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C Ezpeleta

Publications and source records attributed to C Ezpeleta.

17 recordsLinked to original sources
PubMed1999

Detection of HGV in serum and peripheral blood mononuclear cells of maintenance haemodialysis patients.

The aim of the present study was to investigate the prevalence of hepatitis G virus (HGV) and also hepatitis C virus (HCV) infections in maintenance haemodialysis patients, and to identify extrahepatic sites as HGV reservoirs. HGV RNA was detected in the serum of 6/61 (10%) patients and in the peripheral blood mononuclear cells of 2/61 (3%) patients (one of whom was serum negative). These findings suggest that lymphoid cells constitute an extrahepatic HGV reservoir. HCV RNA was detected in 7/61 (11%) patients. Five of these patients (71%) were identified as carrying HCV genotype 1b. Co-infection with HCV and HGV was detected only in one patient. Haemodialysis patients are at risk for HGV infection, by nosocomial routes or via transfusions. HGV itself does not seem to be an important cause of hepatitis since all six HGV RNA positive patients not co-infected by HCV or HBV showed normal ALT values.

Adult↗
PubMed1998

[Genetic typing by PCR of isolates of C. albicans obtained in a resuscitation unit].

BACKGROUND: Typing by PCR (random polymorphic amplification or arbitrarily primed PCR) consists in a random amplification with the use of initiators of unknown homology with respect to the mold sequence. This study is of interest given the development of the technology of the amplification of nucleic acids and its application in the epidemiologic characterization of isolates of C. albicans. METHODS: Fourteen strains isolates in blood cultures of 8 patients were studied. All were identified as C. albicans. For amplification the sequence of AP3 and ERIC2 were selected. RESULTS: With one strain a band pattern very different from that obtained with the remaining isolates identified as C. albicans was achieved leading to reidentification and proving that it was C. parapsilopsis. On combining the results obtained with the use of both initiators 7 different genotypes were obtained with the remaining strains: A1, 2B, 3C, 4C, 5D, 6B and 7E. CONCLUSIONS: The discriminative power of the two initiators was similar although the AP3 was greater obtaining one more genotype than ERIC2. The patients with repeated yeast isolates over time which may be considered as the same episode of bacteremia, each presented the same band pattern and each was infected by one single clone. We herewith confirm the usefulness of typing by PCR with one initiator by reaction. The results may be improved with the combination of the profiles obtained with the use of several sequences if greater discrimination is required. Likewise, its use has shown to be satisfactory in both the identification of clones within one species and the identification of species within the genus.

Candida albicans↗
PubMed1996

Serratia rubidaea as an invasive pathogen.

Serratia rubidaea biotype 1 was isolated from the bile and blood of a patient with a bile tract carcinoma obstructing the common bile duct and who underwent invasive procedures. The infection was cleared after adequate treatment with antibiotics.

Anti-Bacterial Agents↗
PubMed1991

A "Pap" test for men? Male urethral smears as screening tool for detecting subclinical human papillomavirus infection.

We have explored the potential of using immunoperoxidase staining and in situ DNA hybridization techniques to detect the HPV common antigen and HPV DNA (subtypes 6/11 and 16/18) in urethral smears obtained from men with no grossly visible urethral lesions, with or without HPV-related clinical history. A total of 91 such smears were studied of which 25 (27.5%) were positive for HPV antigen. Of the latter, 20 (80%) were positive for HPV 6/11 DNA, 3 (12%) were positive for HPV 16/18 DNA, one smear (4%) was positive for both, and one was negative for both. The technique of obtaining male urethral smears and immunostaining them for HPV antigen is simple, rapid, specific, and far more sensitive than conventional morphologic observations. Since antigen-positive cells are the source for spreading HPV infection, identifying individuals with such cells can help limit the spread of infection by alerting sexual partners, and also can be used to monitor the infectious status of the patient after therapy. DNA subtyping of HPV-positive patients may be useful in predicting the potential for malignant transformation which varies among the different HPV subtypes.

Antigens, Viral↗
PubMed1991

[Splenic abscess: clinico-microbiologic study of 15 cases].

We have reviewed 15 cases of splenic abscesses diagnosed using a histopathological and microbiological study. Mean age was 48 years (range 18-78). Eight of them were male and 7 female. Eighty per cent of them had predisposing diseases, amongst which the most frequent were endocarditis and bacteriemia of other origin (26.6% in both cases). All patients presented fever and in 7 of them (47%) splenomegaly was appreciated. The most frequently isolated germs were gramnegative bacteria (33%), anaerobics (20%), and with a similar incidence grampositive bacteria, tuberculosis and fungii; in only one case cultures were sterile. Seven patients had multiple splenic abscesses and 8 patients single abscesses. Thorax x-ray showed alterations in more than half of the patients (53%). Abdominal echography was the diagnostic method in 67% of the patients and TAC in all the cases in which it was performed. Eight patients underwent splenectomy and one was surgically drained being the mortality rate of this group of 22%. Overall mortality was 33% and was related to the presence of multiple splenic abscesses (5/7, 71.4%), positive hemocultures (6/6, 100%), extrasplenic abscesses (7/8, 87.5%), and endocarditis (4/4, 100%), fungii infections (2/2, 100%), and late diagnosis (6/6, 100%).

Abscess↗
PubMed1988

Blistering distal dactylitis in an adult.

Streptococci are responsible for a diverse spectrum of cutaneous infections like erysipelas, impetigo, and cellulitis. Blistering distal dactylitis is an infrequent, superficial infection of the anterior fat pad of the distal portion of the finger described classically in children. We report a case of blistering distal dactylitis in an adult caused by group A beta-hemolytic streptococci that was cured with oral phenoxymethyl penicillin. Familiarity with blistering distal dactylitis should permit the dermatologist and internist to diagnose this new entity in adults.

Adult↗