent of each being determined in part at least by growth conditions. Evidence is presented that this behavior is the result of metabolic controls induced during growth. C(14) from U-glucose appeared in all fractions of either agar- or glucose agar-grown cells, the highest percentage being present in cellular matter insoluble in hot 5% trichloroacetic acid. Data on the distribution of C(14) from 1-, 2-, and 6-glucose, and from acetate and pyruvate, are also presented.

Acetates↗
PubMed1962

Oxidative assimilation and distribution of glucose in Bacillus cereus.

Clifton, C. E. (Standford University School of Medicine, Stanford, Calif.). Oxidative assimilation and distribution of glucose in Bacillus cereus. J. Bacteriol. 83:66-69. 1962-Exogenous C(14)-labeled glucose is taken up rapidly by washed cells of Bacillus cereus and apparently enters a metabolic pool soluble in cold 5% trichloroacetic acid. From this pool the label passes rapidly into other cellular fractions, primarily materials soluble and insoluble in hot 5% trichloroacetic acid, which may constitute endogenous reserves of the cells. There is a marked lag in the liberation of C(14)-labeled CO(2); little or no lag is observed in the slightly enhanced rate of oxygen consumption noted in the presence of exogenous glucose. Assimilation, in part at least, appears to be at the expense of cellular reserves which appear to be replenished or replaced by the assimilated substrate.

Bacillus↗
PubMed1961

Endogenous respiration of Bacillus cereus.

Clifton, C. E. (Stanford University, Stanford, Calif.), and J. M. Sobek. Endogenous respiration of Bacillus cereus. J. Bacteriol. 82:252-256. 1961.-The endogenous respiration of washed cells of Bacillus cereus varies with the nature of the growth medium and with time. The respiratory quotient of cells harvested from nutrient agar remained quite constant around 1.00 over a 2-hr period of respiration, whereas that of cells grown on glucose-nutrient agar decreased from 0.97 for the first hour to 0.87 for the second hour. Considerable amounts of ammonia were formed, the number of moles per mole of oxygen consumed decreasing with time for agar-grown cells and increasing for glucose-grown ones.C(14)-labeled, agar-grown cells utilized materials insoluble in cold or hot 5% trichloroacetic acid, ethanol, or chloroform as their endogenous substrate, the same behavior being noted with glucose-grown cells except that they utilized both hot trichloroacetic-soluble and -insoluble materials. These results indicate that the bulk of the endogenous substrates are chemically complex and, at least in part, are nitrogenous in character.

Agar↗