Biomedical subjects

C E Shideler

Publications and source records attributed to C E Shideler.

6 recordsLinked to original sources

PubMed1984

Lithium stimulation of murine hematopoiesis in liquid culture: an effect mediated by marrow stromal cells.

Lithium has previously been observed to stimulate in vitro Dexter culture hemopoiesis with increases in granulocytes, megakaryocytes, and pluripotent stem cells (CFU-S). In the present study, a two-phase murine Dexter culture system was established to study the mechanism of lithium-mediated stem cell stimulation. Different lots of horse sera or fetal calf sera were found to have markedly different effects on Dexter culture growth; given the appropriate sera supplementation, supernatant cells from Dexter cultures established from C57BL/6J mice 3 wk previously were free of stromal-forming capacity, but had stem cells and could grow on 900-950 R irradiated stroma. Conversely, in vitro irradiation (900-950 R) of 3-wk cultures resulted in a stem-cell-free adherent monolayer that could support growth for up to 9 wk in culture. The stroma from Dexter cultures preexposed to lithium chloride (1.0 mmole/liter) for 3 wk, irradiated (900 R), and then refed with 3-wk Dexter supernatant cells has an enhanced capacity to support cell production, CFU-S, and probably granulocyte-macrophage colony-forming cell (GM-CFU-C) production, as compared to stroma not preexposed to lithium. Lithium carryover was ruled out in these experiments. These data indicate that lithium stimulates CFU-S and in vitro granulopoiesis by an indirect effect on a radioresistant adherent stromal cell.

Animals↗

PubMed1983

Vitamin B6: an overview.

A wealth of information has accumulated over the past several decades with respect to the B-complex vitamins and their roles in health and disease. This paper focuses on a member of the B complex, vitamin B6, reviewing its biochemical functions, associated disease states, and laboratory evaluation.

Adult↗

PubMed1981

Erythrocyte polyamine determinations in patients with head and neck cancer.

Erythrocyte polyamine levels were measured in the blood from 29 untreated patients whose conditions were diagnosed as head and neck cancer. Only nine (31%) of these patients had elevations of erythrocyte spermidine and/or spermine levels above the reference ranges determined for normal persons. However, a positive correlation was observed between the erythrocyte spermidine levels and the clinical tumor stage. Serial erythrocyte polyamine determinations were performed on the blood from 12 of these patients before and after either surgical or radiation therapy. In 11 of the cases, the erythrocyte spermidine levels decreased after tumor therapy regardless of whether there was prior elevation above the reference range. The erythrocyte spermine levels in these patients were more variable in their response to tumor treatment. Therefore, although erythrocyte polyamine levels were only slightly to moderately elevated in response to the small tumors characteristic of the head and neck, the measurement of erythrocyte spermidine, potentially, may offer a simple and effective means of monitoring the course of therapy used in patients with head and neck cancer.

Carcinoma, Squamous Cell↗

PubMed1981

Use of a flow-injection sample manipulator as an interface between a "high-performance" liquid chromatograph and an atomic absorption spectrophotometer.

We describe an integrated, molecular-absorbance, atomic absorption instrument for studying metal/ligand binding in clinical samples. For an interface between the "high-performance" liquid chromatograph and the atomic absorption instrument we used a flow-injection sample manipulator, thus allowing both the chromatograph and the atomic absorption detector to operate at their separate optimum conditions. After specimen separation with a gel permeation column, we measured the molecular components of the column eluate by molecular absorbance spectrometry and the atomic components (calcium and magnesium) by flame atomic absorption spectrophotometry. This instrument system is capable of separating and analyzing multiple components within 20 min of injection of the sample on the column. The chromatograms presented demonstrate the utility of the system for investigating metal binding to a variety of ligands in clinical samples.

Calcium↗

PubMed1980

Automated multiple flow-injection analysis in clinical chemistry: determination of total protein with Biuret reagent.

We have examined the feasibility of the automated multiple flow-injection technique for application to clinical chemistry by adapting to this system the biuret method for the determination of total protein. Samples were discretely and rapidly introduced into a continuously flowing, nonsegmented reagent stream by means of an automatic sampler and high-pressure injection valve. Pumps operating at 1380-2070 kPa (200-300 psi) were utilized to introduce the biuret reagent and saline diluent into the system separately at flow rates of 72 and 47 microL/s, respectively. Use of 20-microL sample and a 3.0-s reaction-delay coil was adequately sensitive for analysis for total protein by this method. Samples were analyzed at a rate of 150/h with no detectable between-sample carryover. Within-run precision studies yielded relative standard deviations of 2.5% and less. Total protein values obtained by this method correlated well with those obtained by centrifugal analyzer and bubble-segmented continuous-flow biuret methods.

Autoanalysis↗

PubMedDate not supplied

Effects of naloxone and neuroleptic drugs on muscle rigidity and heart rate of the nervous Pointer dog.

Muscle rigidity, a persistent physiological characteristic of the nervous Pointer dog, is not only attenuated by the major neuroleptic drugs, haloperidol and pimozide but also by naloxone. In addition, naloxone administration results in a modest but significant increase in heart rate of these subjects. The data obtained in this study add support for the concept that an abnormality in central nervous system dopaminergic function is involved in the genesis of abnormal behavior of this animal model.

Animals↗