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Biomedical subjects

C E Wilde

Publications and source records attributed to C E Wilde.

At least 37 records · Page 2Linked to original sources
PubMed1980

Amniotic fluid alkaline phosphatase, gamma-glutamyltransferase, and 5'-nucleotidase activity from 13 to 40 weeks' gestation, and alkaline phosphatase as an index of fetal lung maturity.

Reference ranges for amniotic fluid alkaline phosphatase, gamma-glutamyltransferase, and 5-nucleotidase are described from 13 to 40 weeks' gestation. Gamma-glutamyltransferase and 5-nucleotidase activities peak early in the second trimester and then decrease to low values. Alkaline phosphatase shows a similar pattern of activity from 13 to 29 weeks' gestation, but thereafter activity increases to term; this late increase is mainly related to the heat-labile particulate form of alkaline phosphatase. Total and heat-labile alkaline phosphatase alone or expressed as a ratio with gamma-glutamyltransferase can be used with or as an alternative to lecithin/sphingomyelin ratios in the investigation of fetal lung maturity. A total alkaline phosphatase activity of 0.36 mukat/L and an alkaline phosphatase/gamma-glutamyltransferase ratio greater than 2 indicate pulmonary maturity.

Alkaline Phosphatase↗
PubMed1978

The incidence and likely origins of serum particulate alkaline phosphatase and lipoprotein-X in liver disease.

Both serum particulate alkaline phosphatase and serum lipoprotein-X have been proposed as diagnostic markers for obstructive liver diseases. In this study their diagnostic efficiencies have been compared with other biochemical indicators of liver function and the relative incidence of these two markers has been determined in 241 patients with well defined liver disease. Although these markers appeared together most frequently in obstructive liver disease they were both present in some patients with liver disorders where obstruction was unlikely. One marker was present, independently of the other in 35% of the patients studied, mainly those without apparent cholestasis. The reasons for this are discussed in terms of the likely origins of the two markers and the probability that serum particulate alkaline phosphatase exists in two forms, as part of a multienzyme plasma membrane complex and as soluble liver enzyme associated with lipoprotein-X.

Alkaline Phosphatase↗
PubMed1978

Polymerization of human immunoglobulin M.

The repolymerization of human IgM following mild reductive cleavage was studied as a model for intracellular polymer assembly. Repolymerization was found to require the presence of J chain and a disulfide exchanging system which could be furnished either intrinsically by the use of the monofunctional thiol mercaptoethylamine or extrinsically by the inclusion of a protein-mercaptan mixed disulfide, and/or a disulfide exchanging enzyme. The degree of repolymerization was dependent on the extent of monomer reduction and the product covalently incorporated one J chain per five monomer units. Disulfide exchanging enzyme probably served as a source of mixed disulfides rather than as an enzymatic catalyst of the reaction. These results are discussed in terms of a tentative mechanism for IgM polymerization.

Humans↗
PubMed1978

Structural studies on protein products of murine chromosome 17. III. Partial amino acid sequence of an H-2Kq molecule.

Twenty-one of the amino terminal twenty-seven amino acid residues have been assigned in the murine H-2Kq molecule and several additional assignments and corrections have been made in both the H-2Kk and H-2Kb molecules. The study shows that 1) H-2Kk and H-Kq, which are serologically related, have an identical amino acid sequence for all twenty positions that can be compared, 2) the H-2Kk and H-2Kq molecules differ from H-2Kb at only two positions--9 and 22, and 3) the additional sequence data on the H-2Kk, -Kb, and -Kq molecules increases the already striking homology between murine and human transplantation antigens.

Amino Acid Sequence↗
PubMed1976

Pituitary function in patients receiving intermittent cytotoxic and corticosteroid therapy for malignant lymphoma.

Diurnal variation in plasma-cortisol was studied immediately before and after intermittent steroid therapy in seven patients receiving monthly courses of quadruple chemotherapy for Hodgkin's or non-Hodgkins lymphoma over a period of 6 months. The serum-thyroid-stimulating-hormone (T.S.H.) response to intravenous T.S.H.-releasing factor was also measured before and during the first course and before the second and fourth courses. The morning plasma-cortisol concentration fell significantly over 6 months when measured immediately before the start of each course. The mean evening cortisol concentration also fell over this period. In most patients the T.S.H. response showed a downward trend during treatment, although in two patients the response returned to normal whilst they were still undergoing therapy.

Adult↗
PubMed1976

Diet and oral antidiabetic drugs and plasma sugar and insulin levels in patients with maturity-onset diabetes mellitus.

A longitudal study was carried out to separate the effects of treatment with diet from those of treatment with glibenclamide and phenformin on the oral glucose tolerance test (OGTT) plasma sugar and insulin levels in 118 patients with maturity-onset diabetes mellitus. Both drugs improved glucose tolerance and random blood sugar levels more than diet alone. The improved glucose tolerance with both drugs was due to a combination of a reduced fasting plasma sugar level and an improved tolerance to the glucose load. Treatment with glibenclamide increased insulin secretion but phenformin had no significant effect. Mean body weight fell slightly during phenformin administration, whereas a small but insignificant rise in patients receiving glibenclamide.

Blood Glucose↗
PubMed1975

Influence of treatment with diet alone on oral glucose-tolerance test and plasma sugar and insulin levels in patients with maturity-onset diabetes mellitus.

Oral glucose-tolerance test (O.G.T.T.) plasma sugar and insulin levels were measured in 118 newly diagnosed maturity-onset diabetic patients before and after treatment with diet alone for periods of 2 and 6 months. The results of glucose-tolerance tests carried out during treatment could be predicted from the initial test and the weight reduction between the tests. This prediction was not improved by the addition of further variables, including age, obesity, and plasma-insulin levels during the first test. The change in O.C.T.T. plasma-insulin between the first and second tests was predicted by the result of the initial tests, the improvement of glucose tolerance between the two tests, and the degree of weight reduction. 95% of the group achieved some improvement of glucose tolerance after 2 months of dietary treatment, and 59% of the group achieved adequate diabetic control by this time. It is concluded that treatment with diet alone should be the first-line management for patients with newly diagnosed maturity-onset diabetes mellitus.

Adult↗
PubMed1975

Glucose metabolism and insulin response in the plasma and CSF in motor neurone disease.

Plasma glucose and insulin values and insulin values were measured during an OGTT test in patients with MND and matched controls. CSF insulin and glucose levels were measured in the fasting state in 11 patients and 1 hr after a glucose load in 3 patients. There was no significant difference between the values found in the plasma or CSF of the MND patientsand controls. The possible mechansim causing anterior horn cell death in MND is discussed.

Blood Glucose↗