The distribution of aspartic acid residues in bovine dentine phosphoprotein.
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Amino Acid Sequence↗
Biomedical subjects
C F Nawrot
Publications and source records attributed to C F Nawrot.
A chromatographic study of the relative affinities of rat bone and skin collagen alpha1 chains for hydroxyapatite.
The affinity of a bone collagen alpha1 chain for hydroxyapatite has been compared to a similarly isolated skin component from the same animals. The chain from bone exhibits a higher affinity for the mineral. This enhanced affinity appears to be related to its primary structure, specifically its hydroxylysine moieties.
Age Factors↗
Dental phosphoprotein-induced formation of hydroxylapatite during in vitro synthesis of amorphous calcium phosphate.
(Ethylenedinitrilo)tetraacetic acid soluble phosphoproteins were isolated from rat incisor and bovine unerupted teeth. This material was examined for its effect on the stability of amorphous calcium phosphate in vitro. When the precipitation of amorphous calcium phosphate was attempted in the presence of small amounts of these phospho-proteins, an apatite-like mineral was observed to form, which was approximately 60% crystalline, as determined by infrared measurements. This apatite phase could not be induced by addition of phosphoprotein after the precipitation reaction. The organic phosphate bound to these phosphoproteins was shown to be directly responsible for the formation of the apatite phase, since removal of 60% of the covalently bound phosphate with alkaline phosphatase destroyed the protein's ability to induce hydroxylapatite formation. The properties of the dental phosphoproteins appear to be consistent with their possible involvement in the development of the mineral phase of dentine.
Amino Acids↗
Studies on matrix proteins of normal and lathyritic rat bone and dentine.
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Amino Acids↗
Basicity differences among peptide bonds.
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Electric Conductivity↗
The mutarotation of poly-L-hydroxyproline.
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Chemical Phenomena↗
Chromatographic properties of denatured skin and bone collagens on hydroxyapatite.
Denatured collagen samples extracted from the skin and bone of lathyritic rats with guanidine hydrochloride were chromatographed on hydroxyapatite columns. A major fraction of the bone extract that was rich in alpha chain showed a stronger binding to hydroxyapatite when compared to the corresponding fraction from skin. Thus, bone collagen shows a special affinity for hydroxyapatite after isolation and denaturation.
Amino Acids↗