South east Peru II: Delivery of primary medical care by hovercraft along the Apurimac river.
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Biomedical subjects
Publications and source records attributed to C E White.
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Twenty-two Hampshire-Yorkshire X Large White sows of second and third parity were allotted randomly to one of three dietary treatments. Five sows were fed 6.0 kg/day of a corn-soybean meal lactation diet (control diet). Twelve sows were fed the control diet in which 24% of the composition was supplied by corn syrup containing 72% fructose on a dry matter basis (fructose diet) and five sows were fed the control diet in which 24% of the composition was supplied by powdered dextrose (glucose diet). All diets were fed from days 1 through 21 of lactation. Blood was collected from all sows immediately prior to feeding and hourly for 6 hours postprandial via jugular vein cannulae following a single feeding on seven separate but nonconsecutive days during the 21-day period. Fructose was absorbed from the digestive tract of sows as evidenced by elevated (P less than 0.01) conventions of fructose in plasma. Sows fed the fructose diet also had higher (P less than 0.01) plasma glucose concentrations than did those fed the glucose and control diets. The concomitant elevated glucose concentration following ingestion of the fructose diet was not associated (P greater than 0.10) with increased insulin concentration. Fructose in plasma was associated with a slight but significant increase in insulin although the mean concentration of insulin in plasma was only one-third that measured in sows fed the glucose and control diets. These data suggest that fructose in vivo has a glucose-sparing effect presumably mediated through a physiological mechanism that lowers insulin concentration.
Yield and composition of milk and growth of nursing pigs in response to dietary treatment were estimated from 25 lactating sows during a 22-d period. Eight sows were fed 6 kg/d of a corn-soybean control diet (C sows). Nine were fed the control diet in which approximately 6.5 g X kg body weight (BW-1) X d-1 of carbohydrate was supplied by fructose corn syrup (F sows) and eight were fed the control diet containing equivalent carbohydrate supplied by powdered dextrose (D sows). Blood samples collected via jugular cannulae were analyzed for plasma concentrations of fructose, glucose and insulin. Concentrations of fructose and glucose from F sows were significantly higher throughout the study than that from D and C sows, while insulin concentration was approximately 2.5-fold lower. Milk yield from F sows on d 14 and 21 was significantly higher and pigs weaned on d 21 were heavier than those from D and C sows. Sows fed the diet containing fructose experienced significant BW loss during lactation. Coefficients of gross correlation across treatments showed milk yield and litter weight gain to be negatively associated with percentages of protein, lipids and total solids in milk, but positively associated with concentrations of lactose and gross energy. Nursing pig weight gain at weaning was more responsive to total yields of milk and milk nutrients than to composition. These data support the hypothesis that source of metabolizable energy (ME) affects milk yield, composition and efficiency at which the sow converts dietary nutrients into milk.
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Eight fetal pigs, in utero, were injected ip with 20 microCi/fetus [U14C]-fructose between d 55 and 65 pregnancy. The isotope was allowed to equilibrate between blood and tissues within injected fetuses for a period of 240 min. Fetal pigs were then sacrificed and nucleic acids were extracted from cold tissue homogenates of skeletal muscle and liver. Nuclide disintegrations per minute recovered in extracted DNA and RNA were used to calculate incorporation of labeled C from fructose. The recovery of labeled C per mmol of nucleic acids from skeletal muscle was greater (P less than .05) than that from liver. Relative incorporation of labeled C into skeletal muscle RNA (395.9 pmol/mmol) was greater (P less than .05) than for DNA (189.5 pmol/mmol). The same trend was observed for liver RNA (78.0 pmol/mmol) and DNA (55.6 pmol/mmol), but differences were nonsignificant. These data suggest that at least part of the high concentration of endogenous fructose measured in fetal pigs in utero is involved in synthesis of nucleic acids, thereby providing substrate for anabolic functions necessary for fetal growth and development.
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A pedicle skin flap based on the caudal superficial epigastric artery was created in 12 cats. The artery was ligated and the vein left intact. Six cats were treated with hyperbaric oxygen at 2 atm absolute for 90 minutes daily for 14 days, starting the day after surgery. Skin flap color was significantly better in the treated cats on days 1, 2, and 3. The amount of exudate was significantly less in the treated cats on day 3. There was no difference in the amount of swelling between treated and nontreated control cats. Although five of six of the treated cats had 100% skin flap survival, there was no significant difference in total flap survival between treated and nontreated control cats.