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Biomedical subjects

C Eder

Publications and source records attributed to C Eder.

64 records · Page 4Linked to original sources
PubMed1989

Increased frequency of detection of human papillomavirus deoxyribonucleic acid in exfoliated cervical cells during pregnancy.

Exfoliated cells of the uterine cervix obtained from women during pregnancy and at the time of their first postpartum examination were used to monitor the prevalence of human papillomavirus infections in this population and to study the natural fluctuations in viral expression. When deoxyribonucleic acid hybridization analysis alone was used to monitor the presence of human papillomavirus infection, 20.9% of our study population had results that were positive for human papillomavirus deoxyribonucleic acid during their first-trimester examinations. A dramatic increase in the percentage of women with positive results for human papillomavirus deoxyribonucleic acid was observed at the time of the patients' third-trimester examinations (46%). The overall increase in human papillomavirus-positive patients was a combination of a small number of patients who had positive results on their first examination and negative results on their second examination, and a larger number of patients who had negative results on their first-trimester examination and positive results for human papillomavirus deoxyribonucleic acid in the exfoliated cervical cells at the time of their third-trimester examination. The total percentage of patients with positive results for human papillomavirus deoxyribonucleic acid in their cervical cells at one or both assay points during pregnancy was 52.5%. Samples obtained at the postpartum examination demonstrated a dramatic decrease in the number of samples positive for human papillomavirus deoxyribonucleic acid (17.5%). This result was a combination of a large decrease in human papillomavirus-positive patients coupled with a small increase in detectable levels of human papillomavirus deoxyribonucleic acid in cervical samples from patients who had negative results on their previous examination. This study demonstrates a very high level of detectable human papillomavirus deoxyribonucleic acid in exfoliated cervical cells obtained during pregnancy and shows that the detectable levels of human papillomavirus deoxyribonucleic acid fluctuate during pregnancy.

Cervix Uteri↗
PubMed1989

Mechanism for human papillomavirus transmission at birth.

We attempted to investigate mechanisms, in addition to sexual contact, by which human papillomaviruses associated with anogenital tract lesions could be transmitted. Samples of exfoliated cervical cells were obtained from 45 pregnant women and were assayed by Southern blot hybridization analysis for the presence of human papillomavirus nucleic acids. Twenty-five of the 45 women had cells positive for human papillomavirus deoxyribonucleic acid. A neonatal nasopharyngeal aspirate was obtained at term and analyzed for the presence of human papillomavirus deoxyribonucleic acid. We documented the presence of human papillomavirus deoxyribonucleic acid in the oral pharyngeal cavity of the neonates in 15 of 45 nasopharyngeal samples analyzed. Amniotic fluid was obtained from 13 patients when their membranes were artificially ruptured. These samples were assayed for the presence of human papillomavirus deoxyribonucleic acid; two of the 13 amniotic fluid samples contained human papillomavirus deoxyribonucleic acid. The detection of human papillomavirus deoxyribonucleic acid in the oral cavity of neonates is indicative of a perinatal mechanism of viral transmission. The detection of human papillomavirus deoxyribonucleic acid in the amniotic fluid may suggest an in utero mechanism of transmission. However, problems encountered in collecting the amniotic fluid samples preclude us from definitive interpretation of these data.

Adult↗
PubMed1984

[Severe phosphate depletion in uncontrolled diabetes mellitus (case report)].

The appearance of the syndrome of phosphate depletion (plasma phosphorus level: 0,12 mmol/l) in a patient with uncontrolled diabetes mellitus is reported. The inorganic phosphorus is essential for the resynthesis of 2,3-DPG and ATP, therefore phosphate depletion results in tissue hypoxia and decrease of energy rich phosphate with disturbances of various organ systems. The causes, pathogenetic mechanisms and the clinical signs and symptoms are discussed. Regular controlls of plasma phosphorus levels and a prophylactic substitution of phosphate are recommended.

Adult↗
PubMed1984

[Reflux and stool frequency in the artificial feeding of traumatized intensive care patients].

The effect of intragastric nutrion and its complications, especially the reflux pattern, were studied in 104 patients, who had to be intensively treated for more than 10 days. Patients suffering from polytrauma without abdominal injury (group I) or with abdominal injury (group III) and patients with isolated head/brain injury (group II) were included in this study. Parenteral nutrition was changed to intragastric administration as soon as possible and enteral nutrition was softly increased from 327 calories per day during the first week to 1800 calories or more in the next weeks. The general duration of feeding by nasogastric tube was between 16,1 and 19,8 days. The occurrence of reflux varied from 23,2% in patients of group II to 29,4% in patients of group I. Significant changes of reflux pattern were seen during the different weeks of treatment. The amount of reflux fluid was between 184,8 ml in group II and 355,4 ml in group III. The percentage of reflux fluid, if compared with the total enteral fluid administration revealed the maximum (16,6%) in patients of group III. Patients in group I had the highest frequency of defecation, the highest incidence of diarrhea was in all the groups really the same. Diarrhoea was seen in 6,7% of all patients. The underlying mechanism for these complications were discussed.

Abdominal Injuries↗
PubMed1983

Experimental non-A, non-B hepatitis in chimpanzees: light, electron and immune microscopical observations.

Two chimpanzees (Pan troglodytes) were inoculated and cross-challenged with a fibrinogen and factor VIII preparation, respectively. Successful non-A, non-B (NANB) infection was documented by biphasic elevations of aminotransferases (ALT), concomitant hepatitic reactions and typical electron microscopic alterations, the most consistent being dilatation of the endoplasmic reticulum, as well as tubular and sponge-like cytoplasmic inclusions in the absence of nuclear virus-like particles. An anti-nuclear (anti-DNA) antibody of the IgM class in one of the chimpanzees simulating an antiviral antibody is described.

Animals↗
PubMedDate not supplied

[Ambulation training with bilateral limited weight bearing after foot injuries].

AIM: Bilateral foot injuries are not uncommon. Operative treatment usually requires partial weight bearing over several weeks. This is a challenge for physiotherapy especially for ambulation training. We describe a new rehabilitation aid allowing for bilateral limited weight bearing during ambulation. METHODS: Ambulation training was initiated in the early postoperative period using the Regensburg ambulation aid. A lift system allows for exact limitation of weight bearing on both sides controlled by balances integrated into the floor. Using a rail system a 15 m free walking distance is available. This study included 10 patients with bilateral foot fractures. The functional outcome was assessed using the AOFAS score and quality of life with the SF-36 score. Plain X-rays were used to evaluate loss of correction. The control group consisted of 20 patients where ambulation training was discontinued for at least 6 weeks postoperatively. RESULTS: The system is very safe, during the entire period no complication was recorded. Acceptance among the patients was extremely high. Worries about unwanted weight bearing could be reduced. Patients mobilized with early ambulation training showed a better compensation of gait if compared to the control group. Secondary problems in the knee, hip or spine, as well as muscular deficiencies had a significantly lower incidence. No loss of correction could be seen in either of the two groups. CONCLUSION: The presented ambulation aid is a significant contribution to the physiotherapy of bilateral foot injuries.

Equipment Design↗
PubMedDate not supplied

Independent prognostication and therapy monitoring of breast cancer patients by DNA/RNA typing of minimal residual cancer cells.

Clinical relevance, purification techniques and molecular characterization of minimal residual cancer cells (MRCC) is a controversial topic in the literature. An analytical concept including a novel isolation procedure and a panel of tests for DNA and RNA typing of MRCCs is described and clinically evaluated in this paper. The purification procedure exploiting the physical characteristics of MRCCs shows superior performance leading to > 50% pure and viable tumor cells. Proof of the presence and purity of MRCCs in an isolated sample is given by multiparametric DNA typing (amplifications, mutations, losses of heterozygosity). On the basis of the proven presence of MRCCs tumor-relevant mRNAs can be adequately analyzed by normalized quantitative real-time RT-PCR. The molecular characterization of MRCCs isolated from blood of breast cancer patients could have a strong clinical impact on prognostication, drug targeting and therapy monitoring.

Biomarkers, Tumor↗
PubMedDate not supplied

Intracytoplasmic crystalline inclusions in the hepatocytes of humans and chimpanzees.

Intracytoplasmic crystalline inclusions (CCIs) were observed in liver biopsy specimens taken from three patients and three chimpanzees. CCIs are rare in human hepatocytes and have never been described in chimpanzees. All three human cases were connected with alcoholic liver disease; no viral infection was noted. The histologies of the two liver biopsy specimens obtained from healthy chimpanzees were normal, but hepatitis B virus (HBV) infection was demonstrated in the third case by immunohistochemistry and radioimmunoassay. In the last biopsy specimen a large accumulation of HBV core particles was present in the nuclei and among the filaments of CCIs of the hepatocytes. This finding suggests that crystallizations of viral proteins may form intracytoplasmic inclusions in hepatocytes, whereas in other cases toxic effects (alcohol) or normal conditions (storage) may lead to the formation of similar structures.

Animals↗